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B3A0S5

- XYNA_FUSO4

UniProt

B3A0S5 - XYNA_FUSO4

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Protein

Endo-1,4-beta-xylanase A

Gene

FOXG_17421

Organism
Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936) (Fusarium vascular wilt of tomato)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of the internal glycosidic bonds in heteroxylans, releasing mainly xylobiose and xylotriose. Most active on oat-spelt xylan.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.1 Publication

Kineticsi

    Vmax=1.22 mmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 6-8.1 Publication

    Temperature dependencei

    Optimum temperature is 40-50 degrees Celsius.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei131 – 1311Proton donor1 Publication
    Active sitei245 – 2451Nucleophile1 PublicationPROSITE-ProRule annotation

    GO - Molecular functioni

    1. endo-1,4-beta-xylanase activity Source: UniProtKB-EC

    GO - Biological processi

    1. xylan catabolic process Source: UniProtKB-UniPathway
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Enzyme and pathway databases

    UniPathwayiUPA00114.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,4-beta-xylanase A (EC:3.2.1.8)
    Short name:
    Xylanase A
    Alternative name(s):
    1,4-beta-D-xylan xylanohydrolase A
    Endo-1,4-beta-xylanase GH10 A
    Short name:
    FoXyn10a
    Xylanase III
    Gene namesi
    ORF Names:FOXG_17421
    OrganismiFusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936) (Fusarium vascular wilt of tomato)
    Taxonomic identifieri426428 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium oxysporum species complex
    ProteomesiUP000009097: Unplaced

    Subcellular locationi

    Secretedextracellular space 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327Endo-1,4-beta-xylanase APRO_0000415231Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi81 ↔ 1231 Publication
    Glycosylationi101 – 1011N-linked (GlcNAc...)1 Publication
    Disulfide bondi273 ↔ 2791 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    Secondary structure

    1
    327
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 117Combined sources
    Beta strandi16 – 216Combined sources
    Helixi28 – 336Combined sources
    Turni36 – 383Combined sources
    Beta strandi41 – 466Combined sources
    Helixi50 – 534Combined sources
    Helixi63 – 7311Combined sources
    Turni74 – 763Combined sources
    Beta strandi78 – 8710Combined sources
    Helixi92 – 954Combined sources
    Helixi101 – 11818Combined sources
    Turni119 – 1224Combined sources
    Beta strandi124 – 1307Combined sources
    Beta strandi137 – 1393Combined sources
    Helixi143 – 1486Combined sources
    Helixi152 – 16312Combined sources
    Beta strandi167 – 1759Combined sources
    Helixi181 – 19515Combined sources
    Beta strandi202 – 2054Combined sources
    Beta strandi208 – 2125Combined sources
    Helixi224 – 23512Combined sources
    Turni236 – 2383Combined sources
    Beta strandi240 – 25314Combined sources
    Helixi256 – 27520Combined sources
    Beta strandi279 – 2857Combined sources
    Helixi289 – 2913Combined sources
    Helixi294 – 2974Combined sources
    Beta strandi305 – 3073Combined sources
    Helixi315 – 32511Combined sources

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3U7BX-ray1.94A/B/C/D/E1-327[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 10 (cellulase F) family.Sequence Analysis

    Phylogenomic databases

    OrthoDBiEOG7GJ6PM.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001000. Glyco_hydro_10.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00331. Glyco_hydro_10. 1 hit.
    [Graphical view]
    PRINTSiPR00134. GLHYDRLASE10.
    SMARTiSM00633. Glyco_10. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B3A0S5-1 [UniParc]FASTAAdd to Basket

    « Hide

            10         20         30         40         50
    AASGLEAAMK AAGKQYFGTA LTVRNDQGEI DIINNKNEIG SITPENAMKW
    60 70 80 90 100
    EAIQPNRGQF NWGPADQHAA AATSRGYELR CHTLVWHSQL PSWVANGNWN
    110 120 130 140 150
    NQTLQAVMRD HINAVMGRYR GKCTHWDVVN EALNEDGTYR DSVFLRVIGE
    160 170 180 190 200
    AYIPIAFRMA LAADPTTKLY YNDYNLEYGN AKTEGAKRIA RLVKSYGLRI
    210 220 230 240 250
    DGIGLQAHMT SESTPTQNTP TPSRAKLASV LQGLADLGVD VAYTELDIRM
    260 270 280 290 300
    NTPATQQKLQ TNADAYARIV GSCMDVKRCV GITVWGISDK YSWVPGTFPG
    310 320
    EGSALLWNDN FQKKPSYTST LNTINRR
    Length:327
    Mass (Da):36,198
    Last modified:January 25, 2012 - v1
    Checksum:iDC4D2D291A37E7A0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAXH01001232 Genomic DNA. No translation available.

    Genome annotation databases

    EnsemblFungiiFOXG_17421T0; FOXG_17421P0; FOXG_17421.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAXH01001232 Genomic DNA. No translation available.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3U7B X-ray 1.94 A/B/C/D/E 1-327 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii FOXG_17421T0 ; FOXG_17421P0 ; FOXG_17421 .

    Phylogenomic databases

    OrthoDBi EOG7GJ6PM.

    Enzyme and pathway databases

    UniPathwayi UPA00114 .

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001000. Glyco_hydro_10.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00331. Glyco_hydro_10. 1 hit.
    [Graphical view ]
    PRINTSi PR00134. GLHYDRLASE10.
    SMARTi SM00633. Glyco_10. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium."
      Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J., Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B., Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R., Antoniw J., Baker S.E.
      , Bluhm B.H., Breakspear A., Brown D.W., Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J., Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E., Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M., Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D., Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A., Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C., Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S., Galagan J., Cuomo C.A., Kistler H.C., Rep M.
      Nature 464:367-373(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 25-327.
      Strain: 4287 / CBS 123668 / FGSC 9935 / NRRL 34936.
    2. "The alkaline xylanase III from Fusarium oxysporum F3 belongs to family F/10."
      Christakopoulos P., Nerinckx W., Kekos D., Macris B., Claeyssens M.
      Carbohydr. Res. 302:191-195(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 168-182 AND 290-313, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION.
      Strain: F3.
    3. "The structure of a GH10 xylanase from Fusarium oxysporum reveals the presence of an extended loop on top of the catalytic cleft."
      Dimarogona M., Topakas E., Christakopoulos P., Chrysina E.D.
      Acta Crystallogr. 68:735-742(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS), PROTEIN SEQUENCE OF 1-24, GLYCOSYLATION AT ASN-101, ACTIVE SITES, DISULFIDE BONDS.
      Strain: F3.

    Entry informationi

    Entry nameiXYNA_FUSO4
    AccessioniPrimary (citable) accession number: B3A0S5
    Secondary accession number(s): J9NQE2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: January 25, 2012
    Last modified: October 29, 2014
    This is version 20 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3