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Protein

Endo-1,4-beta-xylanase A

Gene

FOXG_17421

Organism
Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936) (Fusarium vascular wilt of tomato)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of the internal glycosidic bonds in heteroxylans, releasing mainly xylobiose and xylotriose. Most active on oat-spelt xylan.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.1 Publication

Kineticsi

    1. Vmax=1.22 mmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 6-8.1 Publication

    Temperature dependencei

    Optimum temperature is 40-50 degrees Celsius.1 Publication

    Pathwayi: xylan degradation

    This protein is involved in the pathway xylan degradation, which is part of Glycan degradation.
    View all proteins of this organism that are known to be involved in the pathway xylan degradation and in Glycan degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei131Proton donor1 Publication1
    Active sitei245NucleophilePROSITE-ProRule annotation1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    Biological processCarbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Enzyme and pathway databases

    UniPathwayiUPA00114

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,4-beta-xylanase A (EC:3.2.1.8)
    Short name:
    Xylanase A
    Alternative name(s):
    1,4-beta-D-xylan xylanohydrolase A
    Endo-1,4-beta-xylanase GH10 A
    Short name:
    FoXyn10a
    Xylanase III
    Gene namesi
    ORF Names:FOXG_17421
    OrganismiFusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936) (Fusarium vascular wilt of tomato)
    Taxonomic identifieri426428 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium oxysporum species complex
    Proteomesi
    • UP000009097 Componenti: Unplaced

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004152311 – 327Endo-1,4-beta-xylanase AAdd BLAST327

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi81 ↔ 1231 Publication
    Glycosylationi101N-linked (GlcNAc...) asparagine1 Publication1
    Disulfide bondi273 ↔ 2791 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiB3A0S5

    PTM databases

    iPTMnetiB3A0S5

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi5507.FOXG_17421P0

    Structurei

    Secondary structure

    1327
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi5 – 11Combined sources7
    Beta strandi16 – 21Combined sources6
    Helixi28 – 33Combined sources6
    Turni36 – 38Combined sources3
    Beta strandi41 – 46Combined sources6
    Helixi50 – 53Combined sources4
    Helixi63 – 73Combined sources11
    Turni74 – 76Combined sources3
    Beta strandi78 – 87Combined sources10
    Helixi92 – 95Combined sources4
    Helixi101 – 118Combined sources18
    Turni119 – 122Combined sources4
    Beta strandi124 – 130Combined sources7
    Beta strandi137 – 139Combined sources3
    Helixi143 – 148Combined sources6
    Helixi152 – 163Combined sources12
    Beta strandi167 – 175Combined sources9
    Helixi181 – 195Combined sources15
    Beta strandi202 – 205Combined sources4
    Beta strandi208 – 212Combined sources5
    Helixi224 – 235Combined sources12
    Turni236 – 238Combined sources3
    Beta strandi240 – 253Combined sources14
    Helixi256 – 275Combined sources20
    Beta strandi279 – 285Combined sources7
    Helixi289 – 291Combined sources3
    Helixi294 – 297Combined sources4
    Beta strandi305 – 307Combined sources3
    Helixi315 – 325Combined sources11

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3U7BX-ray1.94A/B/C/D/E1-327[»]
    SMRiB3A0S5
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini1 – 323GH10PROSITE-ProRule annotationAdd BLAST323

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 10 (cellulase F) family.Sequence analysis

    Phylogenomic databases

    OrthoDBiEOG092C45ID

    Family and domain databases

    InterProiView protein in InterPro
    IPR001000 GH10
    IPR017853 Glycoside_hydrolase_SF
    PfamiView protein in Pfam
    PF00331 Glyco_hydro_10, 1 hit
    PRINTSiPR00134 GLHYDRLASE10
    SMARTiView protein in SMART
    SM00633 Glyco_10, 1 hit
    SUPFAMiSSF51445 SSF51445, 1 hit
    PROSITEiView protein in PROSITE
    PS51760 GH10_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    B3A0S5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    AASGLEAAMK AAGKQYFGTA LTVRNDQGEI DIINNKNEIG SITPENAMKW
    60 70 80 90 100
    EAIQPNRGQF NWGPADQHAA AATSRGYELR CHTLVWHSQL PSWVANGNWN
    110 120 130 140 150
    NQTLQAVMRD HINAVMGRYR GKCTHWDVVN EALNEDGTYR DSVFLRVIGE
    160 170 180 190 200
    AYIPIAFRMA LAADPTTKLY YNDYNLEYGN AKTEGAKRIA RLVKSYGLRI
    210 220 230 240 250
    DGIGLQAHMT SESTPTQNTP TPSRAKLASV LQGLADLGVD VAYTELDIRM
    260 270 280 290 300
    NTPATQQKLQ TNADAYARIV GSCMDVKRCV GITVWGISDK YSWVPGTFPG
    310 320
    EGSALLWNDN FQKKPSYTST LNTINRR
    Length:327
    Mass (Da):36,198
    Last modified:January 25, 2012 - v1
    Checksum:iDC4D2D291A37E7A0
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AAXH01001232 Genomic DNA No translation available.

    Similar proteinsi

    Entry informationi

    Entry nameiXYNA_FUSO4
    AccessioniPrimary (citable) accession number: B3A0S5
    Secondary accession number(s): J9NQE2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: January 25, 2012
    Last modified: May 23, 2018
    This is version 36 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health