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B3A0S5

- XYNA_FUSO4

UniProt

B3A0S5 - XYNA_FUSO4

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Protein
Endo-1,4-beta-xylanase A
Gene
FOXG_17421
Organism
Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936) (Fusarium vascular wilt of tomato)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of the internal glycosidic bonds in heteroxylans, releasing mainly xylobiose and xylotriose. Most active on oat-spelt xylan.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.2 Publications

Kineticsi

    Vmax=1.22 mmol/min/mg enzyme2 Publications

    pH dependencei

    Optimum pH is 6-8.1 Publication

    Temperature dependencei

    Optimum temperature is 40-50 degrees Celsius.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei131 – 1311Proton donor1 Publication
    Active sitei245 – 2451Nucleophile1 Publication

    GO - Molecular functioni

    1. endo-1,4-beta-xylanase activity Source: UniProtKB-EC

    GO - Biological processi

    1. xylan catabolic process Source: UniProtKB-UniPathway
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Enzyme and pathway databases

    UniPathwayiUPA00114.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,4-beta-xylanase A (EC:3.2.1.8)
    Short name:
    Xylanase A
    Alternative name(s):
    1,4-beta-D-xylan xylanohydrolase A
    Endo-1,4-beta-xylanase GH10 A
    Short name:
    FoXyn10a
    Xylanase III
    Gene namesi
    ORF Names:FOXG_17421
    OrganismiFusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936) (Fusarium vascular wilt of tomato)
    Taxonomic identifieri426428 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium oxysporum species complex
    ProteomesiUP000009097: Unplaced

    Subcellular locationi

    Secretedextracellular space 2 Publications

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327Endo-1,4-beta-xylanase A
    PRO_0000415231Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi81 ↔ 1231 Publication
    Glycosylationi101 – 1011N-linked (GlcNAc...)1 Publication
    Disulfide bondi273 ↔ 2791 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Monomer.2 Publications

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 117
    Beta strandi16 – 216
    Helixi28 – 336
    Turni36 – 383
    Beta strandi41 – 466
    Helixi50 – 534
    Helixi63 – 7311
    Turni74 – 763
    Beta strandi78 – 8710
    Helixi92 – 954
    Helixi101 – 11818
    Turni119 – 1224
    Beta strandi124 – 1307
    Beta strandi137 – 1393
    Helixi143 – 1486
    Helixi152 – 16312
    Beta strandi167 – 1759
    Helixi181 – 19515
    Beta strandi202 – 2054
    Beta strandi208 – 2125
    Helixi224 – 23512
    Turni236 – 2383
    Beta strandi240 – 25314
    Helixi256 – 27520
    Beta strandi279 – 2857
    Helixi289 – 2913
    Helixi294 – 2974
    Beta strandi305 – 3073
    Helixi315 – 32511

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3U7BX-ray1.94A/B/C/D/E1-327[»]

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    OrthoDBiEOG7GJ6PM.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001000. Glyco_hydro_10.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00331. Glyco_hydro_10. 1 hit.
    [Graphical view]
    PRINTSiPR00134. GLHYDRLASE10.
    SMARTiSM00633. Glyco_10. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B3A0S5-1 [UniParc]FASTAAdd to Basket

    « Hide

    AASGLEAAMK AAGKQYFGTA LTVRNDQGEI DIINNKNEIG SITPENAMKW    50
    EAIQPNRGQF NWGPADQHAA AATSRGYELR CHTLVWHSQL PSWVANGNWN 100
    NQTLQAVMRD HINAVMGRYR GKCTHWDVVN EALNEDGTYR DSVFLRVIGE 150
    AYIPIAFRMA LAADPTTKLY YNDYNLEYGN AKTEGAKRIA RLVKSYGLRI 200
    DGIGLQAHMT SESTPTQNTP TPSRAKLASV LQGLADLGVD VAYTELDIRM 250
    NTPATQQKLQ TNADAYARIV GSCMDVKRCV GITVWGISDK YSWVPGTFPG 300
    EGSALLWNDN FQKKPSYTST LNTINRR 327
    Length:327
    Mass (Da):36,198
    Last modified:January 25, 2012 - v1
    Checksum:iDC4D2D291A37E7A0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAXH01001232 Genomic DNA. No translation available.

    Genome annotation databases

    EnsemblFungiiFOXG_17421T0; FOXG_17421P0; FOXG_17421.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAXH01001232 Genomic DNA. No translation available.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3U7B X-ray 1.94 A/B/C/D/E 1-327 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii FOXG_17421T0 ; FOXG_17421P0 ; FOXG_17421 .

    Phylogenomic databases

    OrthoDBi EOG7GJ6PM.

    Enzyme and pathway databases

    UniPathwayi UPA00114 .

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001000. Glyco_hydro_10.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00331. Glyco_hydro_10. 1 hit.
    [Graphical view ]
    PRINTSi PR00134. GLHYDRLASE10.
    SMARTi SM00633. Glyco_10. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium."
      Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J., Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B., Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R., Antoniw J., Baker S.E.
      , Bluhm B.H., Breakspear A., Brown D.W., Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J., Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E., Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M., Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D., Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A., Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C., Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S., Galagan J., Cuomo C.A., Kistler H.C., Rep M.
      Nature 464:367-373(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 25-327.
      Strain: 4287 / CBS 123668 / FGSC 9935 / NRRL 34936.
    2. "The alkaline xylanase III from Fusarium oxysporum F3 belongs to family F/10."
      Christakopoulos P., Nerinckx W., Kekos D., Macris B., Claeyssens M.
      Carbohydr. Res. 302:191-195(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 168-182 AND 290-313, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION.
      Strain: F3.
    3. "The structure of a GH10 xylanase from Fusarium oxysporum reveals the presence of an extended loop on top of the catalytic cleft."
      Dimarogona M., Topakas E., Christakopoulos P., Chrysina E.D.
      Acta Crystallogr. 68:735-742(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS), PROTEIN SEQUENCE OF 1-24, GLYCOSYLATION AT ASN-101, ACTIVE SITES, DISULFIDE BONDS.
      Strain: F3.

    Entry informationi

    Entry nameiXYNA_FUSO4
    AccessioniPrimary (citable) accession number: B3A0S5
    Secondary accession number(s): J9NQE2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: January 25, 2012
    Last modified: May 14, 2014
    This is version 18 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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