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B3A0N5

- APY_TABYA

UniProt

B3A0N5 - APY_TABYA

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Protein

Apyrase

Gene
N/A
Organism
Tabanus yao (Horsefly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Facilitates hematophagy by inhibiting ADP-dependent platelet aggregation in the host. Shows potential for antithrombotic activity. May reduce probing time by facilitating the speed of locating blood.1 Publication

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cations.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431Divalent metal cation 1By similarity
Metal bindingi45 – 451Divalent metal cation 1By similarity
Metal bindingi92 – 921Divalent metal cation 1By similarity
Metal bindingi92 – 921Divalent metal cation 2By similarity
Metal bindingi124 – 1241Divalent metal cation 2By similarity
Sitei125 – 1251Transition state stabilizerBy similarity
Sitei128 – 1281Transition state stabilizerBy similarity
Metal bindingi224 – 2241Divalent metal cation 2By similarity
Metal bindingi248 – 2481Divalent metal cation 2By similarity
Binding sitei418 – 4181SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity, acting on ester bonds Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. nucleotide catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apyrase1 Publication (EC:3.6.1.5By similarity)
Alternative name(s):
ATP-diphosphataseBy similarity
Short name:
ADPaseBy similarity
ATP-diphosphohydrolaseBy similarity
Adenosine diphosphataseBy similarity
Allergen: Tab y 11 Publication
OrganismiTabanus yao (Horsefly)
Taxonomic identifieri485572 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraTabanomorphaTabanoideaTabanidaeTabanus

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei9494. Tab y 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 554533ApyraseSequence AnalysisPRO_0000414929Add
BLAST

Expressioni

Tissue specificityi

Salivary gland specific.1 Publication

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni504 – 5107Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Sequence Analysis

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B3A0N5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFKITVFIYV LQLILPSKVH SSPVPDSDNG LREFPLSIVH INDFHARFEQ
60 70 80 90 100
TDELGGQCKP TAKCVGGYAR LVTTVKKLKE EGQNTIFLNA ADNYQGTLWY
110 120 130 140 150
NLGKWNVTAY FMNLLPADAM TLGNHEFDDK IEGIVPFLEV IKTPIVVANI
160 170 180 190 200
DDSLEPTFKG KYTKSVVLER GGRKIGIVGV IAQNTDNISS PGKLRFLDEI
210 220 230 240 250
QSVKNESKRL REEEKVDIVI VLSHIGLDHD YDLAEQAGDY IDAIIGGHSH
260 270 280 290 300
SFLWTGDNPP GKEKVVDAYP VEIVQTSGKK VLIVQASAFA RYVGNITLYF
310 320 330 340 350
GENNNLIRYA GAPVYLDSDV PEVPQIVEEM KAWEEFVHEK GNEIIAESRV
360 370 380 390 400
VLSRENCRVS DCNIGNFFTD AYVHEYVTSH TGPYWTPVSV GLMNVGGIRA
410 420 430 440 450
SVDRGNITFS QLITMAPFEN TVDTFDLSGK HLLEAFEHAV TVPNRLGFNG
460 470 480 490 500
QNMLQVSGVK LVYDVTKCEG QRVVSAKIRC QKCDIPKYEP LDPEETYRIV
510 520 530 540 550
TASFLANGGD GFTMIRDNKK NYKVGRKDYD VLINYAKYSS PITIGEEGRI

RIIQ
Length:554
Mass (Da):61,880
Last modified:January 25, 2012 - v1
Checksum:i99E4B3B04F38EF59
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti444 – 4441N → K AA sequence (PubMed:21848516)1 Publication

Cross-referencesi

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 9494. Tab y 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProi IPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
PANTHERi PTHR11575. PTHR11575. 1 hit.
Pfami PF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR01607. APYRASEFAMLY.
SUPFAMi SSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEi PS00785. 5_NUCLEOTIDASE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies."
    An S., Ma D., Wei J.F., Yang X., Yang H.W., Yang H., Xu X., He S., Lai R.
    Allergy 66:1420-1427(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 78-104; 143-159; 175-192; 292-308; 431-444 AND 538-549, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALLERGEN.
    Tissue: Salivary gland1 Publication.

Entry informationi

Entry nameiAPY_TABYA
AccessioniPrimary (citable) accession number: B3A0N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 25, 2012
Last modified: November 26, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3