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B3A0N5

- APY_TABYA

UniProt

B3A0N5 - APY_TABYA

Protein

Apyrase

Gene
N/A
Organism
Tabanus yao (Horsefly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 14 (01 Oct 2014)
      Sequence version 1 (25 Jan 2012)
      Previous versions | rss
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    Functioni

    Facilitates hematophagy by inhibiting ADP-dependent platelet aggregation in the host. Shows potential for antithrombotic activity. May reduce probing time by facilitating the speed of locating blood.1 Publication

    Catalytic activityi

    A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

    Cofactori

    Divalent metal cations.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi43 – 431Divalent metal cation 1By similarity
    Metal bindingi45 – 451Divalent metal cation 1By similarity
    Metal bindingi92 – 921Divalent metal cation 1By similarity
    Metal bindingi92 – 921Divalent metal cation 2By similarity
    Metal bindingi124 – 1241Divalent metal cation 2By similarity
    Sitei125 – 1251Transition state stabilizerBy similarity
    Sitei128 – 1281Transition state stabilizerBy similarity
    Metal bindingi224 – 2241Divalent metal cation 2By similarity
    Metal bindingi248 – 2481Divalent metal cation 2By similarity
    Binding sitei418 – 4181SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydrolase activity, acting on ester bonds Source: InterPro
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. nucleotide catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Apyrase1 Publication (EC:3.6.1.5By similarity)
    Alternative name(s):
    ATP-diphosphataseBy similarity
    Short name:
    ADPaseBy similarity
    ATP-diphosphohydrolaseBy similarity
    Adenosine diphosphataseBy similarity
    Allergen: Tab y 11 Publication
    OrganismiTabanus yao (Horsefly)
    Taxonomic identifieri485572 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraTabanomorphaTabanoideaTabanidaeTabanus

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human. Binds to IgE.1 Publication

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei9494. Tab y 1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 554533ApyraseSequence AnalysisPRO_0000414929Add
    BLAST

    Expressioni

    Tissue specificityi

    Salivary gland specific.1 Publication

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni504 – 5107Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the 5'-nucleotidase family.Sequence Analysis

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    3.90.780.10. 1 hit.
    InterProiIPR008334. 5'-Nucleotdase_C.
    IPR006146. 5'-Nucleotdase_CS.
    IPR006179. 5_nucleotidase/apyrase.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view]
    PANTHERiPTHR11575. PTHR11575. 1 hit.
    PfamiPF02872. 5_nucleotid_C. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR01607. APYRASEFAMLY.
    SUPFAMiSSF55816. SSF55816. 1 hit.
    SSF56300. SSF56300. 1 hit.
    PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B3A0N5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFKITVFIYV LQLILPSKVH SSPVPDSDNG LREFPLSIVH INDFHARFEQ    50
    TDELGGQCKP TAKCVGGYAR LVTTVKKLKE EGQNTIFLNA ADNYQGTLWY 100
    NLGKWNVTAY FMNLLPADAM TLGNHEFDDK IEGIVPFLEV IKTPIVVANI 150
    DDSLEPTFKG KYTKSVVLER GGRKIGIVGV IAQNTDNISS PGKLRFLDEI 200
    QSVKNESKRL REEEKVDIVI VLSHIGLDHD YDLAEQAGDY IDAIIGGHSH 250
    SFLWTGDNPP GKEKVVDAYP VEIVQTSGKK VLIVQASAFA RYVGNITLYF 300
    GENNNLIRYA GAPVYLDSDV PEVPQIVEEM KAWEEFVHEK GNEIIAESRV 350
    VLSRENCRVS DCNIGNFFTD AYVHEYVTSH TGPYWTPVSV GLMNVGGIRA 400
    SVDRGNITFS QLITMAPFEN TVDTFDLSGK HLLEAFEHAV TVPNRLGFNG 450
    QNMLQVSGVK LVYDVTKCEG QRVVSAKIRC QKCDIPKYEP LDPEETYRIV 500
    TASFLANGGD GFTMIRDNKK NYKVGRKDYD VLINYAKYSS PITIGEEGRI 550
    RIIQ 554
    Length:554
    Mass (Da):61,880
    Last modified:January 25, 2012 - v1
    Checksum:i99E4B3B04F38EF59
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti444 – 4441N → K AA sequence (PubMed:21848516)1 Publication

    Cross-referencesi

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 9494. Tab y 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    3.90.780.10. 1 hit.
    InterProi IPR008334. 5'-Nucleotdase_C.
    IPR006146. 5'-Nucleotdase_CS.
    IPR006179. 5_nucleotidase/apyrase.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view ]
    PANTHERi PTHR11575. PTHR11575. 1 hit.
    Pfami PF02872. 5_nucleotid_C. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR01607. APYRASEFAMLY.
    SUPFAMi SSF55816. SSF55816. 1 hit.
    SSF56300. SSF56300. 1 hit.
    PROSITEi PS00785. 5_NUCLEOTIDASE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies."
      An S., Ma D., Wei J.F., Yang X., Yang H.W., Yang H., Xu X., He S., Lai R.
      Allergy 66:1420-1427(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 78-104; 143-159; 175-192; 292-308; 431-444 AND 538-549, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALLERGEN.
      Tissue: Salivary gland1 Publication.

    Entry informationi

    Entry nameiAPY_TABYA
    AccessioniPrimary (citable) accession number: B3A0N5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: January 25, 2012
    Last modified: October 1, 2014
    This is version 14 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3