B3A003 (LYS3_CRAVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysozyme 3
EC=3.2.1.17

Alternative name(s):
1,4-beta-N-acetylmuramidase 3
cv-lysozyme 3

Gene names

Name:

lysoz3

Organism

Crassostrea virginica (Eastern oyster)

Taxonomic identifier

6565 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Bivalvia › Pteriomorphia › Ostreoida › Ostreoidea › Ostreidae › Crassostrea

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has antibacterial activity. Ref.2
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Ref.2
Subcellular location	Secreted By similarity UniProtKB P83673.
Sequence similarities	Belongs to the lysozyme type I family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.5-8.5. Ref.2
Mass spectrometry	Molecular mass is 17782.33 Da from positions 19 - 187. Determined by MALDI. Ref.2

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antibiotic Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.2
	Chain	19 – 187	169	Lysozyme 3 Ref.2		PRO_0000374069

Sequences

	Sequence		Length	Mass (Da)
	B3A003 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 688F67BD2DB2B2A5	FASTA	187	19,775
	10 20 30 40 50 60 MNGLFLFCVA TTAALAYGSD APCTNSGGVC QDDHLACHNG HYQSGLCTGG AHRRCCLTSA 70 80 90 100 110 120 SHTGSFSTGI VSQQCLQCIC NVESGCKAIG CHFDVNSDSC GYFQIKEGYW HDCGSPGSSW 130 140 150 160 170 180 RSCANDLACA SKCVQAYMSR YIGFSGCSHS CESYARIHNG GPAGCKHTNT LGYWSHVHAQ GCSHNSK

References

[1]	"Lysozyme 3 gene in the eastern oyster, Crassostrea virginica." Itoh N., Xue Q.-G., Schey K.L., Cooper R.K., La Peyre J.F. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cv-lysozyme 3, further evidence of adaptive evolution of i-type lysozymes for nutrition in the eastern oyster." Xue Q.-G., Itoh N., Schey K.L., Li Y., Cooper R.K., La Peyre J.F. Submitted (MAR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 19-53 AND 55-186, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MASS SPECTROMETRY. Tissue: Digestive gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB427186 mRNA. Translation: BAG41979.1.
3D structure databases
ProteinModelPortal	B3A003.
ModBase	Search...
Protein family/group databases
CAZy	GH22. Glycoside Hydrolase Family 22.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006080. Defensin_beta/neutrophil. IPR008597. Destabilase. IPR023346. Lysozyme-like_dom. [Graphical view]
PANTHER	PTHR11195. PTHR11195. 1 hit.
Pfam	PF05497. Destabilase. 1 hit. [Graphical view]
SMART	SM00048. DEFSN. 1 hit. [Graphical view]
SUPFAM	SSF53955. SSF53955. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LYS3_CRAVI

Accession

Primary (citable) accession number: B3A003
Secondary accession number(s): P85518

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	July 22, 2008
Last modified:	October 3, 2012
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)