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B2ZUN0

- B2ZUN0_9ENTO

UniProt

B2ZUN0 - B2ZUN0_9ENTO

Protein
Submitted name:

Polyprotein

Gene
N/A
Organism
Enterovirus A71
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    NTP + H2O = NDP + phosphate.SAAS annotation
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotation
    Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.SAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei593 – 5931AMP; via carbonyl oxygenImported

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. ion channel activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW
    5. RNA binding Source: UniProtKB-KW
    6. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    7. RNA helicase activity Source: InterPro
    8. structural molecule activity Source: InterPro

    GO - Biological processi

    1. pore formation by virus in membrane of host cell Source: UniProtKB-KW
    2. protein oligomerization Source: UniProtKB-KW
    3. RNA-protein covalent cross-linking Source: InterPro
    4. transcription, DNA-templated Source: InterPro
    5. viral entry into host cell Source: UniProtKB-KW
    6. viral RNA genome replication Source: InterPro
    7. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    HelicaseSAAS annotation, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-directed RNA polymeraseSAAS annotation, Thiol proteaseSAAS annotation, Transferase, Viral ion channelSAAS annotation

    Keywords - Biological processi

    Host-virus interaction, Ion transport, Transport, Viral attachment to host cellSAAS annotation, Viral RNA replicationSAAS annotation, Virus entry into host cell

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-bindingSAAS annotation

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    PolyproteinImported
    OrganismiEnterovirus A71Imported
    Taxonomic identifieri39054 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeEnterovirusEnterovirus A

    Subcellular locationi

    Host cytoplasm SAAS annotation
    Host cytoplasmic vesicle membrane SAAS annotation; Peripheral membrane protein SAAS annotation; Cytoplasmic side SAAS annotation

    GO - Cellular componenti

    1. host cell cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    2. integral to membrane of host cell Source: UniProtKB-KW
    3. membrane Source: UniProtKB-KW
    4. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid proteinSAAS annotation, Host cytoplasmSAAS annotation, Host cytoplasmic vesicleSAAS annotation, Host membraneSAAS annotation, Membrane, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6969VP4 (1A)ImportedPRO_5000373532Add
    BLAST
    Chaini70 – 323254VP2ImportedPRO_5000373533Add
    BLAST
    Chaini324 – 565242VP3ImportedPRO_5000373534Add
    BLAST
    Chaini566 – 862297VP1ImportedPRO_5000373535Add
    BLAST
    Chaini863 – 10121502AImportedPRO_5000373536Add
    BLAST
    Chaini1013 – 1111992BImportedPRO_5000373537Add
    BLAST
    Chaini1112 – 14403292CImportedPRO_5000373538Add
    BLAST
    Chaini1441 – 1526863AImportedPRO_5000373539Add
    BLAST
    Chaini1527 – 1548223BImportedPRO_5000373540Add
    BLAST
    Chaini1549 – 17311833CImportedPRO_5000373541Add
    BLAST
    Chaini1732 – 21934623DImportedPRO_5000373542Add
    BLAST

    Keywords - PTMi

    PhosphoproteinSAAS annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3J22electron microscopy6.30A638-862[»]
    B82-318[»]
    C324-562[»]
    3J23electron microscopy9.20A638-862[»]
    B82-318[»]
    C324-562[»]
    3VBFX-ray2.60A566-862[»]
    B79-323[»]
    C324-565[»]
    D12-69[»]
    3VBHX-ray2.30A566-862[»]
    B79-323[»]
    C324-565[»]
    D12-69[»]
    3VBOX-ray2.88A566-862[»]
    B82-318[»]
    C324-562[»]
    3VBRX-ray3.80A638-862[»]
    B82-318[»]
    C324-562[»]
    3VBSX-ray3.00A566-862[»]
    B79-323[»]
    C324-565[»]
    D12-69[»]
    3VBUX-ray4.00A638-862[»]
    B82-318[»]
    C324-562[»]
    4CDQX-ray2.65A566-862[»]
    B70-323[»]
    C324-565[»]
    D1-69[»]
    4CDUX-ray2.80A566-862[»]
    B70-323[»]
    C324-565[»]
    D1-69[»]
    4CDWX-ray2.80A566-862[»]
    B70-323[»]
    C324-565[»]
    D1-69[»]
    4CDXX-ray2.80A566-862[»]
    B70-323[»]
    C324-565[»]
    D1-69[»]
    4CEWX-ray2.75A566-862[»]
    B70-323[»]
    C324-565[»]
    D1-69[»]
    4CEYX-ray2.75A566-862[»]
    B70-323[»]
    C324-565[»]
    D1-69[»]
    4IKAX-ray2.70D1527-1548[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains RdRp catalytic domain.SAAS annotation
    Contains SF3 helicase domain.SAAS annotation
    Contains SFhelicase domain.SAAS annotation

    Family and domain databases

    Gene3Di2.60.120.20. 3 hits.
    3.40.50.300. 1 hit.
    4.10.80.10. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR014838. P3A.
    IPR000081. Peptidase_C3.
    IPR000199. Peptidase_C3A/C3B_picornavir.
    IPR003138. Pico_P1A.
    IPR002527. Pico_P2B.
    IPR001676. Picornavirus_capsid.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view]
    PfamiPF08727. P3A. 1 hit.
    PF00548. Peptidase_C3. 1 hit.
    PF02226. Pico_P1A. 1 hit.
    PF00947. Pico_P2A. 1 hit.
    PF01552. Pico_P2B. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00073. Rhv. 3 hits.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    ProDomiPD001306. Peptidase_C3. 1 hit.
    PD649346. Pico_P2B. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF89043. SSF89043. 1 hit.
    PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2ZUN0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSQVSTQRS GSHENSNSAT EGSTINYTTI NYYKDSYAAT AGKQSLKQDP     50
    DKFANPVKDI FTEMAAPLKS PSAEACGYSD RVAQLTIGNS TITTQEAANI 100
    IVGYGEWPSY CSDSDATAVD KPTRPDVSVN RFYTLDTKLW EKSSKGWYWK 150
    FPDVLTETGV FGQNAQFHYL YRSGFCIHVQ CNASKFHQGA LLVAVLPEYV 200
    IGTVAGGTGT EDTHPPYKQT QPGADGFELQ HPYVLDAGIP ISQLTVCPHQ 250
    WINLRTNNCA TIIVPYINAL PFDSALNHCN FGLLVVPISP LDYDQGATPV 300
    IPITITLAPM CSEFAGLRQA VTQGFPTELK PGTNQFLTTD DGVSAPILPN 350
    FHPTPCIHIP GEVRNLLELC QVETILEVNN VPTNATSLME RLRFPVSAQA 400
    GKGELCAVFR ADPGRNGPWQ STLLGQLCGY YTQWSGSLEV TFMFTGSFMA 450
    TGKMLIAYTP PGGPLPKDRA TAMLGTHVIW DFGLQSSVTL VIPWISNTHY 500
    RAHARDGVFD YYTTGLVSIW YQTNYVVPIG APNTAYIIAL AAAQKNFTMK 550
    LCKDASDILQ TGTIQGDRVA DVIESSIGDS VSRALTHALP APTGQNTQVS 600
    SHRLDTGKVP ALQAAEIGAS SNASDESMIE TRCVLNSHST AETTLDSFFS 650
    RAGLVGEIDL PLKGTTNPNG YANWDIDITG YAQMRRKVEL FTYMRFDAEF 700
    TFVACTPTGE VVPQLLQYMF VPPGAPKPDS RESLAWQTAT NPSVFVKLSD 750
    PPAQVSVPFM SPASAYQWFY DGYPTFGEHK QEKDLEYGAC PNNMMGTFSV 800
    RTVGTSKSKY PLVVRIYMRM KHVRAWIPRP MRNQNYLFKA NPNYAGNSIK 850
    PTGASRTAIT TLGKFGQQSG AIYVGNFRVV NRHLATHNDW ANLVWEDSSR 900
    DLLVSSTTAQ GCDTIARCDC QTGVYYCNSM RKHYPVSFSK PSLIYVEASE 950
    YYPARYQSHL MLAQGHSEPG DCGGILRCQH GVVGIVSTGG NGLVGFADVR 1000
    DLLWLDEEAM EQGVSDYIKG LGDAFGTGFT DAVSREVEAL KNYLIGSEGA 1050
    VEKILKNLIK LISALVIVIR SDYDMVTLTA TLALIGCHGS PWAWIKAKTA 1100
    SILGIPIAQK QSASWLKKFN DMANAAKGLE WVSNKISKFI DWLKEKIVPA 1150
    AREKVEFLNN LKQLPLLENQ ISNLEQSAAS QEDLEVMFGN VSYLAHFCRK 1200
    FQPLYATEAK RVYALEKRMN NYMQFKSKHR IEPVCLIIRG SPGTGKSLAT 1250
    GIIARAIADK YHSSVYSLPP DPDHFDGYKQ QVVTVMDDLC QNPDGKDMSL 1300
    FCQMVSTVDF IPPMASLEEK GVSFTSKFVI ASTNASNIIV PTVSDSDAIR 1350
    RRFYMDCDIE VTDSYKTDLG RLDAGRAAKL CSENNTANFK RCSPLVCGKA 1400
    IQLRDRKSKV RYSVDTVVSE LIREYSNRSA IGNTIEALFQ GPPKFRPIRI 1450
    SLEEKPAPDA ISDLLASVDS EEVRQYCRDQ GWIIPEAPTN VERHLNRAVL 1500
    VMQSIATVVA VVSLVYVIYK LFAGFQGAYS GAPKQVLKKP ALRTATVQGP 1550
    SLDFALSLLR RNIRQVQTDQ GHFTMLGVRD RLAVLPRHSQ PGKTIWIEHK 1600
    LVNVLDAVEL VDEQGVNLEL TLITLDTNEK FRDITKFIPE NISTASDATL 1650
    VINTEHMPSM FVPVGDVVQY GFLNLSGKPT HRTMMYNFPT KAGQCGGVVT 1700
    SVGKVVGIHI GGNGRQGFCA GLKRSYFASE QGEIQWVKPN KETGRLNING 1750
    PTRTKLEPSV FHDIFEGNKE PAVLHSKDPR LEVDFEQALF SKYVGNTLHE 1800
    PDEYIKEAAL HYANQLKQLE INTSQMSMEE ACYGTENLEA IDLHTSAGYP 1850
    YSALGIKKRD ILDPTTRDVS RMKFYMDKYG LDLPYSTYVK DELRSIDKIK 1900
    KGKSRLIEAS SLNDSVYLRM AFGHLYEAFH ANPGTITGSA VGCNPDTFWS 1950
    KLPILLPGSL FAFDYSGYDA SLSPVWFRAL ELVLREIGYS EEAISLIEGI 2000
    NHTHHVYRNK TYCVLGGMPS GCSGTSIFNS MINNIIIRAL LIKTFKGIDL 2050
    DELNMVAYGD DVLASYPFPI DCLELAKTGK EYGLTMTPAD KSPCFNEVNW 2100
    GNATFLKRGF LPDEQFPFLI HPTMPMREIH ESIRWTKDAR NTQDHVRSLC 2150
    LLAWHNGKQE YEKFVSTIRS VPVGRALAIP NYENLRRNWL ELF 2193
    Length:2,193
    Mass (Da):242,822
    Last modified:July 1, 2008 - v1
    Checksum:iB676993C284B76B9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU703812 Genomic RNA. Translation: ACD63039.1.
    EU703814 Genomic RNA. Translation: ACD63041.1.
    JX678875 Genomic RNA. Translation: AGH30716.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU703812 Genomic RNA. Translation: ACD63039.1 .
    EU703814 Genomic RNA. Translation: ACD63041.1 .
    JX678875 Genomic RNA. Translation: AGH30716.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3J22 electron microscopy 6.30 A 638-862 [» ]
    B 82-318 [» ]
    C 324-562 [» ]
    3J23 electron microscopy 9.20 A 638-862 [» ]
    B 82-318 [» ]
    C 324-562 [» ]
    3VBF X-ray 2.60 A 566-862 [» ]
    B 79-323 [» ]
    C 324-565 [» ]
    D 12-69 [» ]
    3VBH X-ray 2.30 A 566-862 [» ]
    B 79-323 [» ]
    C 324-565 [» ]
    D 12-69 [» ]
    3VBO X-ray 2.88 A 566-862 [» ]
    B 82-318 [» ]
    C 324-562 [» ]
    3VBR X-ray 3.80 A 638-862 [» ]
    B 82-318 [» ]
    C 324-562 [» ]
    3VBS X-ray 3.00 A 566-862 [» ]
    B 79-323 [» ]
    C 324-565 [» ]
    D 12-69 [» ]
    3VBU X-ray 4.00 A 638-862 [» ]
    B 82-318 [» ]
    C 324-562 [» ]
    4CDQ X-ray 2.65 A 566-862 [» ]
    B 70-323 [» ]
    C 324-565 [» ]
    D 1-69 [» ]
    4CDU X-ray 2.80 A 566-862 [» ]
    B 70-323 [» ]
    C 324-565 [» ]
    D 1-69 [» ]
    4CDW X-ray 2.80 A 566-862 [» ]
    B 70-323 [» ]
    C 324-565 [» ]
    D 1-69 [» ]
    4CDX X-ray 2.80 A 566-862 [» ]
    B 70-323 [» ]
    C 324-565 [» ]
    D 1-69 [» ]
    4CEW X-ray 2.75 A 566-862 [» ]
    B 70-323 [» ]
    C 324-565 [» ]
    D 1-69 [» ]
    4CEY X-ray 2.75 A 566-862 [» ]
    B 70-323 [» ]
    C 324-565 [» ]
    D 1-69 [» ]
    4IKA X-ray 2.70 D 1527-1548 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.20. 3 hits.
    3.40.50.300. 1 hit.
    4.10.80.10. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR014838. P3A.
    IPR000081. Peptidase_C3.
    IPR000199. Peptidase_C3A/C3B_picornavir.
    IPR003138. Pico_P1A.
    IPR002527. Pico_P2B.
    IPR001676. Picornavirus_capsid.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view ]
    Pfami PF08727. P3A. 1 hit.
    PF00548. Peptidase_C3. 1 hit.
    PF02226. Pico_P1A. 1 hit.
    PF00947. Pico_P2A. 1 hit.
    PF01552. Pico_P2B. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00073. Rhv. 3 hits.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    ProDomi PD001306. Peptidase_C3. 1 hit.
    PD649346. Pico_P2B. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF89043. SSF89043. 1 hit.
    PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An emerging recombinant human enterovirus 71 responsible for the 2008 outbreak of hand foot and mouth disease in Fuyang city of China."
      Zhang Y., Zhu Z., Yang W., Ren J., Tan X., Wang Y., Mao N., Xu S., Zhu S., Cui A., Zhang Y., Yan D., Li Q., Dong X., Zhang J., Zhao Y., Wan J., Feng Z.
      , Sun J., Wang S., Li D., Xu W.
      Virol. J. 7:94-94(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: EV71/Fuyang.Anhui.P.R.C/17.08/1Imported and EV71/Fuyang.Anhui.P.R.C/17.08/3Imported.
    2. Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 566-862; 79-323; 324-565 AND 12-69 IN COMPLEX WITH AMP.
    3. "Crystal structure of enterovirus 71 RNA-dependent RNA polymerase complexed with its protein primer VPg: implication for a trans mechanism of VPg uridylylation."
      Chen C., Wang Y., Shan C., Sun Y., Xu P., Zhou H., Yang C., Shi P.Y., Rao Z., Zhang B., Lou Z.
      J. Virol. 87:5755-5768(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 1527-1548.
    4. "Complete genome analysis of the c4 subgenotype strains of enterovirus 71: predominant recombination c4 viruses persistently circulating in china for 14 years."
      Zhang Y., Tan X., Cui A., Mao N., Xu S., Zhu Z., Zhou J., Shi J., Zhao Y., Wang X., Huang X., Zhu S., Zhang Y., Tang W., Ling H., Xu W.
      PLoS ONE 8:E56341-E56341(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: FY17.08-4/AH/CHN/2008Imported.
    5. "The enterovirus 71 A-particle forms a gateway to allow genome release: a cryoEM study of picornavirus uncoating."
      Shingler K.L., Yoder J.L., Carnegie M.S., Ashley R.E., Makhov A.M., Conway J.F., Hafenstein S.
      PLoS Pathog. 9:e1003240-e1003240(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (6.30 ANGSTROMS) OF 638-862; 82-318 AND 324-562.
    6. "More-powerful virus inhibitors from structure-based analysis of HEV71 capsid-binding molecules."
      De Colibus L., Wang X., Spyrou J.A., Kelly J., Ren J., Grimes J., Puerstinger G., Stonehouse N., Walter T.S., Hu Z., Wang J., Li X., Peng W., Rowlands D.J., Fry E.E., Rao Z., Stuart D.I.
      Nat. Struct. Mol. Biol. 21:282-288(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 566-862; 70-323; 324-565 AND 1-69.

    Entry informationi

    Entry nameiB2ZUN0_9ENTO
    AccessioniPrimary (citable) accession number: B2ZUN0
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 1, 2008
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3