Reviewed, UniProtKB/Swiss-Prot B2ZGJ1 (CLAT_DANRE)

Names and origin

Protein names

Recommended name:
    Choline O-acetyltransferase
      Short name=Choline acetylase
      Short name=CHOACTase
      Short name=ChAT
    EC=2.3.1.6

Gene names

Name:

chat

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Ref.1
Catalytic activity	Acetyl-CoA + choline = CoA + O-acetylcholine.
Sequence similarities	Belongs to the carnitine/choline acetyltransferase family.

Ontologies

Keywords
Biological process	Neurotransmitter biosynthesis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	locomotory behavior Ref.1 Inferred from mutant phenotype. Source: UniProtKB neurotransmitter biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	choline O-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	Choline O-acetyltransferase		PRO_0000351550
Regions
	Region	407 – 419	13	Coenzyme A binding By similarity
Sites
	Active site	329	1	Proton acceptor By similarity
	Binding site	445	1	Coenzyme A By similarity
	Binding site	545	1	Coenzyme A By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2ZGJ1-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 2379C23ADABC755A	FASTA	637	71,840
	10 20 30 40 50 60 MPVSKREQSK DTGDPCALPK LPIPPLKQTL DMYLTCMGHL VPEDQFRKTK AVVEKFGAPG 70 80 90 100 110 120 GVGETLQKKL LERSEQKANW VYDYWLEDMY LNNRLALPVN SSPVMVFHKQ NFKGQSDVLR 130 140 150 160 170 180 FAANLISGVL EYKALIDGRA LPVEHARGQL AGTPLCMDQY NKVFTSYRLP GTKTDTLVAQ 190 200 210 220 230 240 KSTVMPEPEH IIVACKNQFF VLDVMVNFRR LNEKDLYTQL ERIRKMADIE EERQPPIGLL 250 260 270 280 290 300 TSDGRTQWAE ARNILIKDST NRDSLDMIER CLCLVCLDEE TATELNDSNR ALLMLHGGGT 310 320 330 340 350 360 DKNGGNRWYD KPMQFVIGAD GCCGVVCEHS PFEGIVLVQC SEYLLRYMRG SPSKLVRAAS 370 380 390 400 410 420 MSELPAPRRL RWKCSPDIQT FLSASADRLQ KLVKNLDMNV HKFTGYGKEF IKRQKMSPDA 430 440 450 460 470 480 YVQVALQFTF YRCHGRLVPT YESASIRRFQ EGRVDNIRSS TPEALAFVKA MASGSKITDA 490 500 510 520 530 540 EKMELLWTAI KAQTNYTILA ITGMAIDNHL LGLREIAKEL KLEKPELFSD TTYATSIHFT 550 560 570 580 590 600 LSTSQVPTTE EMFCCYGPVV PNGYGACYNP QTDHILFCVS SFRECAETSS DLFVKTLEGC 610 620 630 LKEMQDLCRK CNTEVKPADS TQRMEGNPKV MKNGSKS

References

[1]	"Function of neuromuscular synapses in the zebrafish choline-acetyltransferase mutant bajan." Wang M., Wen H., Brehm P. J. Neurophysiol. 100:1995-2004(2008) [PubMed: 18684905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	EU660883 mRNA. Translation: ACD40042.1.
IPI	IPI00499667.
RefSeq	NP_001124191.1.
UniGene	Dr.117574
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2ZGJ1.
Genome annotation databases
Ensembl	ENSDART00000024225; ENSDARP00000025508; ENSDARG00000015854; Danio rerio. [Genome view]
GeneID	100170938.
KEGG	dre:100170938.
Organism-specific databases
CTD	100170938.
ZFIN	ZDB-GENE-080102-2. chat.
Gene expression databases
Bgee	B2ZGJ1.
Family and domain databases
InterPro	IPR000542. Carn_acyl_trans. [Graphical view]
PANTHER	PTHR22589. Carn_acyl_trans. 1 hit.
Pfam	PF00755. Carn_acyltransf. 1 hit. [Graphical view]
PROSITE	PS00439. ACYLTRANSF_C_1. 1 hit. PS00440. ACYLTRANSF_C_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLAT_DANRE

Accession

Primary (citable) accession number: B2ZGJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	July 1, 2008
Last modified:	October 13, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project