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Protein

Choline O-acetyltransferase

Gene

chat

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.1 Publication

Catalytic activityi

Acetyl-CoA + choline = CoA + O-acetylcholine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei329 – 3291Proton acceptorBy similarity
Binding sitei445 – 4451Coenzyme ABy similarity
Binding sitei545 – 5451Coenzyme ABy similarity

GO - Molecular functioni

  1. choline O-acetyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. locomotory behavior Source: UniProtKB
  2. neurotransmitter biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Neurotransmitter biosynthesis

Enzyme and pathway databases

ReactomeiREACT_174660. Synthesis of PC.
REACT_238198. Acetylcholine Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline O-acetyltransferase (EC:2.3.1.6)
Short name:
CHOACTase
Short name:
ChAT
Short name:
Choline acetylase
Gene namesi
Name:chat
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 13

Organism-specific databases

ZFINiZDB-GENE-080102-2. chata.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 637637Choline O-acetyltransferasePRO_0000351550Add
BLAST

Expressioni

Gene expression databases

BgeeiB2ZGJ1.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000025508.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni407 – 41913Coenzyme A bindingBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG70127.
GeneTreeiENSGT00760000119220.
HOGENOMiHOG000233845.
HOVERGENiHBG107717.
InParanoidiB2ZGJ1.
KOiK00623.
OMAiHVIVACC.
OrthoDBiEOG7WHH90.
PhylomeDBiB2ZGJ1.
TreeFamiTF313836.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2ZGJ1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPVSKREQSK DTGDPCALPK LPIPPLKQTL DMYLTCMGHL VPEDQFRKTK
60 70 80 90 100
AVVEKFGAPG GVGETLQKKL LERSEQKANW VYDYWLEDMY LNNRLALPVN
110 120 130 140 150
SSPVMVFHKQ NFKGQSDVLR FAANLISGVL EYKALIDGRA LPVEHARGQL
160 170 180 190 200
AGTPLCMDQY NKVFTSYRLP GTKTDTLVAQ KSTVMPEPEH IIVACKNQFF
210 220 230 240 250
VLDVMVNFRR LNEKDLYTQL ERIRKMADIE EERQPPIGLL TSDGRTQWAE
260 270 280 290 300
ARNILIKDST NRDSLDMIER CLCLVCLDEE TATELNDSNR ALLMLHGGGT
310 320 330 340 350
DKNGGNRWYD KPMQFVIGAD GCCGVVCEHS PFEGIVLVQC SEYLLRYMRG
360 370 380 390 400
SPSKLVRAAS MSELPAPRRL RWKCSPDIQT FLSASADRLQ KLVKNLDMNV
410 420 430 440 450
HKFTGYGKEF IKRQKMSPDA YVQVALQFTF YRCHGRLVPT YESASIRRFQ
460 470 480 490 500
EGRVDNIRSS TPEALAFVKA MASGSKITDA EKMELLWTAI KAQTNYTILA
510 520 530 540 550
ITGMAIDNHL LGLREIAKEL KLEKPELFSD TTYATSIHFT LSTSQVPTTE
560 570 580 590 600
EMFCCYGPVV PNGYGACYNP QTDHILFCVS SFRECAETSS DLFVKTLEGC
610 620 630
LKEMQDLCRK CNTEVKPADS TQRMEGNPKV MKNGSKS
Length:637
Mass (Da):71,840
Last modified:July 1, 2008 - v1
Checksum:i2379C23ADABC755A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU660883 mRNA. Translation: ACD40042.1.
RefSeqiNP_001124191.1. NM_001130719.1.
XP_005156786.1. XM_005156729.2.
XP_009305555.1. XM_009307280.1.
UniGeneiDr.117574.

Genome annotation databases

EnsembliENSDART00000024225; ENSDARP00000025508; ENSDARG00000015854.
ENSDART00000150228; ENSDARP00000125038; ENSDARG00000015854.
GeneIDi100170938.
KEGGidre:100170938.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU660883 mRNA. Translation: ACD40042.1.
RefSeqiNP_001124191.1. NM_001130719.1.
XP_005156786.1. XM_005156729.2.
XP_009305555.1. XM_009307280.1.
UniGeneiDr.117574.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000025508.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000024225; ENSDARP00000025508; ENSDARG00000015854.
ENSDART00000150228; ENSDARP00000125038; ENSDARG00000015854.
GeneIDi100170938.
KEGGidre:100170938.

Organism-specific databases

CTDi100170938.
ZFINiZDB-GENE-080102-2. chata.

Phylogenomic databases

eggNOGiNOG70127.
GeneTreeiENSGT00760000119220.
HOGENOMiHOG000233845.
HOVERGENiHBG107717.
InParanoidiB2ZGJ1.
KOiK00623.
OMAiHVIVACC.
OrthoDBiEOG7WHH90.
PhylomeDBiB2ZGJ1.
TreeFamiTF313836.

Enzyme and pathway databases

ReactomeiREACT_174660. Synthesis of PC.
REACT_238198. Acetylcholine Neurotransmitter Release Cycle.

Miscellaneous databases

NextBioi20795505.
PROiB2ZGJ1.

Gene expression databases

BgeeiB2ZGJ1.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Function of neuromuscular synapses in the zebrafish choline-acetyltransferase mutant bajan."
    Wang M., Wen H., Brehm P.
    J. Neurophysiol. 100:1995-2004(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.

Entry informationi

Entry nameiCLAT_DANRE
AccessioniPrimary (citable) accession number: B2ZGJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: July 1, 2008
Last modified: January 7, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.