Reviewed, UniProtKB/Swiss-Prot B2ZDY2 (L_WWAVU)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase
    EC=2.7.7.48
Alternative name(s):
    Protein L

Gene names

Name:

L

Organism

Whitewater arroyo virus (isolate Rat/United States/AV 9310135/1995) (WWAV)

Taxonomic identifier

46919 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses

Virus host

Neotoma (wood rats) [TaxID: 42407]

Protein attributes

Sequence length	2219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2219	2219	RNA-directed RNA polymerase		PRO_0000361648
Regions
	Domain	1170 – 1367	198	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	B2ZDY2-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 91D73267930FBB7D	FASTA	2,219	255,596
	10 20 30 40 50 60 MVDCNDRINE LKDLIRKWVP DEEAYTEQKT TFLSQVNPSS IITEGLKLLS MLIEIDSCLK 70 80 90 100 110 120 HGCVFNRNKT VNQILKDHRI IGPTLPDVVP DGYRVSGSTI ILLETFVRVN QESFEQKYKH 130 140 150 160 170 180 DFEKLIQLSK DLSKCGLILV PVIDGRSNYY VDRFPDWVVE RIRWLLLKLM DNVKDTGERI 190 200 210 220 230 240 EELEYNRLIS SLSSMENQNL GLESLKALRE EGLDYKTKLM EAIRDGIRPN LTASECRIGI 250 260 270 280 290 300 AKVYDQFCLL RDSGQYQNVY CRTSRSEMIE WLKDHKLTSL INGSEGTFFN NERCGFCQNH 310 320 330 340 350 360 MLRVIAELVH SKRVSSNYTP TNDKEISRHK KLLSDCNKIK GLKVLNTRRH TLLCLDVIVL 370 380 390 400 410 420 NSLIEMIKLK INSSQFLINN HFKSVNDRLL SVDLIINKLE KKLLKQPDWL GNVKGKLSKR 430 440 450 460 470 480 IKPYNLDYVI TWLKELDYEF WYEFKFEREH SARYEKPTLR YKRQSERVCY QVEFGTDKIF 490 500 510 520 530 540 NEEVFLEYLD ALSSLSLGMM NSMKTSSATK LVINDEKSFY GTVQCEECYF QDLDSSYNSI 550 560 570 580 590 600 LLYQKTGEKS RCYGLMLNDD ELSDVYRVGQ SFYADPKRFF LPIMSSNVIL KTCEEMLSWL 610 620 630 640 650 660 DWLTNEETNM IRTKLFTLVI NVLTVPSKRV QIYLQGFRYF IMAFVNEFHF KRLNEKLSVQ 670 680 690 700 710 720 ALTSAEHHVF VLMDELVVCL LEEALEENMA KIFKFVLNLS YLCHFITKET PDRLTDQIKC 730 740 750 760 770 780 FEKFLEPKLQ FGSAFVNLDS SPSLPKEIED KFVKDINNLF SKDLHTNDLE SPGLSKEILS 790 800 810 820 830 840 LCTSCFNCGM LTVGKVLQND PQSPSFSSTA LDISSNKSVV VPKLDEVGEI ITHYDYQNLL 850 860 870 880 890 900 SSVVVEMAQS FKDKLRYKLD RKSVQYAIYR RLTNMVLTKN LNEKNHINEN CEDFEEFLDE 910 920 930 940 950 960 DTCRLISDIE SNVLECLSNM ATPPVRKVIK GQEITQRYEG PDLLGRLWSR ELMGPILAET 970 980 990 1000 1010 1020 SLHEVKDFDP SIFSFETYQE LCELIFNSEL KEQFFLDDVL RFCPLELLVK NLTTKNFIEK 1030 1040 1050 1060 1070 1080 DFFECFKYIL ISAGFDNRVG RYDHRSRSRL GFKDAAYHVK EKSRISLRES NSEAVSKRLD 1090 1100 1110 1120 1130 1140 KSFFTNSSLR NLCFYSEESP TFQSTVSSST GKLKFGLSYK EQVGSNRELY VGDLNTKLTS 1150 1160 1170 1180 1190 1200 RLIEDYFESI TSESKFSCLN NDSEFEKAIL DMKSVVRLSG LAVSLDHSKW GPYMSPAIFN 1210 1220 1230 1240 1250 1260 SLFTSLDLQL RDGSLIDKSP IENLLNWHLH KLVEVPYNVI EAYLKGYTKR GLGLMDKMSS 1270 1280 1290 1300 1310 1320 TVCEDFIFNW FAKGQVPSHI SSVLDMGQGI LHNTSDYYGL VTEQFIMLCL ELCFDVRMTA 1330 1340 1350 1360 1370 1380 YTSSDDEIML SNSHSLKDKS DESLDIQKCG ELLEFHYYLS SKLNKFVSPK TVAGSFASEF 1390 1400 1410 1420 1430 1440 KSRFFIWSQE VPLLTKFVAA ALHNVKAKSP HQLAETVDTI LDQCVANGVS IKIIKEISKR 1450 1460 1470 1480 1490 1500 TNRLISYSGH PIDPFLCVVE TDLKDWVDGS RGYRLQRSIE SIIADDKQLS IIRNSCKKLF 1510 1520 1530 1540 1550 1560 YKIRSGDIQE EYLVNALQSS PDDCLRQMLR ITEVDDQTIE KLIEIRWLNL RAFGDLRLVL 1570 1580 1590 1600 1610 1620 RTKIMSGTRI LDREEVPSLI RSVQSKLSKN FVRGAKKIVT DAINKSAFQS SVCSGFIGVC 1630 1640 1650 1660 1670 1680 KSMGSKCVRD GSGGFVYIKS LLSEVVCHHT CETCKPRFSV YCKSALERIS KYSRSLLWDY 1690 1700 1710 1720 1730 1740 FSLVFTNACE LGNWVFSCVE TPKKIPSVVN PNFFWCVKPG SHTELEDKVN MNHVLYSIKR 1750 1760 1770 1780 1790 1800 NFPDLFDEHI APFLSDLSSL KISWVQRIRF LDLCVAMDMS SECLGVISHI MRRKREESYI 1810 1820 1830 1840 1850 1860 VKQNELSLAH MRDSTPLEGG FQLNSLEICR NFLYQIVFES MLHPVLLTTS QFKKYFWYGE 1870 1880 1890 1900 1910 1920 VELLPNDADH DLGQLTQFIM DCKTLNVSRC MSLDDLDVGY VHSKITLSDV FINLSSFIHL 1930 1940 1950 1960 1970 1980 LDWGNLCDYE SFDKIILESG LEQVPIEIGI VVSHVRRSFK FKYDRKTNYH IKCKIIIRKS 1990 2000 2010 2020 2030 2040 ELIMSKVNGV DILEIEVSEI ECFVSGSQGH HISLDGVGLI PLHPLFSGKE LIDFNKLLAD 2050 2060 2070 2080 2090 2100 QSIEFKQVSS VFQKVKLDFK QHVKELRNKF SYKFQGPEQG LSPLHLYRGQ IIERDTIVSR 2110 2120 2130 2140 2150 2160 LDVPVTSKSV FLALEALDAA DHTPFLKSLH TYMKTRMSKS NPCFIRMTQE DLCLLIESYE 2170 2180 2190 2200 2210 VAFANILKSE SDWVEFGDFA LCFSNSLNCI MIADDGGQFK LKGRKCRSAS TNPRPLEIE

References

[1]	"Phylogeny of the genus Arenavirus." Charrel R.N., de Lamballerie X., Emonet S. Curr. Opin. Microbiol. 11:362-368(2008) [PubMed: 18602020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: AV 9310135.

Cross-references

Sequence databases
EMBLGenBankDDBJ	EU646186 Genomic RNA. Translation: ACD03600.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_WWAVU

Accession

Primary (citable) accession number: B2ZDY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	July 1, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)