B2WAQ3 (LCPS_PYRTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional lycopene cyclase/phytoene synthase

Including the following 2 domains:

1. Lycopene beta-cyclase
   EC=5.5.1.19
   Alternative name(s):
   Lycopene cyclase
2. Phytoene synthase
   EC=2.5.1.32

Gene names

ORF Names:

PTRG_07366

Organism

Pyrenophora tritici-repentis (strain Pt-1C-BFP) (Wheat tan spot fungus) (Drechslera tritici-repentis) [Complete proteome]

Taxonomic identifier

426418 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › dothideomyceta › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporineae › Pleosporaceae › Pyrenophora › Pyrenophora tritici-repentis

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase) By similarity.
Catalytic activity	Carotenoid psi-end group = carotenoid beta-end group. 2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; beta-carotene biosynthesis. Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	In the N-terminal section; belongs to the lycopene beta-cyclase family. In the C-terminal section; belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Isomerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC intramolecular lyase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 583	583	Bifunctional lycopene cyclase/phytoene synthase		PRO_0000409242
Regions
	Transmembrane	3 – 23	21	Helical; Potential
	Transmembrane	35 – 55	21	Helical; Potential
	Transmembrane	75 – 97	23	Helical; Potential
	Transmembrane	120 – 140	21	Helical; Potential
	Transmembrane	151 – 171	21	Helical; Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Transmembrane	221 – 241	21	Helical; Potential
	Region	1 – 243	243	Lycopene beta-cyclase
	Region	250 – 583	334	Phytoene synthase

Sequences

	Sequence		Length	Mass (Da)
	B2WAQ3 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: F0851DA16FAE327E	FASTA	583	66,281
	10 20 30 40 50 60 MGFDYALVHL KYTIPPAVLL TWLYRPFFTK LDVYKVGYLV SIAVASTIPW DSYLIRTGIW 70 80 90 100 110 120 SYPTHVIIGP KLCDIPLEEV FFFIIQTYNT SLLYLLLSRP TFQPVYLNTE RGAARRQWRY 130 140 150 160 170 180 MRLAGQVFFL ALIAWGWRCI RHGGLGTYTG LILVWAGPFL LMLWSLAYQF ILALPVTNTA 190 200 210 220 230 240 LPIFLPTLYL WVVDTLALRR GTWVISTGTK YGLHLWDGLE IEEALFFLAT NALIVFGQLA 250 260 270 280 290 300 FDNALAVLYT FPHLFTGPSL LPSPVLLMRA LLTPCSKYHD ARIKGLDEAV NRLKRKSRSF 310 320 330 340 350 360 YLASATFPGP LRADLLLLYS FCRVADDLVD NASDADEARA WIAKMRKFLN NVYSDKLPQS 370 380 390 400 410 420 VVHSQICDDF PPSTQSALLQ LPATKLSPQP LEDLLHGFEM DLAFQQGPII RTMEDLRVYS 430 440 450 460 470 480 ERVAGTVAQM CIQLIFYWYP STLDTEEKNV IVAAGNSMGV ALQYVNIARD IEVDAQIGRV 490 500 510 520 530 540 YLPLNWLSEA GLSYDDVLKK PNQAQIQTLR KHLLNHAFSV YEKAKDSIER LPIEARGPIR 550 560 570 580 VAVESYMEIG RILRSEQYQV KAGRATVPKS RRIMVAWRTL NSK

References

[1]

"Genome sequence of Pyrenophora tritici-repentis." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., MacCallum I.A., Young S.K., LaButti K., DeCaprio D. , Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., White J., Yandava C., Kodira C.D., Guigo R., Borodovsky M., Zeng Q., O'Leary S., Alvarado L., Pandelova I., Ciuffetti L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pt-1C-BFP.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS231621 Genomic DNA. Translation: EDU50285.1.
RefSeq	XP_001937698.1. XM_001937663.1.
3D structure databases
ProteinModelPortal	B2WAQ3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EDU50285; EDU50285; PTRG_07366.
GeneID	6345639.
Phylogenomic databases
OrthoDB	EOG4HQHST.
Enzyme and pathway databases
UniPathway	UPA00799; UER00773. UPA00802.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR017825. Lycopene_cyclase_dom. IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
TIGRFAMs	TIGR03462. CarR_dom_SF. 2 hits.
PROSITE	PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCPS_PYRTR

Accession

Primary (citable) accession number: B2WAQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	July 1, 2008
Last modified:	May 29, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program