B2VJP2 (IOLE_ERWT9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inosose dehydratase
EC=4.2.1.44

Alternative name(s):
2-keto-myo-inositol dehydratase
Short name=2KMI dehydratase

Gene names

Name:	iolE
Ordered Locus Names:	ETA_32860

Organism

Erwinia tasmaniensis (strain DSM 17950 / Et1/99) [Complete proteome] [HAMAP]

Taxonomic identifier

338565 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Erwinia

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the dehydration of inosose (2-keto-myo-inositol, 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi-inositol) By similarity. HAMAP-Rule MF_01672
Catalytic activity	2,4,6/3,5-pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H2O. HAMAP-Rule MF_01672
Cofactor	Glutathione By similarity. Cobalt or manganese By similarity.
Sequence similarities	Belongs to the IolE/MocC family.

Ontologies

Keywords
Ligand	Cobalt Manganese
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	manganese ion binding Inferred from electronic annotation. Source: HAMAP myo-inosose-2 dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Inosose dehydratase HAMAP-Rule MF_01672		PRO_1000187325

Sequences

	Sequence		Length	Mass (Da)
	B2VJP2 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 11EFC63015268470	FASTA	298	33,443
	10 20 30 40 50 60 MDKNKVKLAI APIGWTNDDM PELGKENSFQ QIVSEMALAG FTGSEVGSKY PRDPAVLKPM 70 80 90 100 110 120 LDIRGLEIVN AWFSTFFANG DREKTLDEFI NHRDFLHAMG AKVIGCSEQS LSIQGTAKAV 130 140 150 160 170 180 LEEKPHFSDE QWRLTVEGYN QLAKLAAEKG MTVGLHHHMG TAIQTCAEVD RFMAQTHDDV 190 200 210 220 230 240 YLLFDTGHAY YSEGSQQAML AMLEKHLARI NHVHLKDVRD EVVAEVKAQR LSFLEGVKRG 250 260 270 280 290 TFTVPGDGVI DFDPVFKILD DSGYRGWMVV EAEQDPARAN PFEYALKARR YIRQHAGL

References

[1]

"The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia." Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K. Environ. Microbiol. 10:2211-2222(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17950 / Et1/99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU468135 Genomic DNA. Translation: CAO98332.1.
RefSeq	YP_001909193.1. NC_010694.1.
3D structure databases
ProteinModelPortal	B2VJP2.
ModBase	Search...
Protein-protein interaction databases
STRING	465817.ETA_32860.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAO98332; CAO98332; ETA_32860.
GeneID	6298737.
KEGG	eta:ETA_32860.
PATRIC	20432497. VBIErwTas9546_3375.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1082.
HOGENOM	HOG000239720.
KO	K03335.
OMA	DMPDLGK.
ProtClustDB	CLSK957433.
Enzyme and pathway databases
BioCyc	ETAS465817:GI36-2398-MONOMER.
Family and domain databases
Gene3D	3.20.20.150. 1 hit.
HAMAP	MF_01672. IolE.
InterPro	IPR023952. IolE. IPR013022. Xyl_isomerase-like_TIM-brl. [Graphical view]
Pfam	PF01261. AP_endonuc_2. 1 hit. [Graphical view]
SUPFAM	SSF51658. Xyl_isomerase-like_TIM-brl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

IOLE_ERWT9

Accession

Primary (citable) accession number: B2VJP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program