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Protein

Ribosomal RNA small subunit methyltransferase F

Gene

rsmF

Organism
Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei150 – 1501S-adenosyl-L-methionineUniRule annotation
Binding sitei177 – 1771S-adenosyl-L-methionineUniRule annotation
Binding sitei195 – 1951S-adenosyl-L-methionineUniRule annotation
Active sitei248 – 2481NucleophileUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciETAS465817:GI36-1569-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase FUniRule annotation (EC:2.1.1.178UniRule annotation)
Alternative name(s):
16S rRNA m5C1407 methyltransferaseUniRule annotation
rRNA (cytosine-C(5)-)-methyltransferase RsmFUniRule annotation
Gene namesi
Name:rsmFUniRule annotation
Ordered Locus Names:ETA_15110
OrganismiErwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)
Taxonomic identifieri465817 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia
Proteomesi
  • UP000001726 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478Ribosomal RNA small subunit methyltransferase FPRO_1000185643Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi465817.ETA_15110.

Structurei

3D structure databases

ProteinModelPortaliB2VJ83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1327S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
COG3270. LUCA.
HOGENOMiHOG000218115.
KOiK11392.
OMAiWCAEGFW.
OrthoDBiEOG6091D0.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01579. 16SrRNA_methyltr_F.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR027391. Nol1_Nop2_Fmu_2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF13636. Methyltranf_PUA. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2VJ83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHTSRVFFP PAFVEQMQQL LPDADEFQRF ITSSQLPLRR SLRVNTLKIS
60 70 80 90 100
VDDFLTLVEP YQWALTPIPW CQEGFWISRD DADTLPLGST AEHLAGLFYI
110 120 130 140 150
QEASSMLPVS ALFDGGDMPQ RVMDMAAAPG SKTTQIAAHM GNRGAILANE
160 170 180 190 200
YSASRVKVLH ANLSRCGVSN TAMTHFDGRV FGPALPECFD AVLLDAPCSG
210 220 230 240 250
EGVVRKDADA LRNWSQSSTE EIAATQQALI NSAFHALRPG GTLVYSTCTL
260 270 280 290 300
NTRENQQVVN GLLEQYPDAV KVEPLDTLFM GAERATTAEG YLHVFPHIFD
310 320 330 340 350
SEGFFVARLR KVASVPPMPQ PGYKVGKPPF TPLNRVQQQE VIAAAASVGL
360 370 380 390 400
RWDTELKLWQ RDKELWLFPS EIEPLLGRVR FSRIGLKLAE TFPKGFRWQH
410 420 430 440 450
HAAVALADAA SANAFELNSN EAEEWYRGRD IYPERDLPAS ELIICYQQRP
460 470
IGIAKKVGSR IKNNYPRELV RDGKLFTL
Length:478
Mass (Da):53,266
Last modified:July 1, 2008 - v1
Checksum:i23752FAD0B2951CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU468135 Genomic DNA. Translation: CAO96557.1.

Genome annotation databases

EnsemblBacteriaiCAO96557; CAO96557; ETA_15110.
KEGGieta:ETA_15110.
PATRICi20428818. VBIErwTas9546_1582.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU468135 Genomic DNA. Translation: CAO96557.1.

3D structure databases

ProteinModelPortaliB2VJ83.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi465817.ETA_15110.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAO96557; CAO96557; ETA_15110.
KEGGieta:ETA_15110.
PATRICi20428818. VBIErwTas9546_1582.

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
COG3270. LUCA.
HOGENOMiHOG000218115.
KOiK11392.
OMAiWCAEGFW.
OrthoDBiEOG6091D0.

Enzyme and pathway databases

BioCyciETAS465817:GI36-1569-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01579. 16SrRNA_methyltr_F.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR027391. Nol1_Nop2_Fmu_2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF13636. Methyltranf_PUA. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia."
    Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K.
    Environ. Microbiol. 10:2211-2222(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17950 / CIP 109463 / Et1/99.

Entry informationi

Entry nameiRSMF_ERWT9
AccessioniPrimary (citable) accession number: B2VJ83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 1, 2008
Last modified: June 8, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.