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Protein

3-ketoacyl-CoA thiolase

Gene

fadI

Organism
Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed.UniRule annotation

Catalytic activityi

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei99Acyl-thioester intermediateUniRule annotation1
Active sitei392Proton acceptorUniRule annotation1
Active sitei422Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA thiolaseUniRule annotation (EC:2.3.1.16UniRule annotation)
Alternative name(s):
ACSsUniRule annotation
Acetyl-CoA acyltransferaseUniRule annotation
Acyl-CoA ligaseUniRule annotation
Beta-ketothiolaseUniRule annotation
Fatty acid oxidation complex subunit betaUniRule annotation
Gene namesi
Name:fadIUniRule annotation
Ordered Locus Names:ETA_11490
OrganismiErwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)
Taxonomic identifieri465817 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeErwinia
Proteomesi
  • UP000001726 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001859681 – 4373-ketoacyl-CoA thiolaseAdd BLAST437

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi465817.ETA_11490.

Structurei

3D structure databases

ProteinModelPortaliB2VJ10.
SMRiB2VJ10.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHU. Bacteria.
COG0183. LUCA.
HOGENOMiHOG000012240.
KOiK00632.
OrthoDBiPOG091H065K.

Family and domain databases

CDDicd00751. thiolase. 1 hit.
Gene3Di3.40.47.10. 2 hits.
HAMAPiMF_01618. FadI. 1 hit.
InterProiView protein in InterPro
IPR012806. Ac-CoA_C-AcTrfase_FadI.
IPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
PANTHERiPTHR18919:SF128. PTHR18919:SF128. 1 hit.
PfamiView protein in Pfam
PF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
TIGR02446. FadI. 1 hit.
PROSITEiView protein in PROSITE
PS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

B2VJ10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKALPLLTR QGERIAITHG LRTPFARQAT AFHGIPALEL GRMVVSELLA
60 70 80 90 100
RSEISPDVIE QLVFGQVVQM PEAPNIAREI VLASALSVHT DAWSVSRACA
110 120 130 140 150
TSFQAVANVA ESLMIGHIQA GIAGGADSSS VLPIGVSKKL ARLLVDASKT
160 170 180 190 200
RSLAQKLKLF SGLRPRDLLP VAPAVAEYST GLRMGDSAEQ MAKNHGITRE
210 220 230 240 250
QQDALALRSH QRAAHAWQQG LLNDEVMTAC VPPWEQPFEQ DNNVRAESKM
260 270 280 290 300
QDYARLRPAF DRRHGTVTAA NSTPLTDGAA AVILMTASRA RELGIAPLGF
310 320 330 340 350
LRSYAFSAID VRQDMLLGPS YASPLALDRA GITLADLSLI DMHEAFAAQT
360 370 380 390 400
LANLKMFADE RFAREVLDRP RALGEVDMER FNVLGGSIAY GHPFAATGAR
410 420 430
MITQTLNELR RRGGGLGLVT ACAAGGLGAA MVLEVDS
Length:437
Mass (Da):46,832
Last modified:July 1, 2008 - v1
Checksum:i706E3783304700A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU468135 Genomic DNA. Translation: CAO96195.1.
RefSeqiWP_012440892.1. NC_010694.1.

Genome annotation databases

EnsemblBacteriaiCAO96195; CAO96195; ETA_11490.
KEGGieta:ETA_11490.

Similar proteinsi

Entry informationi

Entry nameiFADI_ERWT9
AccessioniPrimary (citable) accession number: B2VJ10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 1, 2008
Last modified: August 30, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families