B2VHX9 (LPXB_ERWT9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipid-A-disaccharide synthase
EC=2.4.1.182

Gene names

Name:	lpxB
Ordered Locus Names:	ETA_09030

Organism

Erwinia tasmaniensis (strain DSM 17950 / Et1/99) [Complete proteome] [HAMAP]

Taxonomic identifier

338565 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Erwinia

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP-Rule MF_00392
Catalytic activity	UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. HAMAP-Rule MF_00392
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 5/6. HAMAP-Rule MF_00392
Sequence similarities	Belongs to the LpxB family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid biosynthesis Lipid metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	lipid-A-disaccharide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Lipid-A-disaccharide synthase HAMAP-Rule MF_00392		PRO_1000191473

Sequences

	Sequence		Length	Mass (Da)
	B2VHX9 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 4AA2D02AC4D91D04	FASTA	381	42,433
	10 20 30 40 50 60 MPKHPLTIAL VAGETSGDIL GAGLIRALKE KHPDARFVGV AGPLMQSEGC EAWYEMEELA 70 80 90 100 110 120 VMGIVEVLGR LRRLLHIRRD LTRRFTALKP DVFVGIDAPD FNITLEGRLK QQGIRTIHYV 130 140 150 160 170 180 SPSVWAWRQK RVFKIGRATD LVLAFLPFEK AFYDRFNVPC RFIGHTMADA MPIVPDKQAA 190 200 210 220 230 240 RRELGIAPQA LCLALLPGSR SAEVEMLSAD FLKTAMLLRE KYPQLEIVVP LVNPRRRAQF 250 260 270 280 290 300 EAIKAEVAAD LPMHLLDGKG REAMLASDAA LLASGTAALE CMLAKCPMVV GYRMKPFTFW 310 320 330 340 350 360 LAKRLVKTDY VSLPNLLAGR ELVPELLQDE CQPQRLAAAL EPLLAQGETR DTLLATFAEL 370 380 HHQIRWNADE QAAAAVLELC R

References

[1]

"The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia." Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K. Environ. Microbiol. 10:2211-2222(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17950 / Et1/99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU468135 Genomic DNA. Translation: CAO95949.1.
RefSeq	YP_001906847.1. NC_010694.1.
3D structure databases
ProteinModelPortal	B2VHX9.
ModBase	Search...
Protein-protein interaction databases
STRING	465817.ETA_09030.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAO95949; CAO95949; ETA_09030.
GeneID	6299270.
KEGG	eta:ETA_09030.
PATRIC	20427514. VBIErwTas9546_0948.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0763.
HOGENOM	HOG000018003.
KO	K00748.
OMA	PTVWAWR.
ProtClustDB	PRK00025.
Enzyme and pathway databases
BioCyc	ETAS465817:GI36-2460-MONOMER.
UniPathway	UPA00359; UER00481.
Family and domain databases
HAMAP	MF_00392. LpxB.
InterPro	IPR003835. Glyco_trans_19. [Graphical view]
Pfam	PF02684. LpxB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00215. lpxB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXB_ERWT9

Accession

Primary (citable) accession number: B2VHX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program