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B2VHN8 (BGAL_ERWT9) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:ETA_25680
OrganismErwinia tasmaniensis (strain DSM 17950 / Et1/99) [Complete proteome] [HAMAP]
Taxonomic identifier338565 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia

Protein attributes

Sequence length1026 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10261026Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000366987

Regions

Region539 – 5424Substrate binding By similarity

Sites

Active site4631Proton donor By similarity
Active site5391Nucleophile By similarity
Metal binding2031Sodium By similarity
Metal binding4181Magnesium 1 By similarity
Metal binding4201Magnesium 1 By similarity
Metal binding4631Magnesium 1 By similarity
Metal binding5991Magnesium 2 By similarity
Metal binding6031Sodium; via carbonyl oxygen By similarity
Metal binding6061Sodium By similarity
Binding site1041Substrate By similarity
Binding site2031Substrate By similarity
Binding site4631Substrate By similarity
Binding site6061Substrate By similarity
Binding site10021Substrate By similarity
Site3591Transition state stabilizer By similarity
Site3931Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
B2VHN8 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 63A6F9463A3FB870

FASTA1,026116,920
        10         20         30         40         50         60 
MAKTDPLTLQ QLLAQRHWEN PALTQLNRLP AHTPLASWRN ADAARGDLAS PSQRLLDGEW 

        70         80         90        100        110        120 
GFSYFDRPQA VPDAWINGDL PQVGRLSVPS NWQLSGYDAP IYTNVQYPIP TDPPRVPDDN 

       130        140        150        160        170        180 
PTGCYSLTFH CQPAWLQSGQ TRIIFDGVNS AFYLWCNGEF IGYSQDSRLP AEFDLSQRLL 

       190        200        210        220        230        240 
EGENRIAVMV LRWCDGSYLE DQDMWRMSGI FRSVSLLHKP HVRLDDIHID TRLSPEYRSA 

       250        260        270        280        290        300 
QLRVLALCSL TDASAYQLKV TLWRDDTLIA QHQQPFGTPV VDERGRYLDR TRLSLQIDQP 

       310        320        330        340        350        360 
LLWNAETPHL YRAVIALLDA DGTLIEAEAC DVGFRQVEVS GGLLKLNGKA LLIRGTNRHE 

       370        380        390        400        410        420 
HHPDRGQVMD EPAMIADILL MKQHNFNAVR CSHYPNHPLW YRLCDRYGLY VVDEANIETH 

       430        440        450        460        470        480 
GMQPMNRLAD DPSWFSAFSE RVTRMVQRDR NHACIIIWSL GNESGHGSTH DALYGWIKSD 

       490        500        510        520        530        540 
DPSRPVQYEG GGADTAASDI ICPMYARVDR DQPFEAVPKW SIKKWIALPE ETRPLILCEY 

       550        560        570        580        590        600 
AHAMGNSLGG FSRYWQAFRQ YPPLQGGFVW DWADQNLTRQ AADGSSWQAY GGDFGDMPND 

       610        620        630        640        650        660 
RQFCMNGLVF ADRSPHPALF EAKRAQQFFQ FQLENTSPIT LGITSEYLFR HSDNERLCWR 

       670        680        690        700        710        720 
IEHHGEQVAG GQITLNLPPE GTVSLMLGEL PSLAGELWLR VEVIQPAATA WSPANHRVAW 

       730        740        750        760        770        780 
DGWQLPAALS LPKPDVPGEQ PRLHPQSQLI EVVQAGQRWQ FCRQSGELIQ WLQADKPQLL 

       790        800        810        820        830        840 
SPLRDLFVRA PLDNDIGISE ANRIDPHAWA ERWQRAGYYR LESQLLRLQT DILNDGVQIR 

       850        860        870        880        890        900 
SEQAWLADGE PRFLSRKCYR INRQGEMLLE VEVDIAAGLP EPARIGLHCQ LAEVAEEVCW 

       910        920        930        940        950        960 
LGLGPHENYP DRRLAAEFSR WQLPLGALST PYVFPCENGL RGGTRELTFG HWHIRGDFHF 

       970        980        990       1000       1010       1020 
SLSRHSVEQL RKTSHRHLLR DEAGCWLTLD GFHMGVGGDD SWSPSVDEEF LLRARQYRYR 


LILTRG 

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References

[1]"The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia."
Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K.
Environ. Microbiol. 10:2211-2222(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17950 / Et1/99.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU468135 Genomic DNA. Translation: CAO97614.1.
RefSeqYP_001908490.1. NC_010694.1.

3D structure databases

ProteinModelPortalB2VHN8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING465817.ETA_25680.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAO97614; CAO97614; ETA_25680.
GeneID6299686.
KEGGeta:ETA_25680.
PATRIC20431029. VBIErwTas9546_2664.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMANPPFVPK.
OrthoDBEOG6XWV0T.

Enzyme and pathway databases

BioCycETAS465817:GI36-2651-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_ERWT9
AccessionPrimary (citable) accession number: B2VHN8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries