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Protein

Beta-galactosidase

Gene

lacZ

Organism
Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104SubstrateUniRule annotation1
Metal bindingi203SodiumUniRule annotation1
Binding sitei203SubstrateUniRule annotation1
Sitei359Transition state stabilizerUniRule annotation1
Sitei393Transition state stabilizerUniRule annotation1
Metal bindingi418Magnesium 1UniRule annotation1
Metal bindingi420Magnesium 1UniRule annotation1
Active sitei463Proton donorUniRule annotation1
Metal bindingi463Magnesium 1UniRule annotation1
Binding sitei463SubstrateUniRule annotation1
Active sitei539NucleophileUniRule annotation1
Metal bindingi599Magnesium 2UniRule annotation1
Metal bindingi603Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi606SodiumUniRule annotation1
Binding sitei606SubstrateUniRule annotation1
Binding sitei1002SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandMagnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2 Glycoside Hydrolase Family 2

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:ETA_25680
OrganismiErwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)
Taxonomic identifieri465817 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeErwinia
Proteomesi
  • UP000001726 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003669871 – 1026Beta-galactosidaseAdd BLAST1026

Proteomic databases

PRIDEiB2VHN8

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi465817.ETA_25680

Structurei

3D structure databases

ProteinModelPortaliB2VHN8
SMRiB2VHN8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni539 – 542Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT Bacteria
COG3250 LUCA
HOGENOMiHOG000252443
KOiK01190
OMAiPSNWQLQ
OrthoDBiPOG091H07QB

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
2.70.98.10, 1 hit
HAMAPiMF_01687 Beta_gal, 1 hit
InterProiView protein in InterPro
IPR004199 B-gal_small/dom_5
IPR036156 Beta-gal/glucu_dom_sf
IPR011013 Gal_mutarotase_sf_dom
IPR008979 Galactose-bd-like_sf
IPR014718 GH-type_carb-bd
IPR006101 Glyco_hydro_2
IPR023232 Glyco_hydro_2_AS
IPR023933 Glyco_hydro_2_beta_Galsidase
IPR006103 Glyco_hydro_2_cat
IPR023230 Glyco_hydro_2_CS
IPR006102 Glyco_hydro_2_Ig-like
IPR006104 Glyco_hydro_2_N
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR032312 LacZ_4
PANTHERiPTHR10066:SF76 PTHR10066:SF76, 1 hit
PfamiView protein in Pfam
PF02929 Bgal_small_N, 1 hit
PF16353 DUF4981, 1 hit
PF00703 Glyco_hydro_2, 1 hit
PF02836 Glyco_hydro_2_C, 1 hit
PF02837 Glyco_hydro_2_N, 1 hit
PRINTSiPR00132 GLHYDRLASE2
SMARTiView protein in SMART
SM01038 Bgal_small_N, 1 hit
SUPFAMiSSF49303 SSF49303, 2 hits
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
SSF74650 SSF74650, 1 hit
PROSITEiView protein in PROSITE
PS00719 GLYCOSYL_HYDROL_F2_1, 1 hit
PS00608 GLYCOSYL_HYDROL_F2_2, 1 hit

Sequencei

Sequence statusi: Complete.

B2VHN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTDPLTLQ QLLAQRHWEN PALTQLNRLP AHTPLASWRN ADAARGDLAS
60 70 80 90 100
PSQRLLDGEW GFSYFDRPQA VPDAWINGDL PQVGRLSVPS NWQLSGYDAP
110 120 130 140 150
IYTNVQYPIP TDPPRVPDDN PTGCYSLTFH CQPAWLQSGQ TRIIFDGVNS
160 170 180 190 200
AFYLWCNGEF IGYSQDSRLP AEFDLSQRLL EGENRIAVMV LRWCDGSYLE
210 220 230 240 250
DQDMWRMSGI FRSVSLLHKP HVRLDDIHID TRLSPEYRSA QLRVLALCSL
260 270 280 290 300
TDASAYQLKV TLWRDDTLIA QHQQPFGTPV VDERGRYLDR TRLSLQIDQP
310 320 330 340 350
LLWNAETPHL YRAVIALLDA DGTLIEAEAC DVGFRQVEVS GGLLKLNGKA
360 370 380 390 400
LLIRGTNRHE HHPDRGQVMD EPAMIADILL MKQHNFNAVR CSHYPNHPLW
410 420 430 440 450
YRLCDRYGLY VVDEANIETH GMQPMNRLAD DPSWFSAFSE RVTRMVQRDR
460 470 480 490 500
NHACIIIWSL GNESGHGSTH DALYGWIKSD DPSRPVQYEG GGADTAASDI
510 520 530 540 550
ICPMYARVDR DQPFEAVPKW SIKKWIALPE ETRPLILCEY AHAMGNSLGG
560 570 580 590 600
FSRYWQAFRQ YPPLQGGFVW DWADQNLTRQ AADGSSWQAY GGDFGDMPND
610 620 630 640 650
RQFCMNGLVF ADRSPHPALF EAKRAQQFFQ FQLENTSPIT LGITSEYLFR
660 670 680 690 700
HSDNERLCWR IEHHGEQVAG GQITLNLPPE GTVSLMLGEL PSLAGELWLR
710 720 730 740 750
VEVIQPAATA WSPANHRVAW DGWQLPAALS LPKPDVPGEQ PRLHPQSQLI
760 770 780 790 800
EVVQAGQRWQ FCRQSGELIQ WLQADKPQLL SPLRDLFVRA PLDNDIGISE
810 820 830 840 850
ANRIDPHAWA ERWQRAGYYR LESQLLRLQT DILNDGVQIR SEQAWLADGE
860 870 880 890 900
PRFLSRKCYR INRQGEMLLE VEVDIAAGLP EPARIGLHCQ LAEVAEEVCW
910 920 930 940 950
LGLGPHENYP DRRLAAEFSR WQLPLGALST PYVFPCENGL RGGTRELTFG
960 970 980 990 1000
HWHIRGDFHF SLSRHSVEQL RKTSHRHLLR DEAGCWLTLD GFHMGVGGDD
1010 1020
SWSPSVDEEF LLRARQYRYR LILTRG
Length:1,026
Mass (Da):116,920
Last modified:July 1, 2008 - v1
Checksum:i63A6F9463A3FB870
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU468135 Genomic DNA Translation: CAO97614.1
RefSeqiWP_012442279.1, NC_010694.1

Genome annotation databases

EnsemblBacteriaiCAO97614; CAO97614; ETA_25680
KEGGieta:ETA_25680

Similar proteinsi

Entry informationi

Entry nameiBGAL_ERWT9
AccessioniPrimary (citable) accession number: B2VHN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: March 28, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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