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Protein

Beta-galactosidase

Gene

lacZ

Organism
Erwinia tasmaniensis (strain DSM 17950 / Et1/99)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041SubstrateUniRule annotation
Metal bindingi203 – 2031SodiumUniRule annotation
Binding sitei203 – 2031SubstrateUniRule annotation
Sitei359 – 3591Transition state stabilizerUniRule annotation
Sitei393 – 3931Transition state stabilizerUniRule annotation
Metal bindingi418 – 4181Magnesium 1UniRule annotation
Metal bindingi420 – 4201Magnesium 1UniRule annotation
Active sitei463 – 4631Proton donorUniRule annotation
Metal bindingi463 – 4631Magnesium 1UniRule annotation
Binding sitei463 – 4631SubstrateUniRule annotation
Active sitei539 – 5391NucleophileUniRule annotation
Metal bindingi599 – 5991Magnesium 2UniRule annotation
Metal bindingi603 – 6031Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi606 – 6061SodiumUniRule annotation
Binding sitei606 – 6061SubstrateUniRule annotation
Binding sitei1002 – 10021SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciETAS465817:GI36-2651-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:ETA_25680
OrganismiErwinia tasmaniensis (strain DSM 17950 / Et1/99)
Taxonomic identifieri338565 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia
ProteomesiUP000001726: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10261026Beta-galactosidasePRO_0000366987Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi465817.ETA_25680.

Structurei

3D structure databases

ProteinModelPortaliB2VHN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni539 – 5424Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiTNRHEHH.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2VHN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTDPLTLQ QLLAQRHWEN PALTQLNRLP AHTPLASWRN ADAARGDLAS
60 70 80 90 100
PSQRLLDGEW GFSYFDRPQA VPDAWINGDL PQVGRLSVPS NWQLSGYDAP
110 120 130 140 150
IYTNVQYPIP TDPPRVPDDN PTGCYSLTFH CQPAWLQSGQ TRIIFDGVNS
160 170 180 190 200
AFYLWCNGEF IGYSQDSRLP AEFDLSQRLL EGENRIAVMV LRWCDGSYLE
210 220 230 240 250
DQDMWRMSGI FRSVSLLHKP HVRLDDIHID TRLSPEYRSA QLRVLALCSL
260 270 280 290 300
TDASAYQLKV TLWRDDTLIA QHQQPFGTPV VDERGRYLDR TRLSLQIDQP
310 320 330 340 350
LLWNAETPHL YRAVIALLDA DGTLIEAEAC DVGFRQVEVS GGLLKLNGKA
360 370 380 390 400
LLIRGTNRHE HHPDRGQVMD EPAMIADILL MKQHNFNAVR CSHYPNHPLW
410 420 430 440 450
YRLCDRYGLY VVDEANIETH GMQPMNRLAD DPSWFSAFSE RVTRMVQRDR
460 470 480 490 500
NHACIIIWSL GNESGHGSTH DALYGWIKSD DPSRPVQYEG GGADTAASDI
510 520 530 540 550
ICPMYARVDR DQPFEAVPKW SIKKWIALPE ETRPLILCEY AHAMGNSLGG
560 570 580 590 600
FSRYWQAFRQ YPPLQGGFVW DWADQNLTRQ AADGSSWQAY GGDFGDMPND
610 620 630 640 650
RQFCMNGLVF ADRSPHPALF EAKRAQQFFQ FQLENTSPIT LGITSEYLFR
660 670 680 690 700
HSDNERLCWR IEHHGEQVAG GQITLNLPPE GTVSLMLGEL PSLAGELWLR
710 720 730 740 750
VEVIQPAATA WSPANHRVAW DGWQLPAALS LPKPDVPGEQ PRLHPQSQLI
760 770 780 790 800
EVVQAGQRWQ FCRQSGELIQ WLQADKPQLL SPLRDLFVRA PLDNDIGISE
810 820 830 840 850
ANRIDPHAWA ERWQRAGYYR LESQLLRLQT DILNDGVQIR SEQAWLADGE
860 870 880 890 900
PRFLSRKCYR INRQGEMLLE VEVDIAAGLP EPARIGLHCQ LAEVAEEVCW
910 920 930 940 950
LGLGPHENYP DRRLAAEFSR WQLPLGALST PYVFPCENGL RGGTRELTFG
960 970 980 990 1000
HWHIRGDFHF SLSRHSVEQL RKTSHRHLLR DEAGCWLTLD GFHMGVGGDD
1010 1020
SWSPSVDEEF LLRARQYRYR LILTRG
Length:1,026
Mass (Da):116,920
Last modified:July 1, 2008 - v1
Checksum:i63A6F9463A3FB870
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU468135 Genomic DNA. Translation: CAO97614.1.
RefSeqiYP_001908490.1. NC_010694.1.

Genome annotation databases

EnsemblBacteriaiCAO97614; CAO97614; ETA_25680.
GeneIDi6299686.
KEGGieta:ETA_25680.
PATRICi20431029. VBIErwTas9546_2664.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU468135 Genomic DNA. Translation: CAO97614.1.
RefSeqiYP_001908490.1. NC_010694.1.

3D structure databases

ProteinModelPortaliB2VHN8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi465817.ETA_25680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAO97614; CAO97614; ETA_25680.
GeneIDi6299686.
KEGGieta:ETA_25680.
PATRICi20431029. VBIErwTas9546_2664.

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiTNRHEHH.
OrthoDBiEOG6XWV0T.

Enzyme and pathway databases

BioCyciETAS465817:GI36-2651-MONOMER.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia."
    Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K.
    Environ. Microbiol. 10:2211-2222(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17950 / Et1/99.

Entry informationi

Entry nameiBGAL_ERWT9
AccessioniPrimary (citable) accession number: B2VHN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: January 7, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.