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B2VHN8

- BGAL_ERWT9

UniProt

B2VHN8 - BGAL_ERWT9

Protein

Beta-galactosidase

Gene

lacZ

Organism
Erwinia tasmaniensis (strain DSM 17950 / Et1/99)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041SubstrateUniRule annotation
    Metal bindingi203 – 2031SodiumUniRule annotation
    Binding sitei203 – 2031SubstrateUniRule annotation
    Sitei359 – 3591Transition state stabilizerUniRule annotation
    Sitei393 – 3931Transition state stabilizerUniRule annotation
    Metal bindingi418 – 4181Magnesium 1UniRule annotation
    Metal bindingi420 – 4201Magnesium 1UniRule annotation
    Active sitei463 – 4631Proton donorUniRule annotation
    Metal bindingi463 – 4631Magnesium 1UniRule annotation
    Binding sitei463 – 4631SubstrateUniRule annotation
    Active sitei539 – 5391NucleophileUniRule annotation
    Metal bindingi599 – 5991Magnesium 2UniRule annotation
    Metal bindingi603 – 6031Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi606 – 6061SodiumUniRule annotation
    Binding sitei606 – 6061SubstrateUniRule annotation
    Binding sitei1002 – 10021SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciETAS465817:GI36-2651-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:ETA_25680
    OrganismiErwinia tasmaniensis (strain DSM 17950 / Et1/99)
    Taxonomic identifieri338565 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia
    ProteomesiUP000001726: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10261026Beta-galactosidasePRO_0000366987Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi465817.ETA_25680.

    Structurei

    3D structure databases

    ProteinModelPortaliB2VHN8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni539 – 5424Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiNPPFVPK.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2VHN8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKTDPLTLQ QLLAQRHWEN PALTQLNRLP AHTPLASWRN ADAARGDLAS     50
    PSQRLLDGEW GFSYFDRPQA VPDAWINGDL PQVGRLSVPS NWQLSGYDAP 100
    IYTNVQYPIP TDPPRVPDDN PTGCYSLTFH CQPAWLQSGQ TRIIFDGVNS 150
    AFYLWCNGEF IGYSQDSRLP AEFDLSQRLL EGENRIAVMV LRWCDGSYLE 200
    DQDMWRMSGI FRSVSLLHKP HVRLDDIHID TRLSPEYRSA QLRVLALCSL 250
    TDASAYQLKV TLWRDDTLIA QHQQPFGTPV VDERGRYLDR TRLSLQIDQP 300
    LLWNAETPHL YRAVIALLDA DGTLIEAEAC DVGFRQVEVS GGLLKLNGKA 350
    LLIRGTNRHE HHPDRGQVMD EPAMIADILL MKQHNFNAVR CSHYPNHPLW 400
    YRLCDRYGLY VVDEANIETH GMQPMNRLAD DPSWFSAFSE RVTRMVQRDR 450
    NHACIIIWSL GNESGHGSTH DALYGWIKSD DPSRPVQYEG GGADTAASDI 500
    ICPMYARVDR DQPFEAVPKW SIKKWIALPE ETRPLILCEY AHAMGNSLGG 550
    FSRYWQAFRQ YPPLQGGFVW DWADQNLTRQ AADGSSWQAY GGDFGDMPND 600
    RQFCMNGLVF ADRSPHPALF EAKRAQQFFQ FQLENTSPIT LGITSEYLFR 650
    HSDNERLCWR IEHHGEQVAG GQITLNLPPE GTVSLMLGEL PSLAGELWLR 700
    VEVIQPAATA WSPANHRVAW DGWQLPAALS LPKPDVPGEQ PRLHPQSQLI 750
    EVVQAGQRWQ FCRQSGELIQ WLQADKPQLL SPLRDLFVRA PLDNDIGISE 800
    ANRIDPHAWA ERWQRAGYYR LESQLLRLQT DILNDGVQIR SEQAWLADGE 850
    PRFLSRKCYR INRQGEMLLE VEVDIAAGLP EPARIGLHCQ LAEVAEEVCW 900
    LGLGPHENYP DRRLAAEFSR WQLPLGALST PYVFPCENGL RGGTRELTFG 950
    HWHIRGDFHF SLSRHSVEQL RKTSHRHLLR DEAGCWLTLD GFHMGVGGDD 1000
    SWSPSVDEEF LLRARQYRYR LILTRG 1026
    Length:1,026
    Mass (Da):116,920
    Last modified:July 1, 2008 - v1
    Checksum:i63A6F9463A3FB870
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU468135 Genomic DNA. Translation: CAO97614.1.
    RefSeqiYP_001908490.1. NC_010694.1.

    Genome annotation databases

    EnsemblBacteriaiCAO97614; CAO97614; ETA_25680.
    GeneIDi6299686.
    KEGGieta:ETA_25680.
    PATRICi20431029. VBIErwTas9546_2664.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU468135 Genomic DNA. Translation: CAO97614.1 .
    RefSeqi YP_001908490.1. NC_010694.1.

    3D structure databases

    ProteinModelPortali B2VHN8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 465817.ETA_25680.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAO97614 ; CAO97614 ; ETA_25680 .
    GeneIDi 6299686.
    KEGGi eta:ETA_25680.
    PATRICi 20431029. VBIErwTas9546_2664.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi NPPFVPK.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci ETAS465817:GI36-2651-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia."
      Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K.
      Environ. Microbiol. 10:2211-2222(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17950 / Et1/99.

    Entry informationi

    Entry nameiBGAL_ERWT9
    AccessioniPrimary (citable) accession number: B2VHN8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3