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B2VHN8

- BGAL_ERWT9

UniProt

B2VHN8 - BGAL_ERWT9

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Protein
Beta-galactosidase
Gene
lacZ, ETA_25680
Organism
Erwinia tasmaniensis (strain DSM 17950 / Et1/99)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041Substrate By similarity
Metal bindingi203 – 2031Sodium By similarity
Binding sitei203 – 2031Substrate By similarity
Sitei359 – 3591Transition state stabilizer By similarity
Sitei393 – 3931Transition state stabilizer By similarity
Metal bindingi418 – 4181Magnesium 1 By similarity
Metal bindingi420 – 4201Magnesium 1 By similarity
Active sitei463 – 4631Proton donor By similarity
Metal bindingi463 – 4631Magnesium 1 By similarity
Binding sitei463 – 4631Substrate By similarity
Active sitei539 – 5391Nucleophile By similarity
Metal bindingi599 – 5991Magnesium 2 By similarity
Metal bindingi603 – 6031Sodium; via carbonyl oxygen By similarity
Metal bindingi606 – 6061Sodium By similarity
Binding sitei606 – 6061Substrate By similarity
Binding sitei1002 – 10021Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciETAS465817:GI36-2651-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:ETA_25680
OrganismiErwinia tasmaniensis (strain DSM 17950 / Et1/99)
Taxonomic identifieri338565 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia
ProteomesiUP000001726: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10261026Beta-galactosidaseUniRule annotation
PRO_0000366987Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi465817.ETA_25680.

Structurei

3D structure databases

ProteinModelPortaliB2VHN8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni539 – 5424Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiNPPFVPK.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2VHN8-1 [UniParc]FASTAAdd to Basket

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MAKTDPLTLQ QLLAQRHWEN PALTQLNRLP AHTPLASWRN ADAARGDLAS     50
PSQRLLDGEW GFSYFDRPQA VPDAWINGDL PQVGRLSVPS NWQLSGYDAP 100
IYTNVQYPIP TDPPRVPDDN PTGCYSLTFH CQPAWLQSGQ TRIIFDGVNS 150
AFYLWCNGEF IGYSQDSRLP AEFDLSQRLL EGENRIAVMV LRWCDGSYLE 200
DQDMWRMSGI FRSVSLLHKP HVRLDDIHID TRLSPEYRSA QLRVLALCSL 250
TDASAYQLKV TLWRDDTLIA QHQQPFGTPV VDERGRYLDR TRLSLQIDQP 300
LLWNAETPHL YRAVIALLDA DGTLIEAEAC DVGFRQVEVS GGLLKLNGKA 350
LLIRGTNRHE HHPDRGQVMD EPAMIADILL MKQHNFNAVR CSHYPNHPLW 400
YRLCDRYGLY VVDEANIETH GMQPMNRLAD DPSWFSAFSE RVTRMVQRDR 450
NHACIIIWSL GNESGHGSTH DALYGWIKSD DPSRPVQYEG GGADTAASDI 500
ICPMYARVDR DQPFEAVPKW SIKKWIALPE ETRPLILCEY AHAMGNSLGG 550
FSRYWQAFRQ YPPLQGGFVW DWADQNLTRQ AADGSSWQAY GGDFGDMPND 600
RQFCMNGLVF ADRSPHPALF EAKRAQQFFQ FQLENTSPIT LGITSEYLFR 650
HSDNERLCWR IEHHGEQVAG GQITLNLPPE GTVSLMLGEL PSLAGELWLR 700
VEVIQPAATA WSPANHRVAW DGWQLPAALS LPKPDVPGEQ PRLHPQSQLI 750
EVVQAGQRWQ FCRQSGELIQ WLQADKPQLL SPLRDLFVRA PLDNDIGISE 800
ANRIDPHAWA ERWQRAGYYR LESQLLRLQT DILNDGVQIR SEQAWLADGE 850
PRFLSRKCYR INRQGEMLLE VEVDIAAGLP EPARIGLHCQ LAEVAEEVCW 900
LGLGPHENYP DRRLAAEFSR WQLPLGALST PYVFPCENGL RGGTRELTFG 950
HWHIRGDFHF SLSRHSVEQL RKTSHRHLLR DEAGCWLTLD GFHMGVGGDD 1000
SWSPSVDEEF LLRARQYRYR LILTRG 1026
Length:1,026
Mass (Da):116,920
Last modified:July 1, 2008 - v1
Checksum:i63A6F9463A3FB870
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU468135 Genomic DNA. Translation: CAO97614.1.
RefSeqiYP_001908490.1. NC_010694.1.

Genome annotation databases

EnsemblBacteriaiCAO97614; CAO97614; ETA_25680.
GeneIDi6299686.
KEGGieta:ETA_25680.
PATRICi20431029. VBIErwTas9546_2664.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU468135 Genomic DNA. Translation: CAO97614.1 .
RefSeqi YP_001908490.1. NC_010694.1.

3D structure databases

ProteinModelPortali B2VHN8.
ModBasei Search...

Protein-protein interaction databases

STRINGi 465817.ETA_25680.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAO97614 ; CAO97614 ; ETA_25680 .
GeneIDi 6299686.
KEGGi eta:ETA_25680.
PATRICi 20431029. VBIErwTas9546_2664.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi NPPFVPK.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci ETAS465817:GI36-2651-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia."
    Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K.
    Environ. Microbiol. 10:2211-2222(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17950 / Et1/99.

Entry informationi

Entry nameiBGAL_ERWT9
AccessioniPrimary (citable) accession number: B2VHN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi