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B2VGR6

- SYI_ERWT9

UniProt

B2VGR6 - SYI_ERWT9

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Protein
Isoleucine--tRNA ligase
Gene
ileS, ETA_07120
Organism
Erwinia tasmaniensis (strain DSM 17950 / Et1/99)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylate By similarity
Binding sitei605 – 6051ATP By similarity
Metal bindingi901 – 9011Zinc By similarity
Metal bindingi904 – 9041Zinc By similarity
Metal bindingi921 – 9211Zinc By similarity
Metal bindingi924 – 9241Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciETAS465817:GI36-751-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:ETA_07120
OrganismiErwinia tasmaniensis (strain DSM 17950 / Et1/99)
Taxonomic identifieri338565 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia
ProteomesiUP000001726: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189164Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi465817.ETA_07120.

Structurei

3D structure databases

ProteinModelPortaliB2VGR6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi602 – 6065"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2VGR6-1 [UniParc]FASTAAdd to Basket

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MSDYKSTLNL PETGFPMRGD LAKREPGMLQ RWYDDKLYSI IREAKKGKKT    50
FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKGMAGYDSP YVPGWDCHGL 100
PIEHKVEQTI GKPGEKVSAA EFRAACRQYA AEQVEGQKAD FIRLGVLGDW 150
DRPYLTMDFK TEANIIRALG KIIGNGHLHK GAKPVHWCLD CRSALAEAEV 200
EYYDKTSPSI DVMFDAVDKD AVQAKFGAAH VNGPISLVIW TTTPWTMPAN 250
RAISLHPEFD YQLVQVEGRA LILAKDMVDS VMKRVGVTQW TVLGDVQGAA 300
LELMGFQHPF LAHVSPVVLG EHVTLEAGTG AVHTAPGHGP DDYVIGQKYG 350
IETANPVGPD GSFLPGTYPT LDGLNVFKAN DTIVELLREK GALLHLEKLH 400
HSYPHCWRHK TPIIFRATPQ WFISMDQKGL RAQSLKEIKG VQWIPDWGQA 450
RIESMVANRP DWCISRQRTW GVPMALFVHK DTEQLHPDSL ELMEKVALRV 500
EQDGIQAWWD LDARELMGAD ADNYVKVPDT LDVWFDSGST SYSVVDARPE 550
FGGSAPDLYL EGSDQHRGWF MSSLMISTAM KGKAPYRQVL THGFTVDGQG 600
RKMSKSLGNT VSPQDVMNKL GADILRLWVA STDYSGEIAV SDEILKRSAD 650
SYRRIRNTAR FLLANLAGFN PETDKVKPEE MVVVDRWAVG RALAAQNDIV 700
ASYEAYDFHE VVQRLMQFCS VEMGSFYLDI IKDRQYTAKA DGLARRSCQT 750
ALWYIVEALV RWMAPIMSFT ADEIWGYLPG KRAQYVFTEE WFDGLFSLED 800
NQPMNDAYWA ELLKVRGEVN KVIEQARADK RVGGSLEASV TLYADAQLAE 850
KLTSLGEELR FVLLTSGAEV ADYAGAPDDA QQSETVKGLK IALRKAEGEK 900
CPRCWHYTSD IGQNAEHADM CGRCVTNVAG SGEERKFA 938
Length:938
Mass (Da):104,632
Last modified:July 1, 2008 - v1
Checksum:i315F13E005C85109
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU468135 Genomic DNA. Translation: CAO95758.1.
RefSeqiYP_001906656.1. NC_010694.1.

Genome annotation databases

EnsemblBacteriaiCAO95758; CAO95758; ETA_07120.
GeneIDi6299788.
KEGGieta:ETA_07120.
PATRICi20427144. VBIErwTas9546_0763.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU468135 Genomic DNA. Translation: CAO95758.1 .
RefSeqi YP_001906656.1. NC_010694.1.

3D structure databases

ProteinModelPortali B2VGR6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 465817.ETA_07120.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAO95758 ; CAO95758 ; ETA_07120 .
GeneIDi 6299788.
KEGGi eta:ETA_07120.
PATRICi 20427144. VBIErwTas9546_0763.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci ETAS465817:GI36-751-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia."
    Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K.
    Environ. Microbiol. 10:2211-2222(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17950 / Et1/99.

Entry informationi

Entry nameiSYI_ERWT9
AccessioniPrimary (citable) accession number: B2VGR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi