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B2VGR6

- SYI_ERWT9

UniProt

B2VGR6 - SYI_ERWT9

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Erwinia tasmaniensis (strain DSM 17950 / Et1/99)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
    Binding sitei605 – 6051ATPUniRule annotation
    Metal bindingi901 – 9011ZincUniRule annotation
    Metal bindingi904 – 9041ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciETAS465817:GI36-751-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:ETA_07120
    OrganismiErwinia tasmaniensis (strain DSM 17950 / Et1/99)
    Taxonomic identifieri338565 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia
    ProteomesiUP000001726: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 938938Isoleucine--tRNA ligasePRO_1000189164Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi465817.ETA_07120.

    Structurei

    3D structure databases

    ProteinModelPortaliB2VGR6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi602 – 6065"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2VGR6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKSTLNL PETGFPMRGD LAKREPGMLQ RWYDDKLYSI IREAKKGKKT    50
    FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKGMAGYDSP YVPGWDCHGL 100
    PIEHKVEQTI GKPGEKVSAA EFRAACRQYA AEQVEGQKAD FIRLGVLGDW 150
    DRPYLTMDFK TEANIIRALG KIIGNGHLHK GAKPVHWCLD CRSALAEAEV 200
    EYYDKTSPSI DVMFDAVDKD AVQAKFGAAH VNGPISLVIW TTTPWTMPAN 250
    RAISLHPEFD YQLVQVEGRA LILAKDMVDS VMKRVGVTQW TVLGDVQGAA 300
    LELMGFQHPF LAHVSPVVLG EHVTLEAGTG AVHTAPGHGP DDYVIGQKYG 350
    IETANPVGPD GSFLPGTYPT LDGLNVFKAN DTIVELLREK GALLHLEKLH 400
    HSYPHCWRHK TPIIFRATPQ WFISMDQKGL RAQSLKEIKG VQWIPDWGQA 450
    RIESMVANRP DWCISRQRTW GVPMALFVHK DTEQLHPDSL ELMEKVALRV 500
    EQDGIQAWWD LDARELMGAD ADNYVKVPDT LDVWFDSGST SYSVVDARPE 550
    FGGSAPDLYL EGSDQHRGWF MSSLMISTAM KGKAPYRQVL THGFTVDGQG 600
    RKMSKSLGNT VSPQDVMNKL GADILRLWVA STDYSGEIAV SDEILKRSAD 650
    SYRRIRNTAR FLLANLAGFN PETDKVKPEE MVVVDRWAVG RALAAQNDIV 700
    ASYEAYDFHE VVQRLMQFCS VEMGSFYLDI IKDRQYTAKA DGLARRSCQT 750
    ALWYIVEALV RWMAPIMSFT ADEIWGYLPG KRAQYVFTEE WFDGLFSLED 800
    NQPMNDAYWA ELLKVRGEVN KVIEQARADK RVGGSLEASV TLYADAQLAE 850
    KLTSLGEELR FVLLTSGAEV ADYAGAPDDA QQSETVKGLK IALRKAEGEK 900
    CPRCWHYTSD IGQNAEHADM CGRCVTNVAG SGEERKFA 938
    Length:938
    Mass (Da):104,632
    Last modified:July 1, 2008 - v1
    Checksum:i315F13E005C85109
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU468135 Genomic DNA. Translation: CAO95758.1.
    RefSeqiYP_001906656.1. NC_010694.1.

    Genome annotation databases

    EnsemblBacteriaiCAO95758; CAO95758; ETA_07120.
    GeneIDi6299788.
    KEGGieta:ETA_07120.
    PATRICi20427144. VBIErwTas9546_0763.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU468135 Genomic DNA. Translation: CAO95758.1 .
    RefSeqi YP_001906656.1. NC_010694.1.

    3D structure databases

    ProteinModelPortali B2VGR6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 465817.ETA_07120.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAO95758 ; CAO95758 ; ETA_07120 .
    GeneIDi 6299788.
    KEGGi eta:ETA_07120.
    PATRICi 20427144. VBIErwTas9546_0763.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci ETAS465817:GI36-751-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia."
      Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K.
      Environ. Microbiol. 10:2211-2222(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17950 / Et1/99.

    Entry informationi

    Entry nameiSYI_ERWT9
    AccessioniPrimary (citable) accession number: B2VGR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3