B2VG69 (ILVC_ERWT9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	ETA_01900

Organism

Erwinia tasmaniensis (strain DSM 17950 / Et1/99) [Complete proteome] [HAMAP]

Taxonomic identifier

338565 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Erwinia

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000190952
Sites
	Active site	132	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	B2VG69 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 801DCFE1A6497E5F	FASTA	492	53,904
	10 20 30 40 50 60 MANYFNTLNL RNQLAQLGKC RFMAREEFAD EASYLKGKKV VIVGCGAQGL NQGLNMRDSG 70 80 90 100 110 120 LDIAYALRAE AIAEKRASWR KATENGFKVG TYEELIPQAD LVVNLTPDKQ HTSVVQAVQP 130 140 150 160 170 180 LMKDGAALGY SHGFNIVEVG EQVRKDVTVV MVAPKCPGTE VREEYKRGFG VPTLIAVHPE 190 200 210 220 230 240 NDPKGEGMAI AKAWAAATGG HRAGVLESSF VAEVKSDLMG EQTILCGMLQ AGSLLCFDKL 250 260 270 280 290 300 VAEGTDAAYA EKLIQFGWET ITEALKQGGI TLMMDRLSNP AKVRAYALSE QLKTIMAPLF 310 320 330 340 350 360 QKHMDDIISG EFSSGMMADW ANDDKKLLGW REETGKTAFE TAAQFEGKIG EQDYFDQGVV 370 380 390 400 410 420 MIAMVKAGVE LAFETMVAAG IVEESAYYES LHELPLIANT IARKRLYEMN VVISDTAEYG 430 440 450 460 470 480 NYLFSYAAVP LLQEFMTTLQ AGDLGKQAQT STSVDNAQLR DVNEAIRHHD IETVGRKLRG 490 YMTDMKRIAV AG

References

[1]

"The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia." Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K. Environ. Microbiol. 10:2211-2222(2008) [PubMed: 18462403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17950 / Et1/99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU468135 Genomic DNA. Translation: CAO95236.1.
RefSeq	YP_001906144.1. NC_010694.1.
3D structure databases
ProteinModelPortal	B2VG69.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6299192.
GenomeReviews	Gene locus ETA_01900 in contig CU468135_GR.
PATRIC	20426054. VBIErwTas9546_0237.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG297205.
OMA	EKHMDDI.
ProtClustDB	PRK05225.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit. PTHR21371:SF2. PTHR21371:SF2. 1 hit.
Pfam	PF01450. IlvC. 2 hits. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
SUPFAM	SSF48179. 6DGDH_C_like. 2 hits.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_ERWT9

Accession

Primary (citable) accession number: B2VG69

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program