B2VEK0 (NADE_ERWT9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	ETA_18510

Organism

Erwinia tasmaniensis (strain DSM 17950 / Et1/99) [Complete proteome] [HAMAP]

Taxonomic identifier

338565 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Erwinia

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099021
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2VEK0 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: E4722CA049BDF464	FASTA	275	30,536
	10 20 30 40 50 60 MALQQDIIEA LGVKPTIDAD EEIRISVDFL KSYLKRYPFL KSLVLGISGG QDSTLTGKLC 70 80 90 100 110 120 QMAMTELRAE SGDSDYQFIA VRLPHGVQAD EQDCQDAITF IQPDRVLTVN IKAAVQASEQ 130 140 150 160 170 180 ALREAGITLS DFIRGNEKAR ERMKVQYSIA GMNAGVVVGT DHAAEAVTGF FTKYGDGGTD 190 200 210 220 230 240 INPIFRLNKG QGKRLLNALG CPEHLWLKHP TADLEDDRPG LQDEVALGVT YEMIDRYLQG 250 260 270 ESIDPAAAKI IEGWYVKTEH KRRTPITVFD DFWKK

References

[1]

"The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia." Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K. Environ. Microbiol. 10:2211-2222(2008) [PubMed: 18462403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17950 / Et1/99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU468135 Genomic DNA. Translation: CAO96897.1.
RefSeq	YP_001907784.1. NC_010694.1.
3D structure databases
ProteinModelPortal	B2VEK0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6300782.
GenomeReviews	Gene locus ETA_18510 in contig CU468135_GR.
PATRIC	20429533. VBIErwTas9546_1935.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	GLNKRRV.
ProtClustDB	PRK00768.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_ERWT9

Accession

Primary (citable) accession number: B2VEK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program