B2VDD5 (RLMG_ERWT9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	ETA_28960

Organism

Erwinia tasmaniensis (strain DSM 17950 / Et1/99) [Complete proteome] [HAMAP]

Taxonomic identifier

338565 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Erwinia

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366454

Sequences

	Sequence		Length	Mass (Da)
	B2VDD5 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 77F21740682FC2A5	FASTA	375	41,976
	10 20 30 40 50 60 MSQLELSNRM LTLHRFPQMR EESPLQAWDA ADEYLLNHLD NLPVNGPTLI FNDTFGALAC 70 80 90 100 110 120 ALAGEGVWSI SDSWLNQQAT RQNLALNQLD EGDVRLLDSL APLPGAPARV LIKVPKTLAL 130 140 150 160 170 180 LEKQLRALRA VVTPETQIVA AGKAKEIHTS TLQLFEKILG PTTTSLAWKK ARLIYATFTQ 190 200 210 220 230 240 PELAESEVIS RWPLDGTPWQ IHNHANVFAR GGLDIGARFF MQHLPDDIDG EIVDLGCGNG 250 260 270 280 290 300 VIGLMALRQN PLAQVHFLDE SYMAVASSRM NVELNCPDDL ARCEFRVNNA LAGYPSDRLH 310 320 330 340 350 360 AVLCNPPFHQ QNAVTDHIAW QMFRDARRCL QYGGELRIVG NRHLDYYHKM KKLFGNCTTV 370 ATHQKFVILR SVKMP

References

[1]

"The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia." Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K. Environ. Microbiol. 10:2211-2222(2008) [PubMed: 18462403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17950 / Et1/99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU468135 Genomic DNA. Translation: CAO97942.1.
RefSeq	YP_001908811.1. NC_010694.1.
3D structure databases
ProteinModelPortal	B2VDD5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6298845.
GenomeReviews	Gene locus ETA_28960 in contig CU468135_GR.
PATRIC	20431709. VBIErwTas9546_2989.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG321931.
OMA	NRHLGYH.
ProtClustDB	CLSK2543780.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_ERWT9

Accession

Primary (citable) accession number: B2VDD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program