B2VCD1 (GHRB_ERWT9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyoxylate/hydroxypyruvate reductase B
EC=1.1.1.79
EC=1.1.1.81

Gene names

Name:	ghrB
Ordered Locus Names:	ETA_34410

Organism

Erwinia tasmaniensis (strain DSM 17950 / Et1/99) [Complete proteome] [HAMAP]

Taxonomic identifier

338565 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Erwinia

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP-Rule MF_01667
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP-Rule MF_01667 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP-Rule MF_01667
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01667
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01667.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Glyoxylate/hydroxypyruvate reductase B HAMAP-Rule MF_01667		PRO_1000187291
Sites
	Active site	237	1	By similarity
	Active site	266	1	By similarity
	Active site	285	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2VCD1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 9BE94B1F2E0B6FB4	FASTA	321	35,117
	10 20 30 40 50 60 MKPSVVLYKS LPEDLRARLD EHCHVTEING LTAENIAKHE DVFRQAEGIL GSGGKVDAEF 70 80 90 100 110 120 LRQAPKLRVA SSISVGYDNF DVAALNDRGV LLMHTPTVLT ETVADTMMAL VLSSARRVVE 130 140 150 160 170 180 MAERVKSGEW RGSISSDWFG IDVHHKKLGI LGMGRIGLAL AQRAHLGFGM PILYNARKHH 190 200 210 220 230 240 DEAEQRFDAE YCDLDTLLAE SDFLCISLPL TEQTHHLIGR EQLAKMKRSA ILINAGRGPV 250 260 270 280 290 300 VDEQALIAAL KDGTLHAAGL DVFEQEPLPV SSELLALRNV VALPHIGSAT HETRYGMAKD 310 320 AVDNLIAALN GKVEKNCVNP R

References

[1]

"The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia." Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K. Environ. Microbiol. 10:2211-2222(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17950 / Et1/99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU468135 Genomic DNA. Translation: CAO98487.1.
RefSeq	YP_001909347.1. NC_010694.1.
3D structure databases
ProteinModelPortal	B2VCD1.
ModBase	Search...
Protein-protein interaction databases
STRING	465817.ETA_34410.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAO98487; CAO98487; ETA_34410.
GeneID	6298409.
KEGG	eta:ETA_34410.
PATRIC	20432811. VBIErwTas9546_3532.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1052.
HOGENOM	HOG000136700.
KO	K00090.
OMA	ERSMKPS.
ProtClustDB	PRK15409.
Enzyme and pathway databases
BioCyc	ETAS465817:GI36-3549-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
HAMAP	MF_01667. 2-Hacid_dh_C_GhrB.
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR023756. Glyo/OHPyrv_Rdtase_B. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRB_ERWT9

Accession

Primary (citable) accession number: B2VCD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	July 1, 2008
Last modified:	May 29, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program