B2VBT9BIOB_ERWT9Biotin synthase2.8.1.6bioBETA_22660Erwinia tasmaniensis (strain DSM 17950 / CFBP 7177 / CIP 109463 / NCPPB 4357 / Et1/99)BacteriaPseudomonadotaGammaproteobacteriaEnterobacteralesErwiniaceaeErwiniaThe genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia.NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.(4R,5S)-dethiobiotin + [sulfur carrier]-SH + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine + [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin][4Fe-4S] clusterBinds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.[2Fe-2S] clusterBinds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.Homodimer.Belongs to the radical SAM superfamily. Biotin synthase family.2Fe-2S4Fe-4SBiotin biosynthesisIronIron-sulfurMetal-bindingReference proteomeS-adenosyl-L-methionineTransferase[4Fe-4S] cluster4Fe-4S-S-AdoMet[4Fe-4S] cluster4Fe-4S-S-AdoMet[4Fe-4S] cluster4Fe-4S-S-AdoMet[2Fe-2S] cluster[2Fe-2S] cluster[2Fe-2S] cluster[2Fe-2S] clusterMATRWTLSQAQALFETPFLELMFEAQQIHRHYFDPRQVQVSTLLSIKTGACPEDCKYCPQSARYKTGLEAERLMEVEEVLSSARKAKAAGSGRFCMGAAWKNPHERDMPYLEKMVQGVKEMGMETCMTLGSLSEQQAQRLAHAGLDFYNHNLDTSPEFYGSIITTRSYQERLDTLGKVREAGIKVCSGGIVGLGETVQDRAGLLVQLANLPEPPESVPINMLVKVKGTPMADNDDVDPFDFVRTIAVARIMMPRSHVRLSAGREQMSEQTQAMCFMAGANSIFYGCKLLTTPNPDEDKDLLLFRKLGLNPRHGTTESGDNAQQQALAAQLLSADTEQFYNAAR
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