Reviewed, UniProtKB/Swiss-Prot B2VBN3 (SYQ_ERWT9)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	ETA_23290

Organism

Erwinia tasmaniensis (strain DSM 17950 / Et1/99) [Complete proteome] [HAMAP]

Taxonomic identifier

338565 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Erwinia

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_1000095491
Regions
	Motif	34 – 44	11	"HIGH" region HAMAP MF_00126
	Motif	268 – 272	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2VBN3-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: CBDDCF5EF7089B7B	FASTA	555	63,791
	10 20 30 40 50 60 MSEAEARPTN FIRQIIDEDL ENGKHSMVHT RFPPEPNGYL HIGHAKSICL NFGIAQDYKG 70 80 90 100 110 120 QCNLRFDDTN PVKEDLEFVE SIKRDVQWLG FQWSGEVRYS SDYFEQLHNY AVELIGKGLA 130 140 150 160 170 180 YVDELSPEQI REYRGSLTSA GKNSPYRDRS VAENLELFAK MRNGEFAEGT ACLRAKIDMA 190 200 210 220 230 240 SNFIVLRDPV IYRIKFADHH QTGNRWCIYP MYDFTHCISD ALEGITHSLC TLEFQDNRRL 250 260 270 280 290 300 YDWVLDNITI PVHPRQYEFS RLNLEYAIMS KRKLNLLVTE KVVEGWDDPR MLTVSGLRRR 310 320 330 340 350 360 GYSAGSIREF CRRIGVTKQD NIVEMAALES CIRDDLNENA PRAMAVLDAV KVVIENLPAH 370 380 390 400 410 420 HEQTICMPNH PNRPEMGTRQ VPFSREIYID RADFREEANK HYKRLVLGKE VRLRNAYVIK 430 440 450 460 470 480 AERIAKDEEG NITCLFCSCD IDTLSKDPAD GRKVKGVIHW VSAEHALLAE FRLYDRLFSV 490 500 510 520 530 540 PNPAAAEDFL TTINPESLVI RQGFVEPGMQ QALASAPYQF EREGYFCADS VYSTSNKLVF 550 NRTVGLRDTW AKNGE

References

[1]

"The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia." Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K. Environ. Microbiol. 10:2211-2222(2008) [PubMed: 18462403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU468135 Genomic DNA. Translation: CAO97375.1.
RefSeq	YP_001908253.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6297923.
GenomeReviews	Gene locus ETA_23290 in contig CU468135_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RMPTIAG.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_ERWT9

Accession

Primary (citable) accession number: B2VBN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)