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B2VBM4 (SYL_ERWT9) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:ETA_23470
OrganismErwinia tasmaniensis (strain DSM 17950 / Et1/99) [Complete proteome] [HAMAP]
Taxonomic identifier338565 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia

Protein attributes

Sequence length860 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 860860Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091317

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2VBM4 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: EE1F9F215ED7E12F

FASTA86096,742
        10         20         30         40         50         60 
MQEQYRPEEI ESNVQQHWDE KQTFKVTEDE GKEKYYCLSM LPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW TYANIDYMKN QLKLLGFGYD 

       130        140        150        160        170        180 
WNRELATCQP EYYRWEQWFF TKLYEKGLVY KKTSAVNWCP HDMTVLANEQ VIDGCCWRCD 

       190        200        210        220        230        240 
SKVERKEIPQ WFVKITDYAD ELLNDLDKLE SWPEQVKTMQ RNWIGRSEGV EIEFTVLNSE 

       250        260        270        280        290        300 
EKLSVYTTRP DTFMGVTYLA VAAGHPLAAQ AALNNPALAD FIAECRNTKV AEADMATMEK 

       310        320        330        340        350        360 
KGMATGLFAA HPLTGEKVPV WVANFVLMEY GTGAVMAVPG HDQRDWEFAS KYSLPIKPVI 

       370        380        390        400        410        420 
LAADGSEPDL SGSAMTEKGT LFNSGEFDGL SHEAGFDAIA AKLADKGVGE RKVNYRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMVTLEDG TVMPTPEDQL PVILPEDVVM DGITSPIKAD AEWAKTTVNG 

       490        500        510        520        530        540 
QPALRETDTF DTFMESSWYY ARYTCPNYDK GMLDPAAANY WLPVDQYVGG IEHAIMHLLY 

       550        560        570        580        590        600 
FRFFHKLLRD TGLVNSDEPA KRLLCQGMVL ADAFYFTGSN GERNWVSPTD VSVERDEKGR 

       610        620        630        640        650        660 
ITKATDNDGN ELIYAGMSKM SKSKNNGIDP QVMVERYGAD TVRLFMMFAS PAEMTLEWQE 

       670        680        690        700        710        720 
SGVEGANRFL KRVWRLAFEH TEKGPTVALD LDALNDEQKA LRRDLHKTIS KVSDDIGRRQ 

       730        740        750        760        770        780 
TFNTAIAAIM ELMNKLARAP QETEQDRALM QEALLAVVRM LNPFTPHASF VLWQALGGAG 

       790        800        810        820        830        840 
EIDNAPWPQA EEAAMVEDSL LVVVQVNGKV RGKITVAADA TQEQVQARAA QEHLVAKYLD 

       850        860 
GVTIRKVIFV PGKLLNLVVG 

« Hide

References

[1]"The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia."
Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R., Geider K.
Environ. Microbiol. 10:2211-2222(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17950 / Et1/99.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU468135 Genomic DNA. Translation: CAO97393.1.
RefSeqYP_001908271.1. NC_010694.1.

3D structure databases

ProteinModelPortalB2VBM4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING465817.ETA_23470.

Proteomic databases

PRIDEB2VBM4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAO97393; CAO97393; ETA_23470.
GeneID6300353.
KEGGeta:ETA_23470.
PATRIC20430559. VBIErwTas9546_2431.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycETAS465817:GI36-2427-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ERWT9
AccessionPrimary (citable) accession number: B2VBM4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries