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B2V9T1

- SYI_SULSY

UniProt

B2V9T1 - SYI_SULSY

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfurihydrogenibium sp. (strain YO3AOP1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
Binding sitei607 – 6071ATPUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi921 – 9211ZincUniRule annotation
Metal bindingi924 – 9241ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSP436114:GI6I-1131-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SYO3AOP1_1087
OrganismiSulfurihydrogenibium sp. (strain YO3AOP1)
Taxonomic identifieri436114 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesHydrogenothermaceaeSulfurihydrogenibium
ProteomesiUP000001201: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 939939Isoleucine--tRNA ligasePRO_1000216246Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi436114.SYO3AOP1_1087.

Structurei

3D structure databases

ProteinModelPortaliB2V9T1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi604 – 6085"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2V9T1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEFKDTLNLP QTEFPMKGNL PNKEPEILSF WEKINLYQKL REDRKGKDKY
60 70 80 90 100
ILHDGPPYAN GHIHIGHALN KILKDILVKY QSMKGKDAPF VPGWDCHGLP
110 120 130 140 150
IEQQVEKELK EKKIKKEDLS KSEFRKLCRE YALKFVNIQK EEFKRLGIIG
160 170 180 190 200
NWEKPYLTMR PSYQAQEVLE LGRVFNKGVA YRGKKPVYWC IYDKTAEAEA
210 220 230 240 250
EIEYYDKKDP SIYVKFKMKD SDDTYLVIWT TTPWTLPANL GVMVHPEFDY
260 270 280 290 300
VYFKTGKGTL IVAKELLENF KEKTGLNGEV IKQVKGKDLE FKEYYHPFID
310 320 330 340 350
RVSKVYLSEF VELGTGTGLV HMAPGHGQED YIIGQRYGVD AFAPVDDEGR
360 370 380 390 400
FIQEAPDWLK GIRVFDANDL IIEKLQEVDA LIYKEVISHS YPHCWRCKNP
410 420 430 440 450
VIFRATPQWF ISMEAKVNEN QTLREAALKE IERVKWIPYY GQNRIKSMVE
460 470 480 490 500
NRPDWCISRQ RSWGVPITVF YCENCGEIVK DMEVFEHVAN LIKNDEFGAD
510 520 530 540 550
IWFEKLVKEL LPEGYKCKKC GGQEFKKEED ILDVWFDSGV SHAAVLKYGE
560 570 580 590 600
WEELRWPADM YLEGSDQHRG WFQSSLLESV ASYNRAPYDT VLTHGFTLDE
610 620 630 640 650
KGRKMSKSAG NVVAPEKVIK EYGADILRLW VVTEDYTEDI KIGFNLIKRI
660 670 680 690 700
AEDYRKIRNT FRYFLGNLYD FNPNQDYVPY ENLLEIDRWM LSKLQNIIQI
710 720 730 740 750
ADKSYEEGKF HKIYHTIKNF VIVDLSAIYL DILKDRLYVY APKSLERKSA
760 770 780 790 800
QTVLWELLLS LNKILAPIIS FTAEEVWQYV RKIDSNIKES IHLEIMPVVN
810 820 830 840 850
EKFIDKNLEE TYEKLLEVRD DILKAIEEAR KQDLVRHPYE ARVILKLPKE
860 870 880 890 900
YKEIVEKRLD WIKFFFTVSQ VELSDNPEGD VVINGESVKD SVIAVSKAKG
910 920 930
EKCPRCWIYD ESVGRNGQPV CDRCKIQLEI MNIKLEELA
Length:939
Mass (Da):109,922
Last modified:July 1, 2008 - v1
Checksum:i321C86AB3AFA3007
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001080 Genomic DNA. Translation: ACD66704.1.
RefSeqiWP_012459771.1. NC_010730.1.
YP_001931258.1. NC_010730.1.

Genome annotation databases

EnsemblBacteriaiACD66704; ACD66704; SYO3AOP1_1087.
GeneIDi6332096.
KEGGisul:SYO3AOP1_1087.
PATRICi23767009. VBISulSp94719_1135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001080 Genomic DNA. Translation: ACD66704.1 .
RefSeqi WP_012459771.1. NC_010730.1.
YP_001931258.1. NC_010730.1.

3D structure databases

ProteinModelPortali B2V9T1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 436114.SYO3AOP1_1087.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD66704 ; ACD66704 ; SYO3AOP1_1087 .
GeneIDi 6332096.
KEGGi sul:SYO3AOP1_1087.
PATRICi 23767009. VBISulSp94719_1135.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SSP436114:GI6I-1131-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YO3AOP1.

Entry informationi

Entry nameiSYI_SULSY
AccessioniPrimary (citable) accession number: B2V9T1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 1, 2008
Last modified: October 29, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3