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B2V9T1

- SYI_SULSY

UniProt

B2V9T1 - SYI_SULSY

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfurihydrogenibium sp. (strain YO3AOP1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
    Binding sitei607 – 6071ATPUniRule annotation
    Metal bindingi903 – 9031ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSSP436114:GI6I-1131-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:SYO3AOP1_1087
    OrganismiSulfurihydrogenibium sp. (strain YO3AOP1)
    Taxonomic identifieri436114 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesHydrogenothermaceaeSulfurihydrogenibium
    ProteomesiUP000001201: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 939939Isoleucine--tRNA ligasePRO_1000216246Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi436114.SYO3AOP1_1087.

    Structurei

    3D structure databases

    ProteinModelPortaliB2V9T1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi604 – 6085"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2V9T1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFKDTLNLP QTEFPMKGNL PNKEPEILSF WEKINLYQKL REDRKGKDKY    50
    ILHDGPPYAN GHIHIGHALN KILKDILVKY QSMKGKDAPF VPGWDCHGLP 100
    IEQQVEKELK EKKIKKEDLS KSEFRKLCRE YALKFVNIQK EEFKRLGIIG 150
    NWEKPYLTMR PSYQAQEVLE LGRVFNKGVA YRGKKPVYWC IYDKTAEAEA 200
    EIEYYDKKDP SIYVKFKMKD SDDTYLVIWT TTPWTLPANL GVMVHPEFDY 250
    VYFKTGKGTL IVAKELLENF KEKTGLNGEV IKQVKGKDLE FKEYYHPFID 300
    RVSKVYLSEF VELGTGTGLV HMAPGHGQED YIIGQRYGVD AFAPVDDEGR 350
    FIQEAPDWLK GIRVFDANDL IIEKLQEVDA LIYKEVISHS YPHCWRCKNP 400
    VIFRATPQWF ISMEAKVNEN QTLREAALKE IERVKWIPYY GQNRIKSMVE 450
    NRPDWCISRQ RSWGVPITVF YCENCGEIVK DMEVFEHVAN LIKNDEFGAD 500
    IWFEKLVKEL LPEGYKCKKC GGQEFKKEED ILDVWFDSGV SHAAVLKYGE 550
    WEELRWPADM YLEGSDQHRG WFQSSLLESV ASYNRAPYDT VLTHGFTLDE 600
    KGRKMSKSAG NVVAPEKVIK EYGADILRLW VVTEDYTEDI KIGFNLIKRI 650
    AEDYRKIRNT FRYFLGNLYD FNPNQDYVPY ENLLEIDRWM LSKLQNIIQI 700
    ADKSYEEGKF HKIYHTIKNF VIVDLSAIYL DILKDRLYVY APKSLERKSA 750
    QTVLWELLLS LNKILAPIIS FTAEEVWQYV RKIDSNIKES IHLEIMPVVN 800
    EKFIDKNLEE TYEKLLEVRD DILKAIEEAR KQDLVRHPYE ARVILKLPKE 850
    YKEIVEKRLD WIKFFFTVSQ VELSDNPEGD VVINGESVKD SVIAVSKAKG 900
    EKCPRCWIYD ESVGRNGQPV CDRCKIQLEI MNIKLEELA 939
    Length:939
    Mass (Da):109,922
    Last modified:July 1, 2008 - v1
    Checksum:i321C86AB3AFA3007
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001080 Genomic DNA. Translation: ACD66704.1.
    RefSeqiWP_012459771.1. NC_010730.1.
    YP_001931258.1. NC_010730.1.

    Genome annotation databases

    EnsemblBacteriaiACD66704; ACD66704; SYO3AOP1_1087.
    GeneIDi6332096.
    KEGGisul:SYO3AOP1_1087.
    PATRICi23767009. VBISulSp94719_1135.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001080 Genomic DNA. Translation: ACD66704.1 .
    RefSeqi WP_012459771.1. NC_010730.1.
    YP_001931258.1. NC_010730.1.

    3D structure databases

    ProteinModelPortali B2V9T1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 436114.SYO3AOP1_1087.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACD66704 ; ACD66704 ; SYO3AOP1_1087 .
    GeneIDi 6332096.
    KEGGi sul:SYO3AOP1_1087.
    PATRICi 23767009. VBISulSp94719_1135.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SSP436114:GI6I-1131-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: YO3AOP1.

    Entry informationi

    Entry nameiSYI_SULSY
    AccessioniPrimary (citable) accession number: B2V9T1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3