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B2V9T1

- SYI_SULSY

UniProt

B2V9T1 - SYI_SULSY

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Protein

Isoleucine--tRNA ligase

Gene
ileS, SYO3AOP1_1087
Organism
Sulfurihydrogenibium sp. (strain YO3AOP1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylate By similarity
Binding sitei607 – 6071ATP By similarity
Metal bindingi903 – 9031Zinc By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi921 – 9211Zinc By similarity
Metal bindingi924 – 9241Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSP436114:GI6I-1131-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SYO3AOP1_1087
OrganismiSulfurihydrogenibium sp. (strain YO3AOP1)
Taxonomic identifieri436114 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesHydrogenothermaceaeSulfurihydrogenibium
ProteomesiUP000001201: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 939939Isoleucine--tRNA ligaseUniRule annotationPRO_1000216246Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi436114.SYO3AOP1_1087.

Structurei

3D structure databases

ProteinModelPortaliB2V9T1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi604 – 6085"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2V9T1-1 [UniParc]FASTAAdd to Basket

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MEFKDTLNLP QTEFPMKGNL PNKEPEILSF WEKINLYQKL REDRKGKDKY    50
ILHDGPPYAN GHIHIGHALN KILKDILVKY QSMKGKDAPF VPGWDCHGLP 100
IEQQVEKELK EKKIKKEDLS KSEFRKLCRE YALKFVNIQK EEFKRLGIIG 150
NWEKPYLTMR PSYQAQEVLE LGRVFNKGVA YRGKKPVYWC IYDKTAEAEA 200
EIEYYDKKDP SIYVKFKMKD SDDTYLVIWT TTPWTLPANL GVMVHPEFDY 250
VYFKTGKGTL IVAKELLENF KEKTGLNGEV IKQVKGKDLE FKEYYHPFID 300
RVSKVYLSEF VELGTGTGLV HMAPGHGQED YIIGQRYGVD AFAPVDDEGR 350
FIQEAPDWLK GIRVFDANDL IIEKLQEVDA LIYKEVISHS YPHCWRCKNP 400
VIFRATPQWF ISMEAKVNEN QTLREAALKE IERVKWIPYY GQNRIKSMVE 450
NRPDWCISRQ RSWGVPITVF YCENCGEIVK DMEVFEHVAN LIKNDEFGAD 500
IWFEKLVKEL LPEGYKCKKC GGQEFKKEED ILDVWFDSGV SHAAVLKYGE 550
WEELRWPADM YLEGSDQHRG WFQSSLLESV ASYNRAPYDT VLTHGFTLDE 600
KGRKMSKSAG NVVAPEKVIK EYGADILRLW VVTEDYTEDI KIGFNLIKRI 650
AEDYRKIRNT FRYFLGNLYD FNPNQDYVPY ENLLEIDRWM LSKLQNIIQI 700
ADKSYEEGKF HKIYHTIKNF VIVDLSAIYL DILKDRLYVY APKSLERKSA 750
QTVLWELLLS LNKILAPIIS FTAEEVWQYV RKIDSNIKES IHLEIMPVVN 800
EKFIDKNLEE TYEKLLEVRD DILKAIEEAR KQDLVRHPYE ARVILKLPKE 850
YKEIVEKRLD WIKFFFTVSQ VELSDNPEGD VVINGESVKD SVIAVSKAKG 900
EKCPRCWIYD ESVGRNGQPV CDRCKIQLEI MNIKLEELA 939
Length:939
Mass (Da):109,922
Last modified:July 1, 2008 - v1
Checksum:i321C86AB3AFA3007
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001080 Genomic DNA. Translation: ACD66704.1.
RefSeqiWP_012459771.1. NC_010730.1.
YP_001931258.1. NC_010730.1.

Genome annotation databases

EnsemblBacteriaiACD66704; ACD66704; SYO3AOP1_1087.
GeneIDi6332096.
KEGGisul:SYO3AOP1_1087.
PATRICi23767009. VBISulSp94719_1135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001080 Genomic DNA. Translation: ACD66704.1 .
RefSeqi WP_012459771.1. NC_010730.1.
YP_001931258.1. NC_010730.1.

3D structure databases

ProteinModelPortali B2V9T1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 436114.SYO3AOP1_1087.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD66704 ; ACD66704 ; SYO3AOP1_1087 .
GeneIDi 6332096.
KEGGi sul:SYO3AOP1_1087.
PATRICi 23767009. VBISulSp94719_1135.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SSP436114:GI6I-1131-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YO3AOP1.

Entry informationi

Entry nameiSYI_SULSY
AccessioniPrimary (citable) accession number: B2V9T1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 1, 2008
Last modified: September 3, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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