B2V7T0 (B2V7T0_SULSY) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ACD66003.1

Gene names

Ordered Locus Names:

SYO3AOP1_0358

Organism

Sulfurihydrogenibium sp. (strain YO3AOP1) [Complete proteome] [HAMAP]

Taxonomic identifier

436114 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Hydrogenothermaceae › Sulfurihydrogenibium

Protein attributes

Sequence length	683 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS005733

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B2V7T0 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 571FE38BC82DA76C	FASTA	683	79,006
	10 20 30 40 50 60 MSEKKIVIVE SPKKAKEISH FLGKDFIVKA TVGHFKDLPE NEMGVDLNTY EPKFVIKSQN 70 80 90 100 110 120 HKKILSEIKK LAEKSEVIIA TDPDREGYAI GFFMYEELKK KAKEVKRAEF HEITKEHIKE 130 140 150 160 170 180 KIKNAVPFEK TNFGLFNAFL GRRVGDRIVG YTLSPIASRE IGGRFSVGRV QSPAVRLIVE 190 200 210 220 230 240 REIEIQNFKP TPYYVLSAVL RATSHVSPLT FTAIYENPKI ENKEEAEKIY ADIKDEKIAT 250 260 270 280 290 300 IKKVEKKQVK QSPKPPFTTS TLQQTASTVY RFAPEKTMML AQDLFESGLI TYHRTDSVRI 310 320 330 340 350 360 SEKAIDGIRN LINKEFGKDY LPEKIRVYKS KNTQADAHEA IRITHFVNLE EQKKLIKEKG 370 380 390 400 410 420 LSEDHFKLLK LIYERTVASQ MADAIFERTS AAVDIKGYTF KASGSILKFK GYKAVYDLEE 430 440 450 460 470 480 EEDKEENQKL PDLKNKDVLT LEKINLEEKF TKPPARYTEG GLVKKLEELG IGRPSTYATI 490 500 510 520 530 540 IKTIKERGYV VKENGSLRPT ENAFHLIEYL NTRYNWVIDY SFTKKMEDFL DKVEENKKDW 550 560 570 580 590 600 KEFVKQLHEK SGSKQNVKIS KKMLDYAKDL AEKHGKSIEE IKDDPEKLKE FIDAHAEKNP 610 620 630 640 650 660 SQKQIEYAKS LAEKTGLKLT EKELLDRKLL TKWINKAKKE MMKNYQLSEK QKNVLIKYGR 670 680 EDLVNNPVQA LKFIKSKLSR YKK

References

[1]

"Complete and draft genome sequences of six members of the Aquificales." Reysenbach A.-L., Hamamura N., Podar M., Griffiths E., Ferreira S., Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A., Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A. J. Bacteriol. 191:1992-1993(2009) [PubMed: 19136599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001080 Genomic DNA. Translation: ACD66003.1.
RefSeq	YP_001930557.1. NC_010730.1.
3D structure databases
ProteinModelPortal	B2V7T0.
ModBase	Search...
Protein-protein interaction databases
STRING	B2V7T0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6331713.
GenomeReviews	Gene locus SYO3AOP1_0358 in contig CP001080_GR.
KEGG	sul:SYO3AOP1_0358.
PATRIC	23765435. VBISulSp94719_0371.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	QELDETC.
ProtClustDB	CLSK2479394.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B2V7T0_SULSY

Accession

Primary (citable) accession number: B2V7T0

Entry history

Integrated into UniProtKB/TrEMBL:	July 1, 2008
Last sequence update:	July 1, 2008
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)