B2V7G7 (MTNC_SULSY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enolase-phosphatase E1
EC=3.1.3.77

Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphatase

Gene names

Name:	mtnC
Ordered Locus Names:	SYO3AOP1_0245

Organism

Sulfurihydrogenibium sp. (strain YO3AOP1) [Complete proteome] [HAMAP]

Taxonomic identifier

436114 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Hydrogenothermaceae › Sulfurihydrogenibium

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity. HAMAP MF_01681
Catalytic activity	5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate. HAMAP MF_01681
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01681
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. HAMAP MF_01681 Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Subunit structure	Monomer By similarity. HAMAP MF_01681
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: InterPro
Molecular function	2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity Inferred from electronic annotation. Source: InterPro 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity Inferred from electronic annotation. Source: InterPro acireductone synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro phosphoglycolate phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Enolase-phosphatase E1 HAMAP MF_01681		PRO_0000357417

Sequences

	Sequence		Length	Mass (Da)
	B2V7G7 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 4648B2BDBE8F39A7	FASTA	230	26,291
	10 20 30 40 50 60 MIKAILTDIE GTTTPISFVK DVLFPYSYEK IEEFVKNNLQ NPQVQKIIED VKKEINKSDA 70 80 90 100 110 120 SLEEVIENLK SWIVEDKKIT PLKELQGLIW EEGYKSGKLQ GFVYPDAYNK LKEWFDSGIK 130 140 150 160 170 180 IFIYSSGSVK AQKLLFSNTN YGDLNYLFSG YFDTNIGNKK DKQSYVKIAK EIGFSPSEIL 190 200 210 220 230 FLSDNPDEII AAASAGYNVI RLVRPLDADH IDNFPYKQVE SFDEIEIGRV

References

[1]

"Complete and draft genome sequences of six members of the Aquificales." Reysenbach A.-L., Hamamura N., Podar M., Griffiths E., Ferreira S., Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A., Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A. J. Bacteriol. 191:1992-1993(2009) [PubMed: 19136599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YO3AOP1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001080 Genomic DNA. Translation: ACD65890.1.
RefSeq	YP_001930444.1. NC_010730.1.
3D structure databases
ProteinModelPortal	B2V7G7.
ModBase	Search...
Protein-protein interaction databases
STRING	B2V7G7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6331185.
GenomeReviews	Gene locus SYO3AOP1_0245 in contig CP001080_GR.
KEGG	sul:SYO3AOP1_0245.
PATRIC	23765185. VBISulSp94719_0257.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG294242.
OMA	TTDLNFI.
ProtClustDB	CLSK866737.
Family and domain databases
HAMAP	MF_01681. Salvage_MtnC. [Tree]
InterPro	IPR005834. Dehalogen-like_hydro. IPR023943. Enolase-phosphatase_E1. IPR010041. Enolase_ppase. IPR023214. HAD-like_dom. IPR006439. HAD-SF_hydro_IA_v1. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
KO	K09880.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01691. Enolase-ppase. 1 hit. TIGR01549. HAD-SF-IA-v1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNC_SULSY

Accession

Primary (citable) accession number: B2V7G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program