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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Sulfurihydrogenibium sp. (strain YO3AOP1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei251 – 2511ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSP436114:GI6I-1373-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:SYO3AOP1_1325
OrganismiSulfurihydrogenibium sp. (strain YO3AOP1)
Taxonomic identifieri436114 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesHydrogenothermaceaeSulfurihydrogenibium
ProteomesiUP000001201 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478Glutamate--tRNA ligasePRO_0000367776Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi436114.SYO3AOP1_1325.

Structurei

3D structure databases

ProteinModelPortaliB2V5J1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi8 – 1811"HIGH" regionAdd
BLAST
Motifi248 – 2525"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiAFRCFCT.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2V5J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRVRFAPSP TGYLHLGNAR TALFNYLFSR HENATFILRI EDTDLERSKK
60 70 80 90 100
EYEEMLMEDL KWMGIEWDEG PDAGGPHGPY RQSERLEIYM KYVDKLLKSG
110 120 130 140 150
DAYYCYCTEE ELEQEREKAI AEGRPYRYSG KCRNLTPEER AFYEGKSIKP
160 170 180 190 200
VIRFKVPDKT VVFEDIIRGH VEIDTKEFGD FVIVRQDGMP VYNFVVVIDD
210 220 230 240 250
ALMGITHVIR GEDHLSNTPK QIVIYEALGF AIPQFAHLPI ILGEDRTKLS
260 270 280 290 300
KRHGAVSVRA LKDDGFISEA VFNYLSLLGW HPKEEKEILS KEEIIKQFRI
310 320 330 340 350
EDVNKSPAIF DRTKLRWMNG VYIREILDLD DLTKRAIPFF EGFGYKADYE
360 370 380 390 400
FYKKVMSAIR DSIETLMEIK ERAKVFFVDE FPYTEEIVNE VKSDENVYKV
410 420 430 440 450
VEIFYNKIKN LSAITKEDFK NITKEIQKEY GYKGKALFHP IRIALTGEPS
460 470
GVGLDLLVEV IGIERVKFRL ERFLEYFG
Length:478
Mass (Da):55,797
Last modified:July 1, 2008 - v1
Checksum:i9DE44E847E45BD25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001080 Genomic DNA. Translation: ACD66935.1.
RefSeqiWP_012459994.1. NC_010730.1.
YP_001931489.1. NC_010730.1.

Genome annotation databases

EnsemblBacteriaiACD66935; ACD66935; SYO3AOP1_1325.
KEGGisul:SYO3AOP1_1325.
PATRICi23767513. VBISulSp94719_1382.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001080 Genomic DNA. Translation: ACD66935.1.
RefSeqiWP_012459994.1. NC_010730.1.
YP_001931489.1. NC_010730.1.

3D structure databases

ProteinModelPortaliB2V5J1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi436114.SYO3AOP1_1325.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD66935; ACD66935; SYO3AOP1_1325.
KEGGisul:SYO3AOP1_1325.
PATRICi23767513. VBISulSp94719_1382.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiAFRCFCT.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciSSP436114:GI6I-1373-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YO3AOP1.

Entry informationi

Entry nameiSYE_SULSY
AccessioniPrimary (citable) accession number: B2V5J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: April 1, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.