Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Clostridium botulinum (strain Alaska E43 / Type E3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109ZincUniRule annotation1
Metal bindingi111ZincUniRule annotation1
Metal bindingi136ZincUniRule annotation1
Metal bindingi138ZincUniRule annotation1
Binding sitei256ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:CLH_2322
OrganismiClostridium botulinum (strain Alaska E43 / Type E3)
Taxonomic identifieri508767 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003676481 – 485Glutamate--tRNA ligaseAdd BLAST485

Proteomic databases

PRIDEiB2V5H4.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB2V5H4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 22"HIGH" regionUniRule annotationAdd BLAST11
Motifi253 – 257"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252720.
KOiK01885.
OMAiLKTVEWT.
OrthoDBiPOG0918009A.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

B2V5H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKKIRTRF APSPTGYMHV GNLRTALYAY LIAKHEAGDF ILRIEDTDQE
60 70 80 90 100
RLVDGAVDII YNTLKLTGLN HDEGPDVGGN VGPYVQSERK AIYLEYAKNL
110 120 130 140 150
VEKGEAYYCF CSKDRLDMLK ENAEALKRPF KYDKHCLHLT KEEIEANLAK
160 170 180 190 200
GLPYVIRQNN PTTGSTTFDD VIYGKITVDN SELEDMILIK SDGLPTYNFA
210 220 230 240 250
NVVDDHLMGI THIVRGNEYL SSSPKYNRLY EAFGWDIPVY VHCPPIMKDT
260 270 280 290 300
HNKLSKRNGD ASFEDLMKKG YLKDAVLNYI ALLGWNPGTN EEIFNLEELT
310 320 330 340 350
QKFDFKDISK SPAIFDDAKL KWMNGEYIRK LSLDEFHELA VPEYKKVLKK
360 370 380 390 400
DFDLKFISEL LHTRCELLSD LAEQIDFLEE LPEYSTDLYV HKKMKSTVES
410 420 430 440 450
SLENLQKVLP IIEEIDEANW NKDYIHEKVF ELIKSLEIKN GQMLWPIRTA
460 470 480
LSGKSFTPGG AFELAILLGK EESISRLKKG IELLK
Length:485
Mass (Da):55,581
Last modified:July 1, 2008 - v1
Checksum:i65A3A8694DE15970
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001078 Genomic DNA. Translation: ACD52707.1.
RefSeqiWP_012450792.1. NC_010723.1.

Genome annotation databases

EnsemblBacteriaiACD52707; ACD52707; CLH_2322.
KEGGicbt:CLH_2322.
PATRICi19420901. VBICloBot115804_2242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001078 Genomic DNA. Translation: ACD52707.1.
RefSeqiWP_012450792.1. NC_010723.1.

3D structure databases

ProteinModelPortaliB2V5H4.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiB2V5H4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD52707; ACD52707; CLH_2322.
KEGGicbt:CLH_2322.
PATRICi19420901. VBICloBot115804_2242.

Phylogenomic databases

HOGENOMiHOG000252720.
KOiK01885.
OMAiLKTVEWT.
OrthoDBiPOG0918009A.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_CLOBA
AccessioniPrimary (citable) accession number: B2V5H4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: February 15, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.