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B2V5H4

- SYE_CLOBA

UniProt

B2V5H4 - SYE_CLOBA

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Clostridium botulinum (strain Alaska E43 / Type E3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi109 – 1091ZincUniRule annotation
    Metal bindingi111 – 1111ZincUniRule annotation
    Metal bindingi136 – 1361ZincUniRule annotation
    Metal bindingi138 – 1381ZincUniRule annotation
    Binding sitei256 – 2561ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCBOT508767:GHKO-2314-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:CLH_2322
    OrganismiClostridium botulinum (strain Alaska E43 / Type E3)
    Taxonomic identifieri508767 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001738: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 485485Glutamate--tRNA ligasePRO_0000367648Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi508767.CLH_2322.

    Structurei

    3D structure databases

    ProteinModelPortaliB2V5H4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi12 – 2211"HIGH" regionAdd
    BLAST
    Motifi253 – 2575"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    KOiK01885.
    OMAiDSHEHHA.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2V5H4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASKKIRTRF APSPTGYMHV GNLRTALYAY LIAKHEAGDF ILRIEDTDQE    50
    RLVDGAVDII YNTLKLTGLN HDEGPDVGGN VGPYVQSERK AIYLEYAKNL 100
    VEKGEAYYCF CSKDRLDMLK ENAEALKRPF KYDKHCLHLT KEEIEANLAK 150
    GLPYVIRQNN PTTGSTTFDD VIYGKITVDN SELEDMILIK SDGLPTYNFA 200
    NVVDDHLMGI THIVRGNEYL SSSPKYNRLY EAFGWDIPVY VHCPPIMKDT 250
    HNKLSKRNGD ASFEDLMKKG YLKDAVLNYI ALLGWNPGTN EEIFNLEELT 300
    QKFDFKDISK SPAIFDDAKL KWMNGEYIRK LSLDEFHELA VPEYKKVLKK 350
    DFDLKFISEL LHTRCELLSD LAEQIDFLEE LPEYSTDLYV HKKMKSTVES 400
    SLENLQKVLP IIEEIDEANW NKDYIHEKVF ELIKSLEIKN GQMLWPIRTA 450
    LSGKSFTPGG AFELAILLGK EESISRLKKG IELLK 485
    Length:485
    Mass (Da):55,581
    Last modified:July 1, 2008 - v1
    Checksum:i65A3A8694DE15970
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001078 Genomic DNA. Translation: ACD52707.1.
    RefSeqiWP_012450792.1. NC_010723.1.
    YP_001921703.1. NC_010723.1.

    Genome annotation databases

    EnsemblBacteriaiACD52707; ACD52707; CLH_2322.
    GeneIDi6318920.
    KEGGicbt:CLH_2322.
    PATRICi19420901. VBICloBot115804_2242.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001078 Genomic DNA. Translation: ACD52707.1 .
    RefSeqi WP_012450792.1. NC_010723.1.
    YP_001921703.1. NC_010723.1.

    3D structure databases

    ProteinModelPortali B2V5H4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 508767.CLH_2322.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACD52707 ; ACD52707 ; CLH_2322 .
    GeneIDi 6318920.
    KEGGi cbt:CLH_2322.
    PATRICi 19420901. VBICloBot115804_2242.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    KOi K01885.
    OMAi DSHEHHA.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci CBOT508767:GHKO-2314-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Clostridium botulinum E3 str. Alaska E43."
      Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
      Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Alaska E43 / Type E3.

    Entry informationi

    Entry nameiSYE_CLOBA
    AccessioniPrimary (citable) accession number: B2V5H4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3