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Protein

Catalase-peroxidase

Gene

katG

Organism
Clostridium botulinum (strain Alaska E43 / Type E3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei92Transition state stabilizerUniRule annotation1
Active sitei96Proton acceptorUniRule annotation1
Metal bindingi259Iron (heme axial ligand)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Ordered Locus Names:CLH_1313
OrganismiClostridium botulinum (strain Alaska E43 / Type E3)
Taxonomic identifieri508767 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003547631 – 730Catalase-peroxidaseAdd BLAST730

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki95 ↔ 218Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-244)UniRule annotation
Cross-linki218 ↔ 244Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-95)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Proteomic databases

PRIDEiB2V515.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OMAiIAEVYAC.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2V515-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTENKCPVTG KMSKATAGSG TTNKDWWPNQ LNLNILHQNS QLSNPMSKDF
60 70 80 90 100
NYAEEFKKLD FQALKVDLYM LMTDSQIWWP ADYGNYGPFF IRMAWHSAGT
110 120 130 140 150
YRVGDGRGGG SFGLQRFAPL NSWPDNINLD KARRLLWPIK KKYGNKISWA
160 170 180 190 200
DLMILTGNCA LESMGLKTLG FGGGRVDVWE PQEDIYWGSE KEWLGDEREK
210 220 230 240 250
DDEELENPLA AVQMGLIYVN PEGPNGNPDP LGSAHDVRET FARMAMNDEE
260 270 280 290 300
TVALVAGGHT FGKCHGAASP SYVGPAPEAA PIEEQGLGWK NTYGSGNGDD
310 320 330 340 350
TIGSGLEGAW KANPTKWTMG YLKTLFKYDW ELVKSPAGAY QWLAKNVDEE
360 370 380 390 400
DMVIDAEDST KKHRPMMTTA DLGLRYDPIY EPIARNYLKN PEKFAHDFAA
410 420 430 440 450
AWFKLTHRDM GPISRYLGPE VPKESFIWQD PIPLVKHKLI TKKDITHIKK
460 470 480 490 500
KILDSGLSIS DLVATAWASA STFRGSDKRG GANGARIRLE PQKNWEVNEP
510 520 530 540 550
KKLNNVLNTL KQIKENFNSY HSKDKKVSLA DIIILGGCVG IEQAAKRAGY
560 570 580 590 600
NINVPFIPGR TDATQKQTDV KSFAVLEPKG DGFRNYLKTK YVVKPEDMLI
610 620 630 640 650
DRAQLLTLTA PEMTVLIGGM RVLNCNYNKS KDGVFTNRPE CLTNDFFVNL
660 670 680 690 700
LDMNTVWKPK SEGKDRFEGF DRETGELKWT ATRVDLIFGS NSQLRAIAEV
710 720 730
YACDDNKEKF IQDFIFAWNK IMNADRFEIK
Length:730
Mass (Da):82,181
Last modified:July 1, 2008 - v1
Checksum:i47B8753352644F0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001078 Genomic DNA. Translation: ACD52530.1.

Genome annotation databases

EnsemblBacteriaiACD52530; ACD52530; CLH_1313.
KEGGicbt:CLH_1313.
PATRICi19418894. VBICloBot115804_1244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001078 Genomic DNA. Translation: ACD52530.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiB2V515.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD52530; ACD52530; CLH_1313.
KEGGicbt:CLH_1313.
PATRICi19418894. VBICloBot115804_1244.

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OMAiIAEVYAC.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKATG_CLOBA
AccessioniPrimary (citable) accession number: B2V515
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: July 1, 2008
Last modified: November 2, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.