B2V515 (KATG_CLOBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	CLH_1313

Organism

Clostridium botulinum (strain Alaska E43 / Type E3) [Complete proteome] [HAMAP]

Taxonomic identifier

508767 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	730 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 730	730	Catalase-peroxidase HAMAP MF_01961		PRO_0000354763
Sites
	Active site	96	1	Proton acceptor By similarity
	Metal binding	259	1	Iron (heme axial ligand) By similarity
	Site	92	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	95 ↔ 218		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-244) By similarity
	Cross-link	218 ↔ 244		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-95) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2V515 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 47B8753352644F0A	FASTA	730	82,181
	10 20 30 40 50 60 MTENKCPVTG KMSKATAGSG TTNKDWWPNQ LNLNILHQNS QLSNPMSKDF NYAEEFKKLD 70 80 90 100 110 120 FQALKVDLYM LMTDSQIWWP ADYGNYGPFF IRMAWHSAGT YRVGDGRGGG SFGLQRFAPL 130 140 150 160 170 180 NSWPDNINLD KARRLLWPIK KKYGNKISWA DLMILTGNCA LESMGLKTLG FGGGRVDVWE 190 200 210 220 230 240 PQEDIYWGSE KEWLGDEREK DDEELENPLA AVQMGLIYVN PEGPNGNPDP LGSAHDVRET 250 260 270 280 290 300 FARMAMNDEE TVALVAGGHT FGKCHGAASP SYVGPAPEAA PIEEQGLGWK NTYGSGNGDD 310 320 330 340 350 360 TIGSGLEGAW KANPTKWTMG YLKTLFKYDW ELVKSPAGAY QWLAKNVDEE DMVIDAEDST 370 380 390 400 410 420 KKHRPMMTTA DLGLRYDPIY EPIARNYLKN PEKFAHDFAA AWFKLTHRDM GPISRYLGPE 430 440 450 460 470 480 VPKESFIWQD PIPLVKHKLI TKKDITHIKK KILDSGLSIS DLVATAWASA STFRGSDKRG 490 500 510 520 530 540 GANGARIRLE PQKNWEVNEP KKLNNVLNTL KQIKENFNSY HSKDKKVSLA DIIILGGCVG 550 560 570 580 590 600 IEQAAKRAGY NINVPFIPGR TDATQKQTDV KSFAVLEPKG DGFRNYLKTK YVVKPEDMLI 610 620 630 640 650 660 DRAQLLTLTA PEMTVLIGGM RVLNCNYNKS KDGVFTNRPE CLTNDFFVNL LDMNTVWKPK 670 680 690 700 710 720 SEGKDRFEGF DRETGELKWT ATRVDLIFGS NSQLRAIAEV YACDDNKEKF IQDFIFAWNK 730 IMNADRFEIK

References

[1]

"Complete genome sequence of Clostridium botulinum E3 str. Alaska E43." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Alaska E43 / Type E3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001078 Genomic DNA. Translation: ACD52530.1.
RefSeq	YP_001920707.1. NC_010723.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2V515.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6319321.
GenomeReviews	Gene locus CLH_1313 in contig CP001078_GR.
KEGG	cbt:CLH_1313.
PATRIC	19418894. VBICloBot115804_1244.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_CLOBA

Accession

Primary (citable) accession number: B2V515

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program