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Protein

Ribosomal RNA small subunit methyltransferase H

Gene

rsmH

Organism
Clostridium botulinum (strain Alaska E43 / Type E3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531S-adenosyl-L-methionineUniRule annotation
Binding sitei79 – 791S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei100 – 1001S-adenosyl-L-methionineUniRule annotation
Binding sitei107 – 1071S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBOT508767:GHKO-2205-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase HUniRule annotation (EC:2.1.1.199UniRule annotation)
Alternative name(s):
16S rRNA m(4)C1402 methyltransferaseUniRule annotation
rRNA (cytosine-N(4)-)-methyltransferase RsmHUniRule annotation
Gene namesi
Name:rsmHUniRule annotation
Synonyms:mraW
Ordered Locus Names:CLH_2213
OrganismiClostridium botulinum (strain Alaska E43 / Type E3)
Taxonomic identifieri508767 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Ribosomal RNA small subunit methyltransferase HPRO_0000386811Add
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 353S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RsmH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000049777.
KOiK03438.
OMAiHVPVMLQ.
OrthoDBiPOG091H02LX.

Family and domain databases

Gene3Di1.10.150.170. 1 hit.
3.40.50.150. 2 hits.
HAMAPiMF_01007. 16SrRNA_methyltr_H. 1 hit.
InterProiIPR002903. RsmH.
IPR023397. SAM-dep_MeTrfase_MraW_recog.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11265. PTHR11265. 1 hit.
PfamiPF01795. Methyltransf_5. 1 hit.
[Graphical view]
PIRSFiPIRSF004486. MraW. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
SSF81799. SSF81799. 1 hit.
TIGRFAMsiTIGR00006. TIGR00006. 1 hit.

Sequencei

Sequence statusi: Complete.

B2V4W0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFKHISVLL NECLDALDIK DNGIYVDCTL GGAGHSSHIL ERLSNEGLLI
60 70 80 90 100
GIDQDRDALK AAKERLKRFE NVKYVHSNFY DIDNILQNLD IPKVDGILMD
110 120 130 140 150
LGVSSYQLDE GARGFSYMKD APLDMRMNRD NDFSAYEIVN EYSEDELYKI
160 170 180 190 200
IRNYGEERFA KRISNCIVNR RSDKPIETTM ELVDIIKAAI PAKARREGPH
210 220 230 240 250
PAKRTFQAIR IEVNSELKIL NQTIEDGVNR LKPGGRMAII TFHSLEDRIV
260 270 280 290 300
KLKFRELNDP CTCPREFPMC ICGKKPSVRL VSRKGIEPTK EEVEENPRSR
310
SAKLRIIEKL
Length:310
Mass (Da):35,514
Last modified:July 1, 2008 - v1
Checksum:i82DA5D0681DDB785
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001078 Genomic DNA. Translation: ACD51171.1.
RefSeqiWP_003369055.1. NC_010723.1.

Genome annotation databases

EnsemblBacteriaiACD51171; ACD51171; CLH_2213.
KEGGicbt:CLH_2213.
PATRICi19420685. VBICloBot115804_2134.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001078 Genomic DNA. Translation: ACD51171.1.
RefSeqiWP_003369055.1. NC_010723.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD51171; ACD51171; CLH_2213.
KEGGicbt:CLH_2213.
PATRICi19420685. VBICloBot115804_2134.

Phylogenomic databases

HOGENOMiHOG000049777.
KOiK03438.
OMAiHVPVMLQ.
OrthoDBiPOG091H02LX.

Enzyme and pathway databases

BioCyciCBOT508767:GHKO-2205-MONOMER.

Family and domain databases

Gene3Di1.10.150.170. 1 hit.
3.40.50.150. 2 hits.
HAMAPiMF_01007. 16SrRNA_methyltr_H. 1 hit.
InterProiIPR002903. RsmH.
IPR023397. SAM-dep_MeTrfase_MraW_recog.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11265. PTHR11265. 1 hit.
PfamiPF01795. Methyltransf_5. 1 hit.
[Graphical view]
PIRSFiPIRSF004486. MraW. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
SSF81799. SSF81799. 1 hit.
TIGRFAMsiTIGR00006. TIGR00006. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSMH_CLOBA
AccessioniPrimary (citable) accession number: B2V4W0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: July 1, 2008
Last modified: September 7, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.