B2V4R1 (PPNK_CLOBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	CLH_2164

Organism

Clostridium botulinum (strain Alaska E43 / Type E3) [Complete proteome] [HAMAP]

Taxonomic identifier

508767 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium botulinum › Clostridium botulinum E

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP-Rule MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361
Cofactor	Divalent metal ions By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	NAD metabolic process Inferred from electronic annotation. Source: InterPro NADP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP-Rule MF_00361		PRO_1000120844

Sequences

	Sequence		Length	Mass (Da)
	B2V4R1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 7D1193D89465979E	FASTA	284	31,404
	10 20 30 40 50 60 MKNIGIAINP SKDYKNTILN MVKEKIENIC NVTDIEVYNS FDIKNLNLSS LDLLIVLGGD 70 80 90 100 110 120 GTLLGVAREL EDDFKAPILG INIGNLGVLS SIEISDLELA LKKLMTKDCK VHKRMMLNCE 130 140 150 160 170 180 VDINESIKNI KALNEVAVAR GTLSRMVKFK IFVDEKLYAI FKGDGLIVST PTGSTAYSFS 190 200 210 220 230 240 AGGPFICPDL EVISIVPICD HTKSMHPIVL KGDSTIKIIA ENGGDQIYLT IDGQRAIEMK 250 260 270 280 DNSVITVKKN PKSLKLLLFN DYDYFKVIRN KVLNNSKECD GEEI

References

[1]

"Complete genome sequence of Clostridium botulinum E3 str. Alaska E43." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Alaska E43 / Type E3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001078 Genomic DNA. Translation: ACD53604.1.
RefSeq	YP_001921545.1. NC_010723.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	508767.CLH_2164.
Proteomic databases
PRIDE	B2V4R1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD53604; ACD53604; CLH_2164.
GeneID	6320180.
KEGG	cbt:CLH_2164.
PATRIC	19420587. VBICloBot115804_2085.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0061.
HOGENOM	HOG000227222.
KO	K00858.
OMA	FANPDQH.
ProtClustDB	CLSK811135.
Enzyme and pathway databases
BioCyc	CBOT508767:GHKO-2654-MONOMER.
Family and domain databases
Gene3D	2.60.200.30. 1 hit. 3.40.50.10330. 1 hit.
HAMAP	MF_00361. NAD_kinase.
InterPro	IPR017438. ATP-NAD_kinase_dom_1. IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. PolyP/ATP_NADK_prd. [Graphical view]
PANTHER	PTHR20275. PTHR20275. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPNK_CLOBA

Accession

Primary (citable) accession number: B2V4R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program