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Protein

Inosose dehydratase

Gene

iolE

Organism
Clostridium botulinum (strain Alaska E43 / Type E3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the dehydration of inosose (2-keto-myo-inositol, 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi-inositol).UniRule annotation

Catalytic activityi

2,4,6/3,5-pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • glutathioneUniRule annotation
  • Co2+UniRule annotation, Mn2+UniRule annotation

Pathwayi: myo-inositol degradation into acetyl-CoA

This protein is involved in step 2 of the subpathway that synthesizes acetyl-CoA from myo-inositol.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Inosose dehydratase (iolE)
  3. 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (iolD)
  4. no protein annotated in this organism
  5. 5-dehydro-2-deoxygluconokinase (iolC)
  6. no protein annotated in this organism
  7. no protein annotated in this organism
This subpathway is part of the pathway myo-inositol degradation into acetyl-CoA, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from myo-inositol, the pathway myo-inositol degradation into acetyl-CoA and in Polyol metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Cobalt, Manganese

Enzyme and pathway databases

BioCyciCBOT508767:GHKO-1253-MONOMER.
UniPathwayiUPA00076; UER00144.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosose dehydrataseUniRule annotation (EC:4.2.1.44UniRule annotation)
Alternative name(s):
2-keto-myo-inositol dehydrataseUniRule annotation
Short name:
2KMI dehydrataseUniRule annotation
Gene namesi
Name:iolEUniRule annotation
Ordered Locus Names:CLH_1257
OrganismiClostridium botulinum (strain Alaska E43 / Type E3)
Taxonomic identifieri508767 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Inosose dehydratasePRO_0000352362Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB2V4K2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IolE/MocC family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000239720.
KOiK03335.
OMAiHPLTYAT.
OrthoDBiPOG091H0BG2.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_01672. IolE. 1 hit.
InterProiIPR023952. IolE.
IPR030823. IolE/MocC.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR12110:SF4. PTHR12110:SF4. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR04379. myo_inos_iolE. 1 hit.

Sequencei

Sequence statusi: Complete.

B2V4K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNSDKVKIG ICPIGWTNDD MPDLGKENTF EQAISEMALA GFKGTEIGNK
60 70 80 90 100
YPKDVKVLKK ALEMRNLQIA SAWFSSFLTT KPYEETEKEF IAYRDFLHAM
110 120 130 140 150
GSKVIVVSEQ GHSIQGQMET PIFDGKYHFN EEEWNLLANG LNKLGQLAAD
160 170 180 190 200
KGMKIVYHHH MGTGVQTTEE IDKLMSVTDE NLVYLLFDTG HLVYSGENPV
210 220 230 240 250
EILKKYVHRI KHVHLKDIRP EIVSKVKNEK LSFLKGVRAG AFTVPGDGSI
260 270 280 290
DFEPIFKILA ENNYEGWLMI EAEQDPSIAN PLEYAIKGRQ YIKEKASI
Length:298
Mass (Da):33,762
Last modified:July 1, 2008 - v1
Checksum:i0800E502D7098623
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001078 Genomic DNA. Translation: ACD53876.1.
RefSeqiWP_012451693.1. NC_010723.1.

Genome annotation databases

EnsemblBacteriaiACD53876; ACD53876; CLH_1257.
KEGGicbt:CLH_1257.
PATRICi19418778. VBICloBot115804_1186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001078 Genomic DNA. Translation: ACD53876.1.
RefSeqiWP_012451693.1. NC_010723.1.

3D structure databases

ProteinModelPortaliB2V4K2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD53876; ACD53876; CLH_1257.
KEGGicbt:CLH_1257.
PATRICi19418778. VBICloBot115804_1186.

Phylogenomic databases

HOGENOMiHOG000239720.
KOiK03335.
OMAiHPLTYAT.
OrthoDBiPOG091H0BG2.

Enzyme and pathway databases

UniPathwayiUPA00076; UER00144.
BioCyciCBOT508767:GHKO-1253-MONOMER.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_01672. IolE. 1 hit.
InterProiIPR023952. IolE.
IPR030823. IolE/MocC.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR12110:SF4. PTHR12110:SF4. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR04379. myo_inos_iolE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIOLE_CLOBA
AccessioniPrimary (citable) accession number: B2V4K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: July 1, 2008
Last modified: September 7, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.