Reviewed, UniProtKB/Swiss-Prot B2V4K0 (IOLD_CLOBA)

Names and origin

Protein names

Recommended name:
    3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase
      Short name=THcHDO hydrolase
    EC=3.7.1.n2

Gene names

Name:	iolD
Ordered Locus Names:	CLH_1255

Organism

Clostridium botulinum (strain Alaska E43 / Type E3) [Complete proteome] [HAMAP]

Taxonomic identifier

508767 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the cleavage of the C1-C2 bond of 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy-glucuronate (5DG) By similarity.
Catalytic activity	3,5/4-trihydroxycyclohexa-1,2-dione + H2O = 5-deoxy-glucuronic acid. HAMAP MF_01669
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 3/7. HAMAP MF_01669
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding NAD Thiamine pyrophosphate
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: HAMAP thiamin pyrophosphate binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 643	643	3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase HAMAP MF_01669		PRO_0000352537
Regions
	Region	441 – 521	81	Thiamine pyrophosphate binding By similarity
Sites
	Metal binding	492	1	Magnesium By similarity
	Metal binding	519	1	Magnesium By similarity
	Binding site	65	1	Thiamine pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2V4K0-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 9FBA9241B930D859	FASTA	643	71,489
	10 20 30 40 50 60 MKSTKMTTAQ ALVRFLDNQY VSFDGKEEKF VHGIFTIFGH GIVVGLGQAL DENTRDLKVY 70 80 90 100 110 120 QGRNEQGMAH AATAFAKQNN RRKIIACSSS IGPGAANMVT AAATATVNNI PLLLLPGDSF 130 140 150 160 170 180 ATRQPDPVLQ QIEQSYNLGI TTNDVFKPVC KYWDRVNRPE QLMSAMINAM RVLTDPAETG 190 200 210 220 230 240 AVCISLPQDV QGESFEFPEY FFKKRVHKIT RPLAVEEEFY ECLNIIKNKK KPIIICGGGV 250 260 270 280 290 300 RYSEAGDALS KFANKFNIPI GETQAGKSSI KSSDPMNLGG IGVTGNLASN IIAKDADLVI 310 320 330 340 350 360 GVGTRFSDFT TASKSLFKNP DVEFVTINLS KFHASKLDSC KMVGDAKECL EYLHKLLEKE 370 380 390 400 410 420 NYISSYKDEI KDAKIAWKEE MKRLTNIKYE ENFEPIIKFR NKESLEEFKK LTDTTITQTS 430 440 450 460 470 480 ALGLIRECID DDSIIVGASG SLPGDLQRMW ETESLNSYHM EYGYSCMGYE IAAGFGAKLA 490 500 510 520 530 540 DPEKEVYSIL GDGSYLMLHS ELITSIQENK KVNVLLFDNC GFGCINNLQM SNGIGNLATE 550 560 570 580 590 600 FRYRNSETNK LNGKLIPIDF AKAAEGYGLK TYTAKNLEEL KNALIDAKKQ KVSTLIDIKV 610 620 630 640 LPKTMTDGYE SWWHVGLAEV SEKESVNEAF KNSKKILKGA RKY

References

[1]	"Complete genome sequence of Clostridium botulinum E3 str. Alaska E43." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001078 Genomic DNA. Translation: ACD52572.1.
RefSeq	YP_001920649.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6320314.
GenomeReviews	Gene locus CLH_1255 in contig CP001078_GR.
KEGG	cbt:CLH_1255.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B2V4K0. KYLSWWR.
Family and domain databases
HAMAP	MF_01669. [Tree]
InterPro	IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLD_CLOBA

Accession

Primary (citable) accession number: B2V4K0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	July 1, 2008
Last modified:	June 16, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)