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B2V4H5 (PNP_CLOBA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyribonucleotide nucleotidyltransferase

EC=2.7.7.8
Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase
Gene names
Name:pnp
Ordered Locus Names:CLH_1230
OrganismClostridium botulinum (strain Alaska E43 / Type E3) [Complete proteome] [HAMAP]
Taxonomic identifier508767 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length704 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP-Rule MF_01595

Catalytic activity

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_01595

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01595

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01595.

Sequence similarities

Belongs to the polyribonucleotide nucleotidyltransferase family.

Contains 1 KH domain.

Contains 1 S1 motif domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 704704Polyribonucleotide nucleotidyltransferase HAMAP-Rule MF_01595
PRO_1000192470

Regions

Domain552 – 61160KH
Domain621 – 68969S1 motif

Sites

Metal binding4851Magnesium By similarity
Metal binding4911Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
B2V4H5 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 4C3BD0CE43E9D131

FASTA70477,547
        10         20         30         40         50         60 
MNNVLSTNIA GKEMKVEFGK IGMLSNAATF MSYGDTVILT NVNASSEPRV GIDFFPLSVE 

        70         80         90        100        110        120 
YEERLYAVGK IPGGFIKREG RPSEKAILNG RAVDRTLRPL FPKGYRNDVQ VVCTVVSVEK 

       130        140        150        160        170        180 
DNLPEILAIN AASMALCLSS IPFAMPVAAV QVGLIDNNFI VNPNATEREE STLHLTVCAT 

       190        200        210        220        230        240 
KERVMMIEAG GNEIPEDIMI AAIKFGFDEC QNIINFQEEA VKKFGKEKDI PTLFTVDEEV 

       250        260        270        280        290        300 
EKDIKEFASD MIKEAMYITD KDERNAAIDA VNQKVKEEFG EKYEDKFGDI KEVLYNMQKK 

       310        320        330        340        350        360 
VVRHMLLKDK RRPDGRAFDQ VRPLGCEVGL LPRTHGTGLF TRGLTQVMTV ATLGAVGDIQ 

       370        380        390        400        410        420 
ILDGIDEAQS KRYMHHYNFP GYSVGEVKPL RGPGRREIGH GALAERALEP LIPSEEEFPY 

       430        440        450        460        470        480 
TIRLVSEVLS SNGSTSQASV CGSTLALLDA GVPLKRPAAG IAMGLITSED LSEEQVLTDI 

       490        500        510        520        530        540 
QGIEDFFGDM DFKVAGTTEG ITSIQVDTKL KGFSFNVVEN AIRDARKARM TILEKINECI 

       550        560        570        580        590        600 
SSPREDVSLY APKTETIQID PDKIRSVIGA GGKVINKIIQ DTGVKIDIKE DGSVFVSSSD 

       610        620        630        640        650        660 
HEGVKEAIKI IEGLTKDVKA GEIYLGKVTK ITTFGAFVEI LPNKEGLVHI SKLDKERVNK 

       670        680        690        700 
VEDVVSVGDE ILVKVTEIDS QGRINLSRKD VLLDQENKEN KEEK 

« Hide

References

[1]"Complete genome sequence of Clostridium botulinum E3 str. Alaska E43."
Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Alaska E43 / Type E3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001078 Genomic DNA. Translation: ACD52528.1.
RefSeqYP_001920624.1. NC_010723.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING508767.CLH_1230.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD52528; ACD52528; CLH_1230.
GeneID6317287.
KEGGcbt:CLH_1230.
PATRIC19418724. VBICloBot115804_1159.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1185.
HOGENOMHOG000218326.
KOK00962.
OMAPRWDWVA.
OrthoDBEOG6WT8CC.

Enzyme and pathway databases

BioCycCBOT508767:GHKO-1226-MONOMER.

Family and domain databases

Gene3D1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPMF_01595. PNPase.
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERPTHR11252. PTHR11252. 1 hit.
PfamPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFPIRSF005499. PNPase. 1 hit.
SMARTSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsTIGR03591. polynuc_phos. 1 hit.
PROSITEPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePNP_CLOBA
AccessionPrimary (citable) accession number: B2V4H5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families