B2UZH3 (ISPE_CLOBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	CLH_0463

Organism

Clostridium botulinum (strain Alaska E43 / Type E3) [Complete proteome] [HAMAP]

Taxonomic identifier

508767 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092076
Regions
	Nucleotide binding	91 – 101	11	ATP Potential
Sites
	Active site	8	1	By similarity
	Active site	133	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2UZH3 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 1BB24D62153F6504	FASTA	280	31,275
	10 20 30 40 50 60 MDIKAYAKIN ISLDVIGKRD DGYHLLKMIM QNIDLYDIVQ VEKIPNGIKL KCNKPYVPTD 70 80 90 100 110 120 ERNLAYKAAK LFKETYNIKS GIYINIEKNI PVSAGLAGGS TDAAAVLKIM NKMFNINVTQ 130 140 150 160 170 180 SELMNLGLKL GADVPYCICG GTALCEGIGE KVTKLKPFRD KILVVVKPPF GVSTKEVYKA 190 200 210 220 230 240 FDLSKVIFHP KTNELISNIE KNNIEFISNN MKNLLENVTL GRYKIISTIK EEINICGALG 250 260 270 280 SMMSGSGPTV FGFFDDMLKA QKCYEKMKEK YVDVFITRTI

References

[1]

"Complete genome sequence of Clostridium botulinum E3 str. Alaska E43." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Alaska E43 / Type E3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001078 Genomic DNA. Translation: ACD53283.1.
RefSeq	YP_001919871.1. NC_010723.1.
3D structure databases
ProteinModelPortal	B2UZH3.
ModBase	Search...
Protein-protein interaction databases
STRING	B2UZH3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6319065.
GenomeReviews	Gene locus CLH_0463 in contig CP001078_GR.
KEGG	cbt:CLH_0463.
PATRIC	19417196. VBICloBot115804_0405.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	SSTKEMY.
ProtClustDB	PRK00128.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_CLOBA

Accession

Primary (citable) accession number: B2UZH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program