Reviewed, UniProtKB/Swiss-Prot B2UZ31 (RNPH_CLOBA)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	CLH_0403

Organism

Clostridium botulinum (strain Alaska E43 / Type E3) [Complete proteome] [HAMAP]

Taxonomic identifier

508767 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity.
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Ribonuclease PH HAMAP MF_00564		PRO_1000129332

Sequences

	Sequence		Length	Mass (Da)
	B2UZ31-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 2F3D53D99B34EA38	FASTA	249	27,883
	10 20 30 40 50 60 MRIDGRKNEQ IRHVKVTRHY TKYAEGSVYI EVGDTKVLCN VSIEDKVPPF MKGTGSGWIT 70 80 90 100 110 120 AEYNMLPRST ETRKIRDIAR LKIDGRTMEI QRLIGRALRS VVDLKALGEK TLWIDCDVIQ 130 140 150 160 170 180 ADGGTRTTAI SGAFIALVDA VNKLHKIKPF KIYPIRSFVA AVSVGIVNEE KILDLCYQED 190 200 210 220 230 240 SKAKVDMNII MTDEGSFVEV QGTGEESPYT RTELNELLDL GEKGIKQMIN VQKESLKMDS LWIGTGGNN

References

[1]	"Complete genome sequence of Clostridium botulinum E3 str. Alaska E43." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001078 Genomic DNA. Translation: ACD51613.1.
RefSeq	YP_001919836.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6319100.
GenomeReviews	Gene locus CLH_0403 in contig CP001078_GR.
KEGG	cbt:CLH_0403.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YAMLPRA.
Family and domain databases
HAMAP	MF_00564. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR018336. Ribonuclease-PH_CS. IPR002381. RNase_PH_bac-type. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_CLOBA

Accession

Primary (citable) accession number: B2UZ31

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)