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B2UYI0 (ASSY_CLOBA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:CLH_2865
OrganismClostridium botulinum (strain Alaska E43 / Type E3) [Complete proteome] [HAMAP]
Taxonomic identifier508767 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 407407Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_1000089029

Regions

Nucleotide binding10 – 189ATP By similarity

Sites

Binding site881Citrulline By similarity
Binding site931Citrulline By similarity
Binding site1181ATP; via amide nitrogen By similarity
Binding site1201Aspartate By similarity
Binding site1241Aspartate By similarity
Binding site1241Citrulline By similarity
Binding site1251Aspartate By similarity
Binding site1281Citrulline By similarity
Binding site1771Citrulline By similarity
Binding site1861Citrulline By similarity
Binding site2631Citrulline By similarity
Binding site2751Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
B2UYI0 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: F1458A06A16E88DE

FASTA40745,720
        10         20         30         40         50         60 
MNKLNKVILA YSGGLDTSII IPWLKENYNC EVIAVCGNVG QKDELDGLEE KAIKTGASKL 

        70         80         90        100        110        120 
YMEDLTKEFV EDYIFPTIQA GAIYEGKYLL GTSFARPLIG KRLVEIAKVE GADAICHGCT 

       130        140        150        160        170        180 
GKGNDQVRFE LAVKAFDPDM KIIAPWRIWD IKSREDEITY AEARNVPIKI NHETNYSKDK 

       190        200        210        220        230        240 
NIWHLSHEGL DLEDPKNEPK YDEILELSNS LEKAPNEPTY ITLTFEKGNA VALNGEKMDA 

       250        260        270        280        290        300 
VTLLDELNKI GGKNAIGITD MVENRLVGMK SRGVYETPGG TILYKAHKDL EELCLDKETS 

       310        320        330        340        350        360 
HYKEQISLKF ADLVYNGLWF TPLREALSEF IKKTQETVTG EIKLKLYKGN IVNAGMTSPY 

       370        380        390        400 
SLYSEEYATF GEDAVYNQND SAGFITLYGL PTVVKAKMYQ SLKKEDK 

« Hide

References

[1]"Complete genome sequence of Clostridium botulinum E3 str. Alaska E43."
Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Alaska E43 / Type E3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001078 Genomic DNA. Translation: ACD53261.1.
RefSeqYP_001922242.1. NC_010723.1.

3D structure databases

ProteinModelPortalB2UYI0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING508767.CLH_2865.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD53261; ACD53261; CLH_2865.
GeneID6317637.
KEGGcbt:CLH_2865.
PATRIC19421975. VBICloBot115804_2777.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAIYNGYWW.
OrthoDBEOG6K9QCV.
ProtClustDBPRK00509.

Enzyme and pathway databases

BioCycCBOT508767:GHKO-2856-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_CLOBA
AccessionPrimary (citable) accession number: B2UYI0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: February 19, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways