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B2UY66 (THIM_CLOBA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:CLH_2856
OrganismClostridium botulinum (strain Alaska E43 / Type E3) [Complete proteome] [HAMAP]
Taxonomic identifier508767 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length278 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 278278Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000383841

Sites

Binding site481Substrate; via amide nitrogen By similarity
Binding site1241ATP By similarity
Binding site1751ATP By similarity
Binding site2021Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
B2UY66 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 7EF4D1FB435007A2

FASTA27829,634
        10         20         30         40         50         60 
MINEILDKVG RLLEEVRTKK PLVHSITNYI TATDCANVIL AVGGSPTMAD YVKEVEEIAS 

        70         80         90        100        110        120 
ISSAVVLNMG VISEKMVEAM ILAGKSANKN NVPVIFDPVG AGVANFRNKS AEKILSEVKV 

       130        140        150        160        170        180 
DIIRGNISEI KFICGLRSET KGVDASERDM NMGNDKKVIV AQELAKKLNC VVAITGVDDI 

       190        200        210        220        230        240 
ISDGKRNVIL SNGHKMLANV TGTGCMSSAL CGAFAGASDD YFIAAICAVL TMSISGEIAY 

       250        260        270 
EKSKGIGMGT FHISLIDAIS MMNENVIKEK ARVTTINR 

« Hide

References

[1]"Complete genome sequence of Clostridium botulinum E3 str. Alaska E43."
Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Alaska E43 / Type E3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001078 Genomic DNA. Translation: ACD51149.1.
RefSeqYP_001922233.1. NC_010723.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING508767.CLH_2856.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD51149; ACD51149; CLH_2856.
GeneID6319979.
KEGGcbt:CLH_2856.
PATRIC19421957. VBICloBot115804_2768.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMATISESTY.
OrthoDBEOG628F8M.

Enzyme and pathway databases

BioCycCBOT508767:GHKO-2847-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_CLOBA
AccessionPrimary (citable) accession number: B2UY66
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 1, 2008
Last modified: June 11, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways