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B2UY33 (DDL_CLOBA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:CLH_2823
OrganismClostridium botulinum (strain Alaska E43 / Type E3) [Complete proteome] [HAMAP]
Taxonomic identifier508767 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 301301D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000091173

Regions

Domain99 – 294196ATP-grasp
Nucleotide binding126 – 18156ATP By similarity

Sites

Metal binding2481Magnesium or manganese 1 By similarity
Metal binding2611Magnesium or manganese 1 By similarity
Metal binding2611Magnesium or manganese 2 By similarity
Metal binding2631Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B2UY33 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 77C73CA499819943

FASTA30133,208
        10         20         30         40         50         60 
MKVGVIMGGI SSEREISLKS GKSIVDSINK NKYEVVSIVI DEKEDIINKV KGIDFALLAL 

        70         80         90        100        110        120 
HGQFGEDGTV QSVLQTLGIP YSGCGPLSSS MCMDKDISKC ILKAANIRTA PWINLRKNDA 

       130        140        150        160        170        180 
INYEEIEKMG YPVVVKPTHG GSSVATFIIK EEKDIKDAVI EGFKWDSEVI IEKFIKGDEI 

       190        200        210        220        230        240 
TCPVFGDKML PVVAIKPKAE FFDFTAKYAD GGSDEFVTEL PKKLHEEVEK MALATYKALK 

       250        260        270        280        290        300 
CEVYSRVDMI VTEDKVPYIL EVNTLPGMTP NSLIPKSAAG VNISFPELID MIIDESMKVI 


R 

« Hide

References

[1]"Complete genome sequence of Clostridium botulinum E3 str. Alaska E43."
Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Alaska E43 / Type E3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001078 Genomic DNA. Translation: ACD53784.1.
RefSeqYP_001922200.1. NC_010723.1.

3D structure databases

ProteinModelPortalB2UY33.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING508767.CLH_2823.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD53784; ACD53784; CLH_2823.
GeneID6317428.
KEGGcbt:CLH_2823.
PATRIC19421893. VBICloBot115804_2736.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAKYTEGFA.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycCBOT508767:GHKO-2814-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_CLOBA
AccessionPrimary (citable) accession number: B2UY33
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: February 19, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways