Reviewed, UniProtKB/Swiss-Prot B2UXU5 (FTHS_CLOBA)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase
      Short name=FHS
      Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	CLH_0166

Organism

Clostridium botulinum (strain Alaska E43 / Type E3) [Complete proteome] [HAMAP]

Taxonomic identifier

508767 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_1000146676
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2UXU5-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 89DA173F6453EE52	FASTA	556	60,263
	10 20 30 40 50 60 MKTDIEIAQE AEMLHIRNIA EKLGLDEEDI EYYGKYKCKI SLDVYNKVKN NRDGKLVLVT 70 80 90 100 110 120 AINPTPAGEG KSTVTVGLGD ALNKMGKNTV IALREPSLGP VFGIKGGAAG GGYAQVVPME 130 140 150 160 170 180 DINLHFTGDM HAITSANNLL SAAIDNHIHQ GNNLRIDSRR IIFKRVMDMN DRALRKIIVG 190 200 210 220 230 240 MGGKINGFVR EDGFTITVAS EIMAILCLAS DLEDLKHRMG DILIAYDLDG NPVYAKQLEI 250 260 270 280 290 300 QGAMALLMKD AIKPNLVQTL ENTPALIHGG PFANIAHGCN SIMATKLSLK LGDIVVTEAG 310 320 330 340 350 360 FGADLGAEKF FDIKCRYGNL KPCCVVIVAT IRALKHHGGV AKADLNIPNV EALRLGIANL 370 380 390 400 410 420 EKQIENIKKF NVEPVVAINK FVSDSDEEVE FIKEFCEKLG VKVALSDVWA KGGDGGIELG 430 440 450 460 470 480 EAVLDVIEKD KSSFKTLYKA DNTIEEKILT IAKEIYGADG VVYSNEAKKQ IGELVKFNLD 490 500 510 520 530 540 KLPICMAKTQ YSLSDNPNLL AKPSGFNINV QEIRVSNGAG FIVVQTGNIM TMPGLPKVPA 550 ANKMDVLKDG EIVGLF

References

[1]	"Complete genome sequence of Clostridium botulinum E3 str. Alaska E43." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001078 Genomic DNA. Translation: ACD51878.1.
RefSeq	YP_001919613.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6318973.
GenomeReviews	Gene locus CLH_0166 in contig CP001078_GR.
KEGG	cbt:CLH_0166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GMKGGAT.
Family and domain databases
HAMAP	MF_01543. [Tree]
InterPro	IPR000559. For_THF_ligase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_CLOBA

Accession

Primary (citable) accession number: B2UXU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)