Reviewed, UniProtKB/Swiss-Prot B2UXS0 (SYN_CLOBA)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	CLH_0134

Organism

Clostridium botulinum (strain Alaska E43 / Type E3) [Complete proteome] [HAMAP]

Taxonomic identifier

508767 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000128204

Sequences

	Sequence		Length	Mass (Da)
	B2UXS0-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: F63ADDFD06982A1A	FASTA	464	53,341
	10 20 30 40 50 60 MKNTVLIKKI YRETDQFLSK EVMISGWIRT LRASNAFGFI EINDGSFFKN IQVVFDDKLG 70 80 90 100 110 120 NFKEISKLPI SSSISVVGTL VATPDAKQPF EIQAKEIVIE GMSNSDYPLQ KKRHTFEYLR 130 140 150 160 170 180 SIAHLRPRSN AFSATFRVRS VAAFAIHKFF QEQGFVYTHT PIITGSDCEG AGEMFRVTTL 190 200 210 220 230 240 DPKAPELTKE GNIDYTKDFF GKETNLTVSG QLNAECFALA FRNIYTFGPT FRAENSNTTR 250 260 270 280 290 300 HAAEFWMIEP EIAFADLQDD MELAEAMLKY VIKYVMDECP EELQFFNSFV DKGLLERLNH 310 320 330 340 350 360 VVSSDFAKVT YTEAVEILEK CDKEFDYDVS WGIDLQTEHE RYLTEEHFKK PLFVTDYPKE 370 380 390 400 410 420 IKAFYMRMNE DNKTVAATDL LVPGIGEIIG GSQREERLDV LEARMAELGL KKEDYWWYLE 430 440 450 460 LRKYGETKHA GFGLGFERLI MYITGMTNIR DVIPFPRTPG TSEF

References

[1]	"Complete genome sequence of Clostridium botulinum E3 str. Alaska E43." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001078 Genomic DNA. Translation: ACD51460.1.
RefSeq	YP_001919588.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6320454.
GenomeReviews	Gene locus CLH_0134 in contig CP001078_GR.
KEGG	cbt:CLH_0134.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LQKKRHS.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_CLOBA

Accession

Primary (citable) accession number: B2UXS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)