Skip Header

Contribute Send feedback
Read comments (?) or add your own

B2UXA9 (B2UXA9_CLOBA) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP MF_02003
EC=6.1.1.5 HAMAP MF_02003
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP MF_02003
Gene names
Name:ileS HAMAP MF_02003 EMBL ACD53106.1
Ordered Locus Names:CLH_0078
OrganismClostridium botulinum (strain Alaska E43 / Type E3) [Complete proteome] [HAMAP]
Taxonomic identifier508767 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length1036 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003

Cofactor

Zinc By similarity. HAMAP MF_02003

Subunit structure

Monomer By similarity. HAMAP MF_02003

Subcellular location

Cytoplasm By similarity HAMAP MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. HAMAP MF_02003

Sequences

Sequence LengthMass (Da)Tools
B2UXA9 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: DEF83B059C8B33CD

FASTA1,036118,938
        10         20         30         40         50         60 
MYKKVDSSKG FVNMEHDVAT LWNEKDIIKK SFDSNQDGEY FTFYDGPPTA NGKPHVGHIL 

        70         80         90        100        110        120 
TRVMKDIIPR YKVMKGYKVI RKAGWDTHGL PVELEIEKKL GISGKEQIEE YGVEKFVKEC 

       130        140        150        160        170        180 
KESVFTYVSM WEKMTEQIGY WVDMEHPYVT YHNPYIESVW WALKQMWDKE LLYEGHKVMP 

       190        200        210        220        230        240 
YCPRCGTALS SHEVAQGYKD VKDLTAIAKF KVQGEENKFI LAWTTTPWTL PSNLALCINK 

       250        260        270        280        290        300 
AYTYIEAKVE EEVYVLAKDL ADKVLGEDYE IIREFKGTEL LGTKYEQLMP FGKVEGKAFE 

       310        320        330        340        350        360 
VIHGDYVTLT DGTGIVHIAP AYGEDDSLVA KANGIAFINL VDKEGKFVEE VTPWAGKFVK 

       370        380        390        400        410        420 
KCDDSICKWL EENNKLFKTA RHLHSYPHCW RCDTPLLYYP KESWFVRMTS LRDKLLENNN 

       430        440        450        460        470        480 
KINWCPDNIR TGRFGKFLEN VIDWGISRDR YWGTPLPIWE CECGHKECIG SIAELKEKGI 

       490        500        510        520        530        540 
NVPEDIELHK PYIDNVHLKC AHCGKEMKRT KEVIDCWFDS GSMPFAQLHY PFENKELFEQ 

       550        560        570        580        590        600 
NYPAQFISEA VDQTRGWFYT LLAISTAVFD RNPFENCIVL GHVLDKKGLK MSKSKGNVVD 

       610        620        630        640        650        660 
PFEVLSSQGA DATRWHFYTA SAPWLPTRFS TDDVGESQRK FLSTLWNVYS FYVLYAEIDS 

       670        680        690        700        710        720 
FNPLEYADFK SENVMDKWIM SKLNTLIKTV DEKLNSYDIT AAALSIEEFT DELSNWYVRR 

       730        740        750        760        770        780 
NRSRYWTETI NDDKIGAYVT LYRVLVTLSK VAAPFVPFIT EEIYQNLVVN LDNAAEESIH 

       790        800        810        820        830        840 
LTSWPQVDES AINKELEKEM DLAYTIVKLG RSARNGANIK NRQPLSEILV SVASLPEYYG 

       850        860        870        880        890        900 
DIITNELNIK AIKFGADLSE HVNFELKPNL PVLGRAYGKL IPGIRKEIAA RNQMELAQKI 

       910        920        930        940        950        960 
KGGDVEVINV DGTEIELNSE SILVTMQGLE GYAFAGEGEI GVVLDTHITN ELREEGHVRE 

       970        980        990       1000       1010       1020 
VISKIQNMRK DKGFEVADKI KLYVANNELL LEIIKKFSDT IKKETLTSEI IYNENCDYTQ 

      1030 
TIINGENLDM TVEVVK

« Hide

References

[1]"Complete genome sequence of Clostridium botulinum E3 str. Alaska E43."
Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001078 Genomic DNA. Translation: ACD53106.1.
RefSeqYP_001919532.1. NC_010723.1.

3D structure databases

ProteinModelPortalB2UXA9.
ModBaseSearch...

Protein-protein interaction databases

STRINGB2UXA9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6318276.
GenomeReviewsGene locus CLH_0078 in contig CP001078_GR.
KEGGcbt:CLH_0078.
PATRIC19416434. VBICloBot115804_0066.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG577712.
OMAPYITYDN.
ProtClustDBPRK06039.

Family and domain databases

HAMAPMF_02003. Ile_tRNA_synth_type2.
[Tree]
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-synt.
IPR023586. Ile-tRNA-synt_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
Gene3DG3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01870.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 2 hits.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. IleS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB2UXA9_CLOBA
AccessionPrimary (citable) accession number: B2UXA9
Entry history
Integrated into UniProtKB/TrEMBL: July 1, 2008
Last sequence update: July 1, 2008
Last modified: January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info