Reviewed, UniProtKB/Swiss-Prot B2UWX8 (LUXS_CLOBA)

Names and origin

Protein names

Recommended name:
    S-ribosylhomocysteine lyase
    EC=4.4.1.21
Alternative name(s):
    Autoinducer-2 production protein luxS
    AI-2 synthesis protein

Gene names

Name:	luxS
Ordered Locus Names:	CLH_2583

Organism

Clostridium botulinum (strain Alaska E43 / Type E3) [Complete proteome] [HAMAP]

Taxonomic identifier

508767 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity. HAMAP MF_00091
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091
Cofactor	Binds 1 iron ion per subunit By similarity. HAMAP MF_00091
Subunit structure	Homodimer By similarity. HAMAP MF_00091
Sequence similarities	Belongs to the luxS family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Quorum sensing
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	quorum sensing Inferred from electronic annotation. Source: HAMAP
Molecular function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 151	151	S-ribosylhomocysteine lyase HAMAP MF_00091		PRO_1000093303
Sites
	Metal binding	54	1	Iron By similarity
	Metal binding	58	1	Iron By similarity
	Metal binding	121	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2UWX8-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 4B70CF7199957CD1	FASTA	151	17,164
	10 20 30 40 50 60 MEKVESFELD HRKVKAPYIR KCCLLDGKCG DKVTKFDIRF LQPNKEEFGT AAMHGLEHLL 70 80 90 100 110 120 AHELRAKLEG IIDLSPMGCR TGFYLSIWGD REASEIKEAL EYSLEKVLEA KEIPAANDIQ 130 140 150 CGNYRDLSLF GAKEYAKEAL ERGFSLNIYG E

References

[1]	"Complete genome sequence of Clostridium botulinum E3 str. Alaska E43." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001078 Genomic DNA. Translation: ACD51959.1.
RefSeq	YP_001921963.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6320248.
GenomeReviews	Gene locus CLH_2583 in contig CP001078_GR.
KEGG	cbt:CLH_2583.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG347473.
OMA	YFGPMGC.
Family and domain databases
HAMAP	MF_00091. LuxS. [Tree]
InterPro	IPR011249. Metalloenz_metal-bd. IPR003815. S-ribosylhomocysteinase. [Graphical view]
Gene3D	G3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. S-ribosylhomocysteinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

LUXS_CLOBA

Accession

Primary (citable) accession number: B2UWX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	January 19, 2010
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)