B2UW72 (LSPA_HELPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	HPSH_00360

Organism

Helicobacter pylori (strain Shi470) [Complete proteome] [HAMAP]

Taxonomic identifier

512562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP-Rule MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP-Rule MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP-Rule MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	Lipoprotein signal peptidase HAMAP-Rule MF_00161		PRO_1000097259
Regions
	Transmembrane	10 – 30	21	Helical; Potential
	Transmembrane	58 – 78	21	Helical; Potential
	Transmembrane	84 – 104	21	Helical; Potential
	Transmembrane	122 – 142	21	Helical; Potential
Sites
	Active site	105	1	By similarity
	Active site	131	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2UW72 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: CEB534159D5C0E88	FASTA	157	17,906
	10 20 30 40 50 60 MLKTTQKSLL IFIVVFSLIF GTDQAIKYAI LEGFRYESLI IDIVLVFNKG VAFSLLSFLE 70 80 90 100 110 120 GGLKYLQILL ILGLFIFLMC QKELFKNHAI EFGMVFGAGV SNVLDRFVHG GVVDYVYYHY 130 140 150 GFDFAIFNFA DVMIDVGVGV LLLRQFFFKQ KQNKIKA

References

[1]	"Genome sequence of Helicobacter pylori from the remote Amazon: traces of Asian ancestry of the first Americans." Kersulyte D., Kalia A., Gilman R.H., Berg D.E. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Shi470.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001072 Genomic DNA. Translation: ACD47537.1.
RefSeq	YP_001909567.1. NC_010698.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	512562.HPSH_00360.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD47537; ACD47537; HPSH_00360.
GeneID	6296079.
KEGG	hps:HPSH_00360.
PATRIC	20611501. VBIHelPyl23559_0069.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0597.
HOGENOM	HOG000096992.
KO	K03101.
OMA	DFAVFNF.
ProtClustDB	PRK00376.
Enzyme and pathway databases
BioCyc	HPYL512562:GHHZ-74-MONOMER.
UniPathway	UPA00665.
Family and domain databases
HAMAP	MF_00161. LspA.
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_HELPS

Accession

Primary (citable) accession number: B2UW72

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program