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B2UVG2

- SYI_HELPS

UniProt

B2UVG2 - SYI_HELPS

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Helicobacter pylori (strain Shi470)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
Binding sitei613 – 6131ATPUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation
Metal bindingi913 – 9131ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHPYL512562:GHHZ-1447-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:HPSH_07255
OrganismiHelicobacter pylori (strain Shi470)
Taxonomic identifieri512562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000008831: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 920920Isoleucine--tRNA ligasePRO_1000189172Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi512562.HPSH_07255.

Structurei

3D structure databases

ProteinModelPortaliB2UVG2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi610 – 6145"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2UVG2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEYKDTLNL NATTFSMKGN LSVNEPKTYA KWQEQQAFKR MQARKDNHGD
60 70 80 90 100
FTLHDGPPYA NGHLHLGHAL NKILKDIVVK REYFKGKKIY YTPGWDCHGL
110 120 130 140 150
PIEQQILEQL EKEKTSLENP TLFREKCRDH AKKFLEIQKN EFLQLGVLGD
160 170 180 190 200
FDDPYKTMDF KFEASIYRAL VEVAKKGLLK ERHKPIYWSY ACESALAEAE
210 220 230 240 250
VEYKMKKSPS IFVAFGLKKE SLEKLKVKKA SLVIWTTTPW TLYANVAIAL
260 270 280 290 300
KKDAIYALTQ KGYLVAKALH EKLAALGVVD SEIVHEFNAN DLEYLKALNP
310 320 330 340 350
LNQRNSLITL GEHVGLEDGT GAVHTAPGHG EEDYYLGLKY NLEVLMSVDE
360 370 380 390 400
KGCYDEGIIH NQLLDESYLG EHVFKAQKRI IEQLGDSLLL EQEIEHSYPH
410 420 430 440 450
CWRTHKPVIY RATTQWFILM DEPFIQNDGS QKTLREVALD AIEKVEFVPN
460 470 480 490 500
SGKNRLKIMI ENRPDWCLSR QRKWGVPLAF FIDKRTNKPC FESEVLEHTA
510 520 530 540 550
KLFEERGCDV WWEYSVKDLL PPNYQDNATY YEKVMHILDV WFDSGSTFKA
560 570 580 590 600
VLEDYQGEKG QSPSDVVLEG SDQHRGWFQS SLLIGCILNN QAPFKKVITH
610 620 630 640 650
GFIVDEKGEK MSKSKGNVVS LDKLLKTHGS DVVRLWVAFN DYQNDLRVSQ
660 670 680 690 700
TFFTQTEQHY KKFRNTLKFL LANFSDMDLK NLERSHNFSP LDHFILESLE
710 720 730 740 750
TISAGVNSAF EEHDFVKGLN VLMAFVTNEL SGIYLDACKD SLYCDSKNNE
760 770 780 790 800
KRQAIQMVLL ATASKLCYFL APILTHTIEE VLAHSQVLRI FLQAKDVFDL
810 820 830 840 850
KDISVSEKLH LKEFKKPENF EAVLALRSAF NEELDRLKKE GVIKNSLECA
860 870 880 890 900
IEVGEKALCE NLVEELLMVS FVGIAREKLS ETPAFTLFKA PFYKCPRCWR
910 920
FKSELENAPC KRCEQVLKER
Length:920
Mass (Da):105,931
Last modified:July 1, 2008 - v1
Checksum:iCFC2351D9968138A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001072 Genomic DNA. Translation: ACD48844.1.
RefSeqiYP_001910874.1. NC_010698.2.

Genome annotation databases

EnsemblBacteriaiACD48844; ACD48844; HPSH_07255.
GeneIDi6296355.
KEGGihps:HPSH_07255.
PATRICi20614247. VBIHelPyl23559_1411.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001072 Genomic DNA. Translation: ACD48844.1 .
RefSeqi YP_001910874.1. NC_010698.2.

3D structure databases

ProteinModelPortali B2UVG2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 512562.HPSH_07255.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD48844 ; ACD48844 ; HPSH_07255 .
GeneIDi 6296355.
KEGGi hps:HPSH_07255.
PATRICi 20614247. VBIHelPyl23559_1411.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HPYL512562:GHHZ-1447-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Helicobacter pylori from the remote Amazon: traces of Asian ancestry of the first Americans."
    Kersulyte D., Kalia A., Gilman R.H., Berg D.E.
    Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Shi470.

Entry informationi

Entry nameiSYI_HELPS
AccessioniPrimary (citable) accession number: B2UVG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 1, 2008
Last modified: November 26, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3