B2UVG2 (SYI_HELPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	HPSH_07255

Organism

Helicobacter pylori (strain Shi470) [Complete proteome] [HAMAP]

Taxonomic identifier

512562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	920 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 920	920	Isoleucine--tRNA ligase HAMAP-Rule MF_02002		PRO_1000189172
Regions
	Motif	58 – 68	11	"HIGH" region HAMAP-Rule MF_02002
	Motif	610 – 614	5	"KMSKS" region HAMAP-Rule MF_02002
Sites
	Metal binding	895	1	Zinc By similarity
	Metal binding	898	1	Zinc By similarity
	Metal binding	910	1	Zinc By similarity
	Metal binding	913	1	Zinc By similarity
	Binding site	569	1	Aminoacyl-adenylate By similarity
	Binding site	613	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2UVG2 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: CFC2351D9968138A	FASTA	920	105,931
	10 20 30 40 50 60 MKEYKDTLNL NATTFSMKGN LSVNEPKTYA KWQEQQAFKR MQARKDNHGD FTLHDGPPYA 70 80 90 100 110 120 NGHLHLGHAL NKILKDIVVK REYFKGKKIY YTPGWDCHGL PIEQQILEQL EKEKTSLENP 130 140 150 160 170 180 TLFREKCRDH AKKFLEIQKN EFLQLGVLGD FDDPYKTMDF KFEASIYRAL VEVAKKGLLK 190 200 210 220 230 240 ERHKPIYWSY ACESALAEAE VEYKMKKSPS IFVAFGLKKE SLEKLKVKKA SLVIWTTTPW 250 260 270 280 290 300 TLYANVAIAL KKDAIYALTQ KGYLVAKALH EKLAALGVVD SEIVHEFNAN DLEYLKALNP 310 320 330 340 350 360 LNQRNSLITL GEHVGLEDGT GAVHTAPGHG EEDYYLGLKY NLEVLMSVDE KGCYDEGIIH 370 380 390 400 410 420 NQLLDESYLG EHVFKAQKRI IEQLGDSLLL EQEIEHSYPH CWRTHKPVIY RATTQWFILM 430 440 450 460 470 480 DEPFIQNDGS QKTLREVALD AIEKVEFVPN SGKNRLKIMI ENRPDWCLSR QRKWGVPLAF 490 500 510 520 530 540 FIDKRTNKPC FESEVLEHTA KLFEERGCDV WWEYSVKDLL PPNYQDNATY YEKVMHILDV 550 560 570 580 590 600 WFDSGSTFKA VLEDYQGEKG QSPSDVVLEG SDQHRGWFQS SLLIGCILNN QAPFKKVITH 610 620 630 640 650 660 GFIVDEKGEK MSKSKGNVVS LDKLLKTHGS DVVRLWVAFN DYQNDLRVSQ TFFTQTEQHY 670 680 690 700 710 720 KKFRNTLKFL LANFSDMDLK NLERSHNFSP LDHFILESLE TISAGVNSAF EEHDFVKGLN 730 740 750 760 770 780 VLMAFVTNEL SGIYLDACKD SLYCDSKNNE KRQAIQMVLL ATASKLCYFL APILTHTIEE 790 800 810 820 830 840 VLAHSQVLRI FLQAKDVFDL KDISVSEKLH LKEFKKPENF EAVLALRSAF NEELDRLKKE 850 860 870 880 890 900 GVIKNSLECA IEVGEKALCE NLVEELLMVS FVGIAREKLS ETPAFTLFKA PFYKCPRCWR 910 920 FKSELENAPC KRCEQVLKER

References

[1]	"Genome sequence of Helicobacter pylori from the remote Amazon: traces of Asian ancestry of the first Americans." Kersulyte D., Kalia A., Gilman R.H., Berg D.E. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Shi470.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001072 Genomic DNA. Translation: ACD48844.1.
RefSeq	YP_001910874.1. NC_010698.2.
3D structure databases
ProteinModelPortal	B2UVG2.
ModBase	Search...
Protein-protein interaction databases
STRING	512562.HPSH_07255.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD48844; ACD48844; HPSH_07255.
GeneID	6296355.
KEGG	hps:HPSH_07255.
PATRIC	20614247. VBIHelPyl23559_1411.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HOG000246402.
KO	K01870.
OMA	KQVLTHG.
ProtClustDB	PRK05743.
Enzyme and pathway databases
BioCyc	HPYL512562:GHHZ-1415-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_02002. Ile_tRNA_synth_type1.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023585. Ile-tRNA-ligase_type1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_HELPS

Accession

Primary (citable) accession number: B2UVG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program