Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Helicobacter pylori (strain Shi470)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi484MagnesiumUniRule annotation1
Metal bindingi490MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:HPSH_06280
OrganismiHelicobacter pylori (strain Shi470)
Taxonomic identifieri512562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003819031 – 688Polyribonucleotide nucleotidyltransferaseAdd BLAST688

Proteomic databases

PRIDEiB2UUX6.

Structurei

3D structure databases

ProteinModelPortaliB2UUX6.
SMRiB2UUX6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini550 – 609KHUniRule annotationAdd BLAST60
Domaini626 – 688S1 motifUniRule annotationAdd BLAST63

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218326.
KOiK00962.
OMAiRYMHNYN.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

B2UUX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFITITSSN KTEEFALKQV AKQATSSLMY RLGKTIILAS VCVEREPVGE
60 70 80 90 100
DFLPLVVQFL EKSYAAGKIP GGFVKREGRA QDFEILTSRL IDRTLRPLFP
110 120 130 140 150
KDYRYPTQIT LMVLSHDVEN DLQVSALNAA SAALFLSHIA PIKSVSACRI
160 170 180 190 200
ARIGNEFIIN PNTSLLNQSS LDLFVSGTKE SLNMIEMRSL GQQLNALEEP
210 220 230 240 250
LMLKALELAQ KSLKETCTLY EEIFTPHQNE LLFKESQGII FNERLLDLLK
260 270 280 290 300
NQYFDEIIKG IESSALSERE NVFNEIARKI SEAHSEFSLK EIELSLEKVK
310 320 330 340 350
KTEIRCMIIK DKIRPDKRAL EEVRPILIES DLLPMAHSSI LFTRGQTQSL
360 370 380 390 400
VVGVLGTDND AQTHESLEHK APIKERFMFH YNFPPFCVGE ASSIGATSRR
410 420 430 440 450
ELGHGNLAKR ALETSIKNKD QVIRLVSEIL ESNGSSSMAS VCAGSLALYA
460 470 480 490 500
SGVEIYDLIA GVAMGMVSEG QDHAILSDIS GLEDAEGDMD FKIAGNLEGI
510 520 530 540 550
TAMQMDTKMS GIQLEVLYQA LLQAKKAREH ILKIMHEAKE KIVINFSHLP
560 570 580 590 600
TTEIFNVAPD KIVEIIGQGG RVIREIVEKF EVKIDLNKPS GEVKIMGNKE
610 620 630 640 650
RVLKTKEFIL NYLHSLDQEL EQYAIDEVLE AQVKRIVDFG AFLSLPKGGE
660 670 680
GLLRKQNMDR CQVVLKEGDS IKCRVISFNK GKIALDLA
Length:688
Mass (Da):76,821
Last modified:July 1, 2008 - v1
Checksum:iFC36156FD87EEEFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001072 Genomic DNA. Translation: ACD48658.1.
RefSeqiWP_000345886.1. NC_010698.2.

Genome annotation databases

EnsemblBacteriaiACD48658; ACD48658; HPSH_06280.
KEGGihps:HPSH_06280.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPNP_HELPS
AccessioniPrimary (citable) accession number: B2UUX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: July 1, 2008
Last modified: June 7, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families