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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Helicobacter pylori (strain Shi470)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi258Manganese 2UniRule annotation1
Metal bindingi263Manganese 1UniRule annotation1
Metal bindingi263Manganese 2UniRule annotation1
Active sitei270UniRule annotation1
Metal bindingi281Manganese 2UniRule annotation1
Metal bindingi340Manganese 1UniRule annotation1
Metal bindingi342Manganese 1UniRule annotation1
Metal bindingi342Manganese 2UniRule annotation1
Active sitei344UniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation (EC:3.4.11.1UniRule annotation)
Alternative name(s):
Leucine aminopeptidaseUniRule annotation (EC:3.4.11.10UniRule annotation)
Short name:
LAPUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Ordered Locus Names:HPSH_04025
OrganismiHelicobacter pylori (strain Shi470)
Taxonomic identifieri512562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000983261 – 496Probable cytosol aminopeptidaseAdd BLAST496

Structurei

3D structure databases

ProteinModelPortaliB2UTQ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000243129.
KOiK01255.
OMAiNTPPMIG.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2UTQ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKIKLEKTT FENAKAECGL VFIINKDFDH AWVKNKKLLE TFKYEGEGVF
60 70 80 90 100
LDQENKILYA GVKEDDVHLL RESACLAVRT LKKLAFKSVK VGVYTCDTHS
110 120 130 140 150
KDNALLENLK ALFLGLKLGL YEYDTFKSNK KESVLKEAIV ALELHKPCEK
160 170 180 190 200
TCANSLEKSA KEALKYAEIM TESLNIVRDL VNTPPMIATP VYMAEVAQKV
210 220 230 240 250
AKENHLEIHV HDEKFLEEKK MNAFLAVNKA SLGVNPPRLI HLVYKPKKAK
260 270 280 290 300
KKIALVGKGL TYDCGGLSLK PADYMVTMKA DKGGGSAVIG LLNALAKLGV
310 320 330 340 350
EAEVHGIIGA TENMIGPAAY KPDDILISKE GKSIEVRNTD AEGRLVLADC
360 370 380 390 400
LSYAQDLSPD VIVDFATLTG ACVVGLGEFT SAIMGHNEEL KNLFETSGLE
410 420 430 440 450
SGELLAKLPF NRHLKKLIES KIADVCNISS SRYGGAITAG LFLNEFIRDE
460 470 480 490
FKDKWLHIDI AGPAYVEKEW DVNSFGASGA GVRACTAFVE EFLKKA
Length:496
Mass (Da):54,537
Last modified:July 1, 2008 - v1
Checksum:iA70A431A569912CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001072 Genomic DNA. Translation: ACD48240.1.
RefSeqiWP_000912817.1. NC_010698.2.

Genome annotation databases

EnsemblBacteriaiACD48240; ACD48240; HPSH_04025.
KEGGihps:HPSH_04025.
PATRICi20612965. VBIHelPyl23559_0794.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001072 Genomic DNA. Translation: ACD48240.1.
RefSeqiWP_000912817.1. NC_010698.2.

3D structure databases

ProteinModelPortaliB2UTQ8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD48240; ACD48240; HPSH_04025.
KEGGihps:HPSH_04025.
PATRICi20612965. VBIHelPyl23559_0794.

Phylogenomic databases

HOGENOMiHOG000243129.
KOiK01255.
OMAiNTPPMIG.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPA_HELPS
AccessioniPrimary (citable) accession number: B2UTQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: November 2, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.