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B2UT94 (DDL_HELPS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:HPSH_03150
OrganismHelicobacter pylori (strain Shi470) [Complete proteome] [HAMAP]
Taxonomic identifier512562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 347347D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000091186

Regions

Domain134 – 332199ATP-grasp
Nucleotide binding161 – 21656ATP By similarity

Sites

Metal binding2881Magnesium or manganese 1 By similarity
Metal binding3001Magnesium or manganese 1 By similarity
Metal binding3001Magnesium or manganese 2 By similarity
Metal binding3021Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B2UT94 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 386DE0C341B317A9

FASTA34739,458
        10         20         30         40         50         60 
MEFCVLFGGA SFEHEISIVS AIALKEALKG RIKYFIFLDE NHHFYLIEES NMHSKYFAQI 

        70         80         90        100        110        120 
KEKKLPPLIL TCNGLLKNSF LGAKIIELPL VINLVHGGDG EDGKLASLLE FYRIAFIGPG 

       130        140        150        160        170        180 
IEASVLSYNK YLTKLYAKDL GVKALDHVLL NEKNRANALN LIGFNFPFII KPSNAGSSLG 

       190        200        210        220        230        240 
VSVVKEEKEL IYALDGAFEY SKEILIEPFI QGVKEYNLAG CKIKKDFCFS YVEEPNKQEF 

       250        260        270        280        290        300 
LDFKQKYLDF SRNKAPKANL SNALEEQLKE NFKKLYNDLF DGALIRCDFF VIENEVYLNE 

       310        320        330        340 
INPIPGSLAN YLFDDFKTTL ENLAQSLPKT PKIQIKNSYL LQIQKNK 

« Hide

References

[1]"Genome sequence of Helicobacter pylori from the remote Amazon: traces of Asian ancestry of the first Americans."
Kersulyte D., Kalia A., Gilman R.H., Berg D.E.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Shi470.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001072 Genomic DNA. Translation: ACD48076.1.
RefSeqYP_001910106.1. NC_010698.2.

3D structure databases

ProteinModelPortalB2UT94.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING512562.HPSH_03150.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD48076; ACD48076; HPSH_03150.
GeneID6296480.
KEGGhps:HPSH_03150.
PATRIC20612603. VBIHelPyl23559_0613.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000102494.
KOK01921.
OMAHNGLLKN.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycHPYL512562:GHHZ-631-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_HELPS
AccessionPrimary (citable) accession number: B2UT94
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways