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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Helicobacter pylori (strain Shi470)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37Substrate; via amide nitrogenUniRule annotation1
Binding sitei113ATPUniRule annotation1
Binding sitei158ATPUniRule annotation1
Binding sitei185Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:HPSH_02575
OrganismiHelicobacter pylori (strain Shi470)
Taxonomic identifieri512562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003838661 – 259Hydroxyethylthiazole kinaseAdd BLAST259

Structurei

3D structure databases

ProteinModelPortaliB2USY3.
SMRiB2USY3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000114352.
KOiK00878.
OMAiKRPLVHN.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiView protein in InterPro
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
PANTHERiPTHR44423. PTHR44423. 1 hit.
PfamiView protein in Pfam
PF02110. HK. 1 hit.
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

B2USY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKELRQKRP LVHNITNYVV AQFVANGLLA LGASPLMSDA IAEMQDLAKI
60 70 80 90 100
SDALAINIGT LNERTILCAK EAIKHYKALN KPIVLDPVGC SASALRHGTS
110 120 130 140 150
LELLESEGIS VLRGNAAELG SLVGISCGSK GLDSHYATTP IEIVKLVAQK
160 170 180 190 200
YSVIAVMTGK TDYVSDGKKV FSITGGSEYL ALITGAGCLH AAACASFLSL
210 220 230 240 250
RKDPLDSMAQ LCALYKQAAF NAQKKVSENN GSNGSFLFYF LDALSLPIKL

ENSLIKEEL
Length:259
Mass (Da):27,577
Last modified:July 1, 2008 - v1
Checksum:i4DED706A8A242511
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001072 Genomic DNA. Translation: ACD47965.1.
RefSeqiWP_000910638.1. NC_010698.2.

Genome annotation databases

EnsemblBacteriaiACD47965; ACD47965; HPSH_02575.
KEGGihps:HPSH_02575.

Similar proteinsi

Entry informationi

Entry nameiTHIM_HELPS
AccessioniPrimary (citable) accession number: B2USY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 1, 2008
Last modified: November 22, 2017
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families