Reviewed, UniProtKB/Swiss-Prot B2USX4 (GUAC_HELPS)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	HPSH_02530

Organism

Helicobacter pylori (strain Shi470) [Complete proteome] [HAMAP]

Taxonomic identifier

512562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	GMP reductase HAMAP MF_01511		PRO_1000146137
Regions
	Nucleotide binding	203 – 226	24	NADP Potential
Sites
	Active site	174	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2USX4-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: EE7A0B8B335EB64C	FASTA	325	35,972
	10 20 30 40 50 60 MKIFDYENIQ LIPNKCIVNS RSECDTTVTL GRHTFKMPIV PANMQTIIND SIAEFLAENG 70 80 90 100 110 120 YFYIMHRFDG AARIPFVKKM KERQWISSIS VGVKKEEYLF IEELAKQKLA SDYITIDIAH 130 140 150 160 170 180 GHSNSVIEMI QHIKTHLPET FVIAGNVGTP EAVRELENAG ADATKVGIGP GKACITKIKT 190 200 210 220 230 240 GFGTGGWQLA ALRWCAKAAR KPIIADGGIR THGDIAKSIR FGATMVMIGS LFAGHEESSG 250 260 270 280 290 300 ETKIENGIAY KEYFGSASEF QKGEKKNIEG KKIWIQHKGS LKDTLIEMHQ DLQSSISYAG 310 320 GRDLEAIRKV DYVIVKNSIF NGDAI

References

[1]	"Genome sequence of Helicobacter pylori from the remote Amazon: traces of Asian ancestry of the first Americans." Kersulyte D., Kalia A., Gilman R.H., Berg D.E. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001072 Genomic DNA. Translation: ACD47956.1.
RefSeq	YP_001909986.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6296520.
GenomeReviews	Gene locus HPSH_02530 in contig CP001072_GR.
KEGG	hps:HPSH_02530.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298985.
OMA	FAGHEES.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_HELPS

Accession

Primary (citable) accession number: B2USX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	February 9, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)