B2USR1 (ISPDF_HELPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional enzyme IspD/IspF

Including the following 2 domains:

1. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
   EC=2.7.7.60
   Alternative name(s):
   4-diphosphocytidyl-2C-methyl-D-erythritol synthase
   MEP cytidylyltransferase
   Short name=MCT
2. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
   Short name=MECDP-synthase
   Short name=MECPS
   EC=4.6.1.12

Gene names

Name:	ispDF
Ordered Locus Names:	HPSH_02215

Organism

Helicobacter pylori (strain Shi470) [Complete proteome] [HAMAP]

Taxonomic identifier

512562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (IspF) By similarity. HAMAP MF_01520
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520
Cofactor	Divalent metal cations By similarity. HAMAP MF_01520
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Sequence similarities	In the N-terminal section; belongs to the IspD family. In the C-terminal section; belongs to the IspF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: EC 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Bifunctional enzyme IspD/IspF HAMAP MF_01520		PRO_1000146274
Regions
	Region	1 – 247	247	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
	Region	248 – 406	159	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520
Sites
	Metal binding	254	1	Divalent metal cation By similarity
	Metal binding	256	1	Divalent metal cation By similarity
	Metal binding	288	1	Divalent metal cation By similarity
	Site	48	1	Transition state stabilizer By similarity
	Site	55	1	Transition state stabilizer By similarity
	Site	175	1	Positions MEP for the nucleophilic attack By similarity
	Site	227	1	Positions MEP for the nucleophilic attack By similarity
	Site	280	1	Transition state stabilizer By similarity
	Site	379	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2USR1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 8EF36BDEF1FDEC6C	FASTA	406	45,541
	10 20 30 40 50 60 MSLIRVNGEA FKLSLESLEE DPFENKETLE TLVKQTSVVL LAAGESRRFS QTIKKQWLRS 70 80 90 100 110 120 NHTPLWLSVY ESFKEALNFK EILLVVSELD YIYIKRHHPE IKLVRGGASR QESVRNALKI 130 140 150 160 170 180 IDSAYTLTSD VARGLANIEA LKSLFLTLQQ TSHYCIAPYL PCYDTAIYYN EVLDREAIKL 190 200 210 220 230 240 IQTPQLSHTK TLQSALNQGD FKDESSAILQ AFPDLVSYIE GSKDLHKLTT SDDLKHFALF 250 260 270 280 290 300 FNPAKDTFIG MGFDTHAFIK DKPMVLGGVV LDCEFGLKAH SDGDALLHAV IDAILGAIKG 310 320 330 340 350 360 GDIGEWFPDN DPQYKNASSK ELLKIVLDFS QSIGFELLEM GATIFSEIPK ITPYKPTILE 370 380 390 400 NLSQLLGLEK SQISLKATTM EKMGFIGKQE GLLVQAHVSM RYKQKL

References

[1]	"Genome sequence of Helicobacter pylori from the remote Amazon: traces of Asian ancestry of the first Americans." Kersulyte D., Kalia A., Gilman R.H., Berg D.E. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Shi470.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001072 Genomic DNA. Translation: ACD47893.1.
RefSeq	YP_001909923.1. NC_010698.2.
3D structure databases
ProteinModelPortal	B2USR1.
ModBase	Search...
Protein-protein interaction databases
STRING	B2USR1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6296321.
GenomeReviews	Gene locus HPSH_02215 in contig CP001072_GR.
KEGG	hps:HPSH_02215.
PATRIC	20612233. VBIHelPyl23559_0430.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	GGDIGEW.
ProtClustDB	PRK09382.
Family and domain databases
HAMAP	MF_01520. IspDF. [Tree]
InterPro	IPR023423. IpsF_dom. IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase. IPR020555. MECDP_synthase_CS. [Graphical view]
Gene3D	G3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
KO	K12506.
Pfam	PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view]
SUPFAM	SSF69765. YgbB_synth. 1 hit.
TIGRFAMs	TIGR00151. IspF. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. PS01350. ISPF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPDF_HELPS

Accession

Primary (citable) accession number: B2USR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program