B2US32 (FABH_HELPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3
EC=2.3.1.180

Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	HPSH_01035

Organism

Helicobacter pylori (strain Shi470) [Complete proteome] [HAMAP]

Taxonomic identifier

512562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm By similarity HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the FabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: InterPro beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000187870
Regions
	Region	256 – 260	5	ACP-binding By similarity
Sites
	Active site	115	1	By similarity
	Active site	255	1	By similarity
	Active site	285	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2US32 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: C0DAE6ABBCD1A403	FASTA	331	36,494
	10 20 30 40 50 60 MEFYASLKSI AMHVPSERVK NAEFQQFLDT SDEWIEKRTG IKERRFANDE EKSSDLGVIA 70 80 90 100 110 120 AKQAIERAHL TPKDIDLVVV ATLSPDFLAM PSTACVLSAK LGIENKPAFD ISAACTGFIY 130 140 150 160 170 180 LLSVAKAYVE SGMCENVLIV GAEKTSSVLD FKDRGTCILF GDGAGACVIG RTKRLKESVL 190 200 210 220 230 240 DVQISANGNF SNYLYTPRTL KPTPFNAKEE ASEPFLRMKG NEVFKLAVKT LLKDVEMILE 250 260 270 280 290 300 KNALKPEDVR LFIPHQANFR IIQAVREHLD FKDEQVVLTV HKYGNTSAAS IPMAMCEAYE 310 320 330 EGRLKKGDLM LLDAFGGGLT WGSALVYFGG S

References

[1]	"Genome sequence of Helicobacter pylori from the remote Amazon: traces of Asian ancestry of the first Americans." Kersulyte D., Kalia A., Gilman R.H., Berg D.E. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Shi470.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001072 Genomic DNA. Translation: ACD47664.1.
RefSeq	YP_001909694.1. NC_010698.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2US32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6296199.
GenomeReviews	Gene locus HPSH_01035 in contig CP001072_GR.
KEGG	hps:HPSH_01035.
PATRIC	20611765. VBIHelPyl23559_0200.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649927.
OMA	FNAKEEA.
ProtClustDB	PRK09352.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KO	K00648.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. FabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_HELPS

Accession

Primary (citable) accession number: B2US32

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program