B2URJ0 (CARA_AKKM8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbamoyl-phosphate synthase small chain
EC=6.3.5.5

Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	Amuc_1250

Organism

Akkermansia muciniphila (strain ATCC BAA-835) [Complete proteome] [HAMAP]

Taxonomic identifier

349741 [NCBI]

Taxonomic lineage

Bacteria › Verrucomicrobia › Verrucomicrobiae › Verrucomicrobiales › Verrucomicrobiaceae › Akkermansia

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the CarA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_1000138847
Regions
	Domain	183 – 369	187	Glutamine amidotransferase type-1
	Region	1 – 179	179	CPSase HAMAP MF_01209
Sites
	Active site	258	1	Nucleophile By similarity
	Active site	342	1	By similarity
	Active site	344	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2URJ0 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: F59D2E24481FE3EF	FASTA	372	40,193
	10 20 30 40 50 60 MRAILALEDG SVFIGSHFGA TGTEVGEACF NTSMTGYQEV LTDPSYSGQI VTMTYPLIGN 70 80 90 100 110 120 YGVNPEDGES GKAQVSGFVV AELAKVHSNW RATESLGPWL EKQGVIGIEG VDTRKIAKHL 130 140 150 160 170 180 RSAGAMRACL TTELTPEEAV EAARNAPSME GCNIVDKIGS HPMTPEEVDA LVTGKTLPPA 190 200 210 220 230 240 EYDIVAFDFG IKYNILRQLR ENGFRVTVVP AHTTAEEVLA MNPDGVFLSN GPGDPATLTD 250 260 270 280 290 300 IHREVAALIG KVPMFGICLG HQIISHALGA KTFKLKFGHR GANHPVKDLK TGKISITSQN 310 320 330 340 350 360 HGFAVDPDTV PDDVEITLIN LNDNTVEGIA HRTLPVFSVQ YHPEAAPGPR DPQYLFRDFR 370 KMIAASKRQH AR

References

[1]

"Complete sequence of Akkermansia muciniphila ATCC BAA-835." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N. , Smidt H., Derrien M., Plugge C.M., Zoetendal E.G., de Vos W.M., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-835.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001071 Genomic DNA. Translation: ACD05075.1.
RefSeq	YP_001877856.1. NC_010655.1.
3D structure databases
ProteinModelPortal	B2URJ0.
ModBase	Search...
Protein-protein interaction databases
STRING	B2URJ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6274736.
GenomeReviews	Gene locus Amuc_1250 in contig CP001071_GR.
KEGG	amu:Amuc_1250.
PATRIC	20835354. VBIAkkMuc16855_1415.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286341.
OMA	FTYPELG.
Family and domain databases
HAMAP	MF_01209. CPSase_S_chain. [Tree]
InterPro	IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR017926. GATASE_1. [Graphical view]
Gene3D	G3DSA:3.50.30.20. G3DSA:3.50.30.20. 1 hit.
KO	K01956.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
SMART	SM01097. CPSase_sm_chain. 1 hit. [Graphical view]
SUPFAM	SSF52021. CP_synthsmall. 1 hit.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_AKKM8

Accession

Primary (citable) accession number: B2URJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program